LOCUS       40924_34176        6496 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_004_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6496)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6496)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(471..1271)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1272..1492)
                     /label=URA3 promoter
     rep_origin      complement(2091..2971)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3320..4111)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4112..4216)
                     /label=AmpR promoter
     regulatory      4372..4769
                     /label=HXT7 promoter
                     /note="HXT7 promoter"
                     /regulatory_class="promoter"
     CDS             4778..5491
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5503..5903
                     /label=HXT7 terminator
                     /note="HXT7 terminator"
                     /regulatory_class="terminator"
     promoter        5910..6130
                     /label=URA3 promoter
     rep_origin      6251..6496
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
       61 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      361 ctttttacgg ttcctggcct tttgctggcc ttttgctcat gatataatta aattgaagct
      421 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
      481 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
      541 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
      601 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
      661 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
      721 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
      781 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
      841 ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
      901 acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
      961 tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     1021 tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     1081 atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     1141 aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     1201 atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     1261 gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag
     1321 aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt
     1381 ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa
     1441 agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctagc
     1501 ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga ctatactaga
     1561 tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggccttac
     1621 cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta agattctatc
     1681 ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc
     1741 tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat gatattcgaa
     1801 tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt tacgatcgta
     1861 cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata
     1921 gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta
     1981 tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc
     2041 cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat
     2101 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     2161 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     2221 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc taatttttca
     2281 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     2341 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     2401 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     2461 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     2521 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     2581 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     2641 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     2701 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     2761 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     2821 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     2881 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     2941 gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt gataatcaga
     3001 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaatt ctttcctgcg
     3061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gggtctgacg
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3301 aaacttggtc tgacagtcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3361 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     3421 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     3481 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     3541 catcgtcatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     3601 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     3661 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     3721 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     3781 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     3841 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     3901 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     3961 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4021 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4081 aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat tattgaagca
     4141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcggacgga tcgcttgcct
     4261 gtaacttaca cgcgcctcgt ggcgcgcccc tgcaggattt aaatggccgg ccgtttaaac
     4321 cttgtacacc taccggtcac cagccaacaa tgtgcggatg gcgttacaac tacttctcgt
     4381 aggaacaatt tcgggcccct gcgtgttctt ctgaggttca tcttttacat ttgcttctgc
     4441 tggataattt tcagaggcaa caaggaaaaa ttagatggca aaaagtcgtc tttcaaggaa
     4501 aaatccccac catctttcga gatcccctgt aacttattgg caactgaaag aatgaaaagg
     4561 aggaaaatac aaaatatact agaactgaaa aaaaaaaagt ataaatagag acgatatatg
     4621 ccaatacttc acaatgttcg aatctattct tcatttgcag ctattgtaaa ataataaaac
     4681 atcaagaaca aacaagctca acttgtcttt tctaagaaca aagaataaac acaaaaacaa
     4741 aaagtttttt taattttaat caaaaaatgg cggccgcatg tctaaaggtg aagaattatt
     4801 cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc
     4861 tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg
     4921 tactactggt aaattgccag ttccatggcc aaccttagtc actactttcg gttatggtgt
     4981 tcaatgtttt gctagatacc cagatcatat gaaacaacat gactttttca agtctgccat
     5041 gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac
     5101 cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat
     5161 tgattttaaa gaagatggta acattttagg tcacaaattg gaatacaact ataactctca
     5221 caatgtttac atcatggctg acaaacaaaa gaatggtatc aaagttaact tcaaaattag
     5281 acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa atactccaat
     5341 tggtgatggt ccagtcttgt taccagacaa ccattactta tccactcaat ctgccttatc
     5401 caaagatcca aacgaaaaga gagaccacat ggtcttgtta gaatttgtta ctgctgctgg
     5461 tattacccat ggtatggatg aattgtacaa ataagcggcc gctttgcgaa cacttttatt
     5521 aattcatgat cacgctctaa tttgtgcatt tgaaatgtac tctaattcta attttatatt
     5581 tttaatgata tcttgaaaag taaatacgtt tttaatatat acaaaataat acagtttaat
     5641 tttcaagttt ttgatcattt gttctcagaa agttgagtgg gacggagaca aagaaacttt
     5701 aaagagaaat gcaaagtggg aagaagtcag ttgtttaccg accgcactgt tattcacaaa
     5761 tattccaatt ttgcctgcag acccacgtct acaaattttg gttagtttgg taaatggtaa
     5821 ggatatagta gagccttttt gaaatgggaa atatcttctt tttctgtatc ccgcttcaaa
     5881 aagtgtctaa tgagtcagtt atttagcttt tcaattcaat tcatcatttt ttttttattc
     5941 ttttttttga tttcggtttc tttgaaattt ttttgattcg gtaatctccg aacagaagga
     6001 agaacgaagg aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga
     6061 aacatgaaat tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg
     6121 aagataaatc atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt
     6181 ttgagattta aatggcgcgc cgtttaaacc ctgcaggggc cggccccgta gaaaagatca
     6241 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6301 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6361 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6421 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6481 cagtggctgc tgccag
//
