LOCUS       40924_34181        6635 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_005_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6635)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6635)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(483..1283)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1284..1504)
                     /label=URA3 promoter
     rep_origin      complement(2103..2983)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3332..4123)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4124..4228)
                     /label=AmpR promoter
     regulatory      4384..5133
                     /label=PGK1 promoter
                     /note="PGK1 promoter"
                     /regulatory_class="promoter"
     CDS             5142..5855
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5867..6054
                     /label=PGK1 terminator
                     /note="PGK1 terminator"
                     /regulatory_class="terminator"
     promoter        6061..6281
                     /label=URA3 promoter
     rep_origin      6402..6635
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
       61 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc atgatataat
      421 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt
      481 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca
      541 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca
      601 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg
      661 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc
      721 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca
      781 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc
      841 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata
      901 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca
      961 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag
     1021 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa
     1081 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
     1141 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
     1201 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
     1261 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
     1321 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     1381 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     1441 aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat
     1501 tgaaaagcta gcttatcgat gataagctgt caaagatgag aattaattcc acggactata
     1561 gactatacta gatactccgt ctactgtacg atacacttcc gctcaggtcc ttgtccttta
     1621 acgaggcctt accactcttt tgttactcta ttgatccagc tcagcaaagg cagtgtgatc
     1681 taagattcta tcttcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc
     1741 aaaaggcact tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca
     1801 atgatattcg aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga
     1861 tttacgatcg tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc
     1921 ctgaaacaga tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag
     1981 acaatgtatg tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc
     2041 acgtcgcatc cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg
     2101 ttaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt
     2161 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat
     2221 tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgacgagagc
     2281 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     2341 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     2401 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     2461 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     2521 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     2581 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     2641 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     2701 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     2761 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     2821 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     2881 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     2941 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatgggaagc tccaccccgg
     3001 ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa
     3061 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3181 gagggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3241 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3301 gtatatatga gtaaacttgg tctgacagtc agaagaactc gtcaagaagg cgatagaagg
     3361 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     3421 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     3481 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     3541 tcggcaagca ggcatcgtca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct
     3601 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     3661 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     3721 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     3781 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     3841 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     3901 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     3961 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     4021 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     4081 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catactcttc ctttttcaat
     4141 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     4201 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcggacg
     4261 gatcgcttgc ctgtaactta cacgcgcctc gtggcgcgcc cctgcaggat ttaaatggcc
     4321 ggccgtttaa accttgtaca cctaccggtc accagccaac aatgtgcgga tggcgttaca
     4381 actacgcaca gatattataa catctgcata ataggcattt gcaagaatta ctcgtgagta
     4441 aggaaagagt gaggaactat cgcatacctg catttaaaga tgccgatttg ggcgcgaatc
     4501 ctttattttg gcttcaccct catactatta tcagggccag aaaaaggaag tgtttccctc
     4561 cttcttgaat tgatgttacc ctcataaagc acgtggcctc ttatcgagaa agaaattacc
     4621 gtcgctcgtg atttgtttgc aaaaagaaca aaactgaaaa aacccagaca cgctcgactt
     4681 cctgtcttcc tattgattgc agcttccaat ttcgtcacac aacaaggtcc tagcgacggc
     4741 tcacaggttt tgtaacaagc aatcgaaggt tctggaatgg cgggaaaggg tttagtacca
     4801 catgctatga tgcccactgt gatctccaga gcaaagttcg ttcgatcgta ctgttactct
     4861 ctctctttca aacagaattg tccgaatcgt gtgacaacaa cagcctgttc tcacacactc
     4921 ttttcttcta accaaggggg tggtttagtt tagtagaacc tcgtgaaact tacatttaca
     4981 tatatataaa cttgcataaa ttggtcaatg caagaaatac atatttggtc ttttctaatt
     5041 cgtagttttt caagttctta gatgctttct ttttctcttt tttacagatc atcaaggaag
     5101 taattatcta ctttttacaa caaatataaa acagcggccg catgtctaaa ggtgaagaat
     5161 tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat
     5221 tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta
     5281 tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact ttcggttatg
     5341 gtgttcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg
     5401 ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca
     5461 agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag
     5521 gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac aactataact
     5581 ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa
     5641 ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc
     5701 caattggtga tggtccagtc ttgttaccag acaaccatta cttatccact caatctgcct
     5761 tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg
     5821 ctggtattac ccatggtatg gatgaattgt acaaataagc ggccgcattg aattgaattg
     5881 aaatcgatag atcaattttt ttcttttctc tttccccatc ctttacgcta aaataatagt
     5941 ttattttatt ttttgaatat tttttattta tatacgtata tatagactat tatttatctt
     6001 ttaatgatta ttaagatttt tattaaaaaa aaattcgctc ctcttttaat gccttagctt
     6061 ttcaattcaa ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt
     6121 tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat
     6181 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc
     6241 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
     6301 aggaacgtgc tgctactcat cctagtcctg tttgagattt aaatggcgcg ccgtttaaac
     6361 cctgcagggg ccggccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6541 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6601 cctacatacc tcgctctgct aatcctgtta ccagt
//
