LOCUS       40924_34201        6897 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_009_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6897)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(478..1278)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1279..1499)
                     /label=URA3 promoter
     rep_origin      complement(2098..2978)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3327..4118)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4119..4223)
                     /label=AmpR promoter
     regulatory      4379..5178
                     /label=PDC1 promoter
                     /note="PDC1 promoter"
                     /regulatory_class="promoter"
     CDS             5187..5900
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5912..6311
                     /label=PDC1 terminator
                     /note="PDC1 terminator"
                     /regulatory_class="terminator"
     promoter        6318..6538
                     /label=URA3 promoter
     rep_origin      6659..6897
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
       61 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcatgat ataattaaat
      421 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
      481 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
      541 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
      601 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
      661 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
      721 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
      781 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
      841 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
      901 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
      961 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     1021 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     1081 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     1141 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     1201 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     1261 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     1321 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     1381 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     1441 atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
     1501 agctagctta tcgatgataa gctgtcaaag atgagaatta attccacgga ctatagacta
     1561 tactagatac tccgtctact gtacgataca cttccgctca ggtccttgtc ctttaacgag
     1621 gccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg tgatctaaga
     1681 ttctatcttc gcgatgtagt aaaactagct agaccgagaa agagactaga aatgcaaaag
     1741 gcacttctac aatggctgcc atcattatta tccgatgtga cgctgcagct tctcaatgat
     1801 attcgaatac gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac
     1861 gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa
     1921 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     1981 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     2041 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     2101 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     2161 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     2221 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgacg agagcgctaa
     2281 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     2341 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     2401 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     2461 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     2521 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     2581 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     2641 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     2701 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     2761 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     2821 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     2881 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     2941 tacttttgag caatgtttgt ggaagcggta ttcgcaatgg gaagctccac cccggttgat
     3001 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaattctt
     3061 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggg
     3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3301 tatgagtaaa cttggtctga cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg
     3361 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     3421 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     3481 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     3541 aagcaggcat cgtcatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     3601 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3661 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3721 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3781 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     3841 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     3901 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     3961 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4021 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4081 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat
     4141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ggacggatcg
     4261 cttgcctgta acttacacgc gcctcgtggc gcgcccctgc aggatttaaa tggccggccg
     4321 tttaaacctt gtacacctac cggtcaccag ccaacaatgt gcggatggcg ttacaactca
     4381 tgcgactggg tgagcatatg ttccgctgat gtgatgtgca agataaacaa gcaaggcaga
     4441 aactaacttc ttcttcatgt aataaacaca ccccgcgttt atttacctat ctctaaactt
     4501 caacacctta tatcataact aatatttctt gagataagca cactgcaccc ataccttcct
     4561 taaaaacgta gcttccagtt tttggtggtt ccggcttcct tcccgattcc gcccgctaaa
     4621 cgcatatttt tgttgcctgg tggcatttgc aaaatgcata acctatgcat ttaaaagatt
     4681 atgtatgctc ttctgacttt tcgtgtgatg aggctcgtgg aaaaaatgaa taatttatga
     4741 atttgagaac aattttgtgt tgttacggta ttttactatg gaataatcaa tcaattgagg
     4801 attttatgca aatatcgttt gaatattttt ccgacccttt gagtactttt cttcataatt
     4861 gcataatatt gtccgctgcc cctttttctg ttagacggtg tcttgatcta cttgctatcg
     4921 ttcaacacca ccttattttc taactatttt ttttttagct catttgaatc agcttatggt
     4981 gatggcacat ttttgcataa acctagctgt cctcgttgaa cataggaaaa aaaaatatat
     5041 aaacaaggct ctttcactct ccttgcaatc agatttgggt ttgttccctt tattttcata
     5101 tttcttgtca tattcctttc tcaattatta ttttctactc ataacctcac gcaaaataac
     5161 acagtcaaat caatcaaagc ggccgcatgt ctaaaggtga agaattattc actggtgttg
     5221 tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct gtctccggtg
     5281 aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt actactggta
     5341 aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt caatgttttg
     5401 ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg ccagaaggtt
     5461 atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc agagctgaag
     5521 tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt gattttaaag
     5581 aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac aatgtttaca
     5641 tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga cacaacattg
     5701 aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt ggtgatggtc
     5761 cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc aaagatccaa
     5821 acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt attacccatg
     5881 gtatggatga attgtacaaa taagcggccg cgcgatttaa tctctaatta ttagttaaag
     5941 ttttataagc atttttatgt aacgaaaaat aaattggttc atattattac tgcactgtca
     6001 cttaccatgg aaagaccaga caagaagttg ccgacagtct gttgaattgg cctggttagg
     6061 cttaagtctg ggtccgcttc tttacaaatt tggagaattt ctcttaaacg atatgtatat
     6121 tcttttcgtt ggaaaagatg tcttccaaaa aaaaaaccga tgaattagtg gaaccaagga
     6181 aaaaaaaaga ggtatccttg attaaggaac actgtttaaa cagtgtggtt tccaaaaccc
     6241 tgaaactgca ttagtgtaat agaagactag acacctcgat acaaataatg gttactcaat
     6301 tcaaaactgc ctagcttttc aattcaattc atcatttttt ttttattctt ttttttgatt
     6361 tcggtttctt tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
     6421 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
     6481 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
     6541 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttt gagatttaaa
     6601 tggcgcgccg tttaaaccct gcaggggccg gccccgtaga aaagatcaaa ggatcttctt
     6661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctg
//
