LOCUS       40924_34186        6757 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_006_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6757)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(477..1277)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1278..1498)
                     /label=URA3 promoter
     rep_origin      complement(2097..2977)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3326..4117)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4118..4222)
                     /label=AmpR promoter
     regulatory      4378..4937
                     /label=TEF2 promoter
                     /note="TEF2 promoter"
                     /regulatory_class="promoter"
     CDS             4946..5659
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5671..6170
                     /label=TEF2 terminator
                     /note="TEF2 terminator"
                     /regulatory_class="terminator"
     promoter        6177..6397
                     /label=URA3 promoter
     rep_origin      6518..6757
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
       61 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcatgata taattaaatt
      421 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
      481 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
      541 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
      601 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
      661 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
      721 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
      781 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
      841 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
      901 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
      961 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
     1021 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
     1081 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
     1141 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
     1201 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
     1261 tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg
     1321 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat
     1381 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa
     1441 tttcaaagaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa
     1501 gctagcttat cgatgataag ctgtcaaaga tgagaattaa ttccacggac tatagactat
     1561 actagatact ccgtctactg tacgatacac ttccgctcag gtccttgtcc tttaacgagg
     1621 ccttaccact cttttgttac tctattgatc cagctcagca aaggcagtgt gatctaagat
     1681 tctatcttcg cgatgtagta aaactagcta gaccgagaaa gagactagaa atgcaaaagg
     1741 cacttctaca atggctgcca tcattattat ccgatgtgac gctgcagctt ctcaatgata
     1801 ttcgaatacg ctttgaggag atacagccta atatccgaca aactgtttta cagatttacg
     1861 atcgtacttg ttacccatca ttgaattttg aacatccgaa cctgggagtt ttccctgaaa
     1921 cagatagtat atttgaacct gtataataat atatagtcta gcgctttacg gaagacaatg
     1981 tatgtatttc ggttcctgga gaaactattg catctattgc ataggtaatc ttgcacgtcg
     2041 catccccggt tcattttctg cgtttccatc ttgcacttca atagcatatc tttgttaacg
     2101 aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa
     2161 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc
     2221 aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgacga gagcgctaat
     2281 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     2341 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     2401 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     2461 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     2521 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     2581 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     2641 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     2701 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     2761 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     2821 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     2881 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     2941 acttttgagc aatgtttgtg gaagcggtat tcgcaatggg aagctccacc ccggttgata
     3001 atcagaaaag ccccaaaaac aggaagattg tataagcaaa tatttaaatt gtaattcttt
     3061 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3121 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagggt
     3181 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3241 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3301 atgagtaaac ttggtctgac agtcagaaga actcgtcaag aaggcgatag aaggcgatgc
     3361 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     3421 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     3481 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     3541 agcaggcatc gtcatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     3601 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     3661 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     3721 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     3781 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     3841 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     3901 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     3961 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     4021 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     4081 ccggagaacc tgcgtgcaat ccatcttgtt caatcatact cttccttttt caatattatt
     4141 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4201 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgcg gacggatcgc
     4261 ttgcctgtaa cttacacgcg cctcgtggcg cgcccctgca ggatttaaat ggccggccgt
     4321 ttaaaccttg tacacctacc ggtcaccagc caacaatgtg cggatggcgt tacaactggg
     4381 gccgtatact tacatatagt agatgtcaag cgtaggcgct tcccctgccg gctgtgaggg
     4441 cgccataacc aaggtatcta tagaccgcca atcagcaaac tacctccgta cattcatgtt
     4501 gcacccacac atttatacac ccagaccgcg acaaattacc cataaggttg tttgtgacgg
     4561 cgtcgtacaa gagaacgtgg gaacttttta ggctcaccaa aaaagaaaga aaaaatacga
     4621 gttgctgaca gaagcctcaa gaaaaaaaaa attcttcttc gactatgctg gaggcagaga
     4681 tgatcgagcc ggtagttaac tatatatagc taaattggtt ccatcacctt cttttctggt
     4741 gtcgctcctt ctagtgctat ttctggcttt tcctattttt ttttttccat ttttctttct
     4801 ctctttctaa tatataaatt ctcttgcatt ttctattttt ctctctatct attctacttg
     4861 tttattccct tcaaggtttt tttttaagga gtacttgttt ttagaatata cggtcaacga
     4921 actataatta actaaacgcg gccgcatgtc taaaggtgaa gaattattca ctggtgttgt
     4981 cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg tctccggtga
     5041 aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta ctactggtaa
     5101 attgccagtt ccatggccaa ccttagtcac tactttcggt tatggtgttc aatgttttgc
     5161 tagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc cagaaggtta
     5221 tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca gagctgaagt
     5281 caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg attttaaaga
     5341 agatggtaac attttaggtc acaaattgga atacaactat aactctcaca atgtttacat
     5401 catggctgac aaacaaaaga atggtatcaa agttaacttc aaaattagac acaacattga
     5461 agatggttct gttcaattag ctgaccatta tcaacaaaat actccaattg gtgatggtcc
     5521 agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca aagatccaaa
     5581 cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta ttacccatgg
     5641 tatggatgaa ttgtacaaat aagcggccgc gagtaataat tattgcttcc atataatatt
     5701 tttatatacc tcttattttt atgtattagt taattaagta tttttatcta tctgcttatc
     5761 attttctttt catatagggg gggttggtgt tttcttgccc atcagattga tgtcctccaa
     5821 ctcggcacta ttttacaaag ggtttttttg taagagaagg agaagacaga tactaaacca
     5881 tacgttactc gaaacaaaaa aaaaaaaaat ggaaaaagct gctatcaaca aaagacggcc
     5941 tcatcaaacc taaagaaacc atgtcagcgt atgtatatac cttgtaattt acgtttcctt
     6001 aaatcttctt tctactaacg ttttcattat tctatactct atgaccaata aaaacagact
     6061 gtactttcaa aatttaccca gtaggccagc aaataaagaa aattatacca gattacttct
     6121 gaaacacatt aatcccaaca acaagtatgc cattaatccg tcgctacccc tagcttttca
     6181 attcaattca tcattttttt tttattcttt tttttgattt cggtttcttt gaaatttttt
     6241 tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt
     6301 atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact
     6361 gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta catataagga
     6421 acgtgctgct actcatccta gtcctgtttg agatttaaat ggcgcgccgt ttaaaccctg
     6481 caggggccgg ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6721 catacctcgc tctgctaatc ctgttaccag tggctgc
//
