LOCUS       40924_34196        6690 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_008_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6690)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(474..1274)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1275..1495)
                     /label=URA3 promoter
     rep_origin      complement(2094..2974)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3323..4114)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4115..4219)
                     /label=AmpR promoter
     regulatory      4375..4974
                     /label=ENO2 promoter
                     /note="ENO2 promoter"
                     /regulatory_class="promoter"
     CDS             4983..5696
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5708..6100
                     /label=ENO2 terminator
                     /note="ENO2 terminator"
                     /regulatory_class="terminator"
     promoter        6107..6327
                     /label=URA3 promoter
     rep_origin      6448..6690
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
       61 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
      301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
      361 ggccttttta cggttcctgg ccttttgctg gccttttgct catgatataa ttaaattgaa
      421 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
      481 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
      541 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
      601 gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa
      661 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
      721 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
      781 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
      841 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
      901 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
      961 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa
     1021 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
     1081 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
     1141 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
     1201 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     1261 gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt
     1321 aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta
     1381 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     1441 caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagct
     1501 agcttatcga tgataagctg tcaaagatga gaattaattc cacggactat agactatact
     1561 agatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt aacgaggcct
     1621 taccactctt ttgttactct attgatccag ctcagcaaag gcagtgtgat ctaagattct
     1681 atcttcgcga tgtagtaaaa ctagctagac cgagaaagag actagaaatg caaaaggcac
     1741 ttctacaatg gctgccatca ttattatccg atgtgacgct gcagcttctc aatgatattc
     1801 gaatacgctt tgaggagata cagcctaata tccgacaaac tgttttacag atttacgatc
     1861 gtacttgtta cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag
     1921 atagtatatt tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat
     1981 gtatttcggt tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat
     2041 ccccggttca ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttaacgaag
     2101 catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     2161 agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac
     2221 gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgacgagag cgctaatttt
     2281 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt
     2341 ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta
     2401 tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt
     2461 ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc
     2521 tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga
     2581 agctgcgggt gcattttttc aagataaagg catccccgat tatattctat accgatgtgg
     2641 attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa
     2701 ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt
     2761 cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt
     2821 aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg
     2881 aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca aagagatact
     2941 tttgagcaat gtttgtggaa gcggtattcg caatgggaag ctccaccccg gttgataatc
     3001 agaaaagccc caaaaacagg aagattgtat aagcaaatat ttaaattgta attctttcct
     3061 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3121 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagggtctg
     3181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3301 agtaaacttg gtctgacagt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     3361 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     3421 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     3481 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     3541 aggcatcgtc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg
     3601 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     3661 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     3721 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     3781 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     3841 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     3901 tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt
     3961 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     4021 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     4081 gagaacctgc gtgcaatcca tcttgttcaa tcatactctt cctttttcaa tattattgaa
     4141 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4201 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcggac ggatcgcttg
     4261 cctgtaactt acacgcgcct cgtggcgcgc ccctgcagga tttaaatggc cggccgttta
     4321 aaccttgtac acctaccggt caccagccaa caatgtgcgg atggcgttac aactgtgtcg
     4381 acgctgcggg tatagaaagg gttctttact ctatagtacc tcctcgctca gcatctgctt
     4441 cttcccaaag atgaacgcgg cgttatgtca ctaacgacgt gcaccaactt gcggaaagtg
     4501 gaatcccgtt ccaaaactgg catccactaa ttgatacatc tacacaccgc acgccttttt
     4561 tctgaagccc actttcgtgg actttgccat atgcaaaatt catgaagtgt gataccaagt
     4621 cagcatacac ctcactaggg tagtttcttt ggttgtattg atcatttggt tcatcgtggt
     4681 tcattaattt tttttctcca ttgctttctg gctttgatct tactatcatt tggatttttg
     4741 tcgaaggttg tagaattgta tgtgacaagt ggcaccaagc atatataaaa aaaaaaagca
     4801 ttatcttcct accagagttg attgttaaaa acgtatttat agcaaacgca attgtaatta
     4861 attcttattt tgtatctttt cttcccttgt ctcaatcttt tatttttatt ttatttttct
     4921 tttcttagtt tctttcataa caccaagcaa ctaatactat aacatacaat aatagcggcc
     4981 gcatgtctaa aggtgaagaa ttattcactg gtgttgtccc aattttggtt gaattagatg
     5041 gtgatgttaa tggtcacaaa ttttctgtct ccggtgaagg tgaaggtgat gctacttacg
     5101 gtaaattgac cttaaaattt atttgtacta ctggtaaatt gccagttcca tggccaacct
     5161 tagtcactac tttcggttat ggtgttcaat gttttgctag atacccagat catatgaaac
     5221 aacatgactt tttcaagtct gccatgccag aaggttatgt tcaagaaaga actatttttt
     5281 tcaaagatga cggtaactac aagaccagag ctgaagtcaa gtttgaaggt gataccttag
     5341 ttaatagaat cgaattaaaa ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca
     5401 aattggaata caactataac tctcacaatg tttacatcat ggctgacaaa caaaagaatg
     5461 gtatcaaagt taacttcaaa attagacaca acattgaaga tggttctgtt caattagctg
     5521 accattatca acaaaatact ccaattggtg atggtccagt cttgttacca gacaaccatt
     5581 acttatccac tcaatctgcc ttatccaaag atccaaacga aaagagagac cacatggtct
     5641 tgttagaatt tgttactgct gctggtatta cccatggtat ggatgaattg tacaaataag
     5701 cggccgcagt gcttttaact aagaattatt agtcttttct gcttattttt tcatcatagt
     5761 ttagaacact ttatattaac gaatagttta tgaatctatt taggtttaaa aattgataca
     5821 gttttataag ttactttttc aaagactcgt gctgtctatt gcataatgca ctggaagggg
     5881 aaaaaaaagg tgcacacgcg tggctttttc ttgaatttgc agtttgaaaa ataactacat
     5941 ggatgataag aaaacatgga gtacagtcac tttgagaacc ttcaatcagc tggtaacgtc
     6001 ttcgttaatt ggatactcaa aaaagatgga tagcatgaat cacaagatgg aaggaaatgc
     6061 gggccacgac cacagtgata tgcatatggg agatggagat tagcttttca attcaattca
     6121 tcattttttt tttattcttt tttttgattt cggtttcttt gaaatttttt tgattcggta
     6181 atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt atatatacgc
     6241 atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact gcacagaaca
     6301 aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga acgtgctgct
     6361 actcatccta gtcctgtttg agatttaaat ggcgcgccgt ttaaaccctg caggggccgg
     6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6661 tctgctaatc ctgttaccag tggctgctgc
//
