LOCUS       40924_34216        6857 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_012_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6857)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6857)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(475..1275)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1276..1496)
                     /label=URA3 promoter
     rep_origin      complement(2095..2975)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3324..4115)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4116..4220)
                     /label=AmpR promoter
     regulatory      4376..5135
                     /label=GPM1 promoter
                     /note="GPM1 promoter"
                     /regulatory_class="promoter"
     CDS             5144..5857
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5869..6268
                     /label=GPM1 terminator
                     /note="GPM1 terminator"
                     /regulatory_class="terminator"
     promoter        6275..6495
                     /label=URA3 promoter
     rep_origin      6616..6857
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
       61 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      361 cggccttttt acggttcctg gccttttgct ggccttttgc tcatgatata attaaattga
      421 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
      481 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
      541 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
      601 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
      661 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
      721 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
      781 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
      841 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
      901 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
      961 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
     1021 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
     1081 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
     1141 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
     1201 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
     1261 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
     1321 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
     1381 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
     1441 tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc
     1501 tagcttatcg atgataagct gtcaaagatg agaattaatt ccacggacta tagactatac
     1561 tagatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
     1621 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
     1681 tatcttcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca
     1741 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt
     1801 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat
     1861 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
     1921 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
     1981 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
     2041 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
     2101 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     2161 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     2221 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgacgaga gcgctaattt
     2281 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     2341 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     2401 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     2461 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     2521 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     2581 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     2641 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     2701 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     2761 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     2821 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     2881 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     2941 ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa gctccacccc ggttgataat
     3001 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aattctttcc
     3061 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3121 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagggtct
     3181 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3301 gagtaaactt ggtctgacag tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     3361 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     3421 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     3481 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     3541 caggcatcgt catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     3601 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     3661 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     3721 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     3781 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     3841 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     3901 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     3961 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     4021 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     4081 ggagaacctg cgtgcaatcc atcttgttca atcatactct tcctttttca atattattga
     4141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcgga cggatcgctt
     4261 gcctgtaact tacacgcgcc tcgtggcgcg cccctgcagg atttaaatgg ccggccgttt
     4321 aaaccttgta cacctaccgg tcaccagcca acaatgtgcg gatggcgtta caacttagtc
     4381 gtgcaatgta tgactttaag atttgtgagc aggaagaaaa gggagaatct tctaacgata
     4441 aacccttgaa aaactgggta gactacgcta tgttgagttg ctacgcaggc tgcacaatta
     4501 cacgagaatg ctcccgccta ggatttaagg ctaagggacg tgcaatgcag acgacagatc
     4561 taaatgaccg tgtcggtgaa gtgttcgcca aacttttcgg ttaacacatg cagtgatgca
     4621 cgcgcgatgg tgctaagtta catatatata tatatatata tatatatata tatatagcca
     4681 tagtgatgtc taagtaacct ttatggtata tttcttaatg tggaaagata ctagcgcgcg
     4741 cacccacaca caagcttcgt cttttcttga agaaaagagg aagctcgcta aatgggattc
     4801 cactttccgt tccctgccag ctgatggaaa aaggttagtg gaacgatgaa gaataaaaag
     4861 agagatccac tgaggtgaaa tttcagctga cagcgagttt catgatcgtg atgaacaatg
     4921 gtaacgagtt gtggctgttg ccagggaggg tggttctcaa cttttaatgt atggccaaat
     4981 cgctacttgg gtttgttata taacaaagaa gaaataatga actgattctc ttcctccttc
     5041 ttgtcctttc ttaattctgt tgtaattacc ttcctttgta attttttttg taattattct
     5101 tcttaataat ccaaacaaac acacatatta caatagcggc cgcatgtcta aaggtgaaga
     5161 attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa
     5221 attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga ccttaaaatt
     5281 tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta ctttcggtta
     5341 tggtgttcaa tgttttgcta gatacccaga tcatatgaaa caacatgact ttttcaagtc
     5401 tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta
     5461 caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa
     5521 aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa
     5581 ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa
     5641 aattagacac aacattgaag atggttctgt tcaattagct gaccattatc aacaaaatac
     5701 tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca ctcaatctgc
     5761 cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat ttgttactgc
     5821 tgctggtatt acccatggta tggatgaatt gtacaaataa gcggccgcgt ctgaagaatg
     5881 aatgatttga tgatttcttt ttccctccat ttttcttact gaatatatca atgatataga
     5941 cttgtatagt ttattatttc aaattaagta gctatatata gtcaagataa cgtttgtttg
     6001 acacgattac attattcgtc gacatctttt ttcagcctgt cgtggtagca atttgaggag
     6061 tattattaat tgaataggtt cattttgcgc tcgcataaac agttttcgtc agggacagta
     6121 tgttggaatg agtggtaatt aatggtgaca tgacatgtta tagcaataac cttgatgttt
     6181 acatcgtagt ttaatgtaca ccccgcgaat tcgttcaagt aggagtgcac caattgcaaa
     6241 gggaaaagct gaatgggcag ttcgaatata gcttttcaat tcaattcatc attttttttt
     6301 tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag
     6361 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     6421 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
     6481 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     6541 cctgtttgag atttaaatgg cgcgccgttt aaaccctgca ggggccggcc ccgtagaaaa
     6601 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6661 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6721 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6781 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6841 gttaccagtg gctgctg
//
