LOCUS       40924_34171        6798 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_003_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6798)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6798)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(473..1273)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1274..1494)
                     /label=URA3 promoter
     rep_origin      complement(2093..2973)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3322..4113)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4114..4218)
                     /label=AmpR promoter
     regulatory      4374..5073
                     /label=TDH2 promoter
                     /note="TDH2 promoter"
                     /regulatory_class="promoter"
     CDS             5082..5795
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5807..6207
                     /label=TDH2 terminator
                     /note="TDH2 terminator"
                     /regulatory_class="terminator"
     promoter        6214..6434
                     /label=URA3 promoter
     rep_origin      6555..6798
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
       61 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      121 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      181 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      241 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      301 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      361 gcctttttac ggttcctggc cttttgctgg ccttttgctc atgatataat taaattgaag
      421 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
      481 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
      541 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
      601 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
      661 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
      721 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
      781 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
      841 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
      901 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
      961 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     1021 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     1081 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     1141 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     1201 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     1261 tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
     1321 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
     1381 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     1441 aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta
     1501 gcttatcgat gataagctgt caaagatgag aattaattcc acggactata gactatacta
     1561 gatactccgt ctactgtacg atacacttcc gctcaggtcc ttgtccttta acgaggcctt
     1621 accactcttt tgttactcta ttgatccagc tcagcaaagg cagtgtgatc taagattcta
     1681 tcttcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc aaaaggcact
     1741 tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca atgatattcg
     1801 aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga tttacgatcg
     1861 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
     1921 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
     1981 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
     2041 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
     2101 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     2161 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     2221 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgacgagagc gctaattttt
     2281 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     2341 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     2401 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     2461 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     2521 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     2581 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     2641 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     2701 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     2761 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     2821 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     2881 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     2941 ttgagcaatg tttgtggaag cggtattcgc aatgggaagc tccaccccgg ttgataatca
     3001 gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa ttctttcctg
     3061 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3121 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagggtctga
     3181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3301 gtaaacttgg tctgacagtc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     3361 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     3421 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     3481 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     3541 ggcatcgtca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
     3601 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     3661 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     3721 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     3781 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     3841 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     3901 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
     3961 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     4021 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     4081 agaacctgcg tgcaatccat cttgttcaat catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcggacg gatcgcttgc
     4261 ctgtaactta cacgcgcctc gtggcgcgcc cctgcaggat ttaaatggcc ggccgtttaa
     4321 accttgtaca cctaccggtc accagccaac aatgtgcgga tggcgttaca actattggtt
     4381 tttccagtga atgattattt gtcgttaccc tttcgtaaaa gttcaaacac gtttttaagt
     4441 attgtttagt tgctctttcg acatatatga ttatccctgc gcggctaaag ttaaggatgc
     4501 aaaaaacata agacaactga agttaattta cgtcaattaa gttttccagg gtaatgatgt
     4561 tttgggcttc cactaattca ataagtatgt catgaaatac gttgtgaaga gcatccagaa
     4621 ataatgaaaa gaaacaacga aactgggtcg gcctgttgtt tcttttcttt accacgtgat
     4681 ctgcggcatt tacaggaagt cgcgcgtttt gcgcagttgt tgcaacgcag ctacggctaa
     4741 caaagcctag tggaactcga ctgatgtgtt agggcctaaa actggtggtg acagctgaag
     4801 tgaactattc aatccaatca tgtcatggct gtcacaaaga ccttgcggac cgcacgtacg
     4861 aacacatacg tatgctaata tgtgttttga tagtacccag tgatcgcaga cctgcaattt
     4921 ttttgtaggt ttggaagaat atataaaggt tgcactcatt caagatagtt tttttcttgt
     4981 gtgtctattc attttattat tgtttgttta aatgttaaaa aaaccaagaa cttagtttca
     5041 aattaaattc atcacacaaa caaacaaaac aaagcggccg catgtctaaa ggtgaagaat
     5101 tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat
     5161 tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta
     5221 tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact ttcggttatg
     5281 gtgttcaatg ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg
     5341 ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca
     5401 agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag
     5461 gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac aactataact
     5521 ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa
     5581 ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc
     5641 caattggtga tggtccagtc ttgttaccag acaaccatta cttatccact caatctgcct
     5701 tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg
     5761 ctggtattac ccatggtatg gatgaattgt acaaataagc ggccgcattt aactccttaa
     5821 gttactttaa tgatttagtt tttattatta ataattcatg ctcatgacat ctcatataca
     5881 cgtttataaa acttaaatag attgaaaatg tattaaagat tcctcaggga ttcgattttt
     5941 ttggaagttt ttgttttttt ttccttgaga tgctgtagta tttgggaaca attatacaat
     6001 cgaaagatat atgcttacat tcgaccgttt tagccgtgat cattatccta tagtaacata
     6061 acctgaagca taactgacac tactatcatc aatacttgtc acatgagaac tctgtgaata
     6121 attaggccac tgaaatttga tgcctgaagg accggcatca cggattttcg ataaagcact
     6181 tagtatcaca ctaattggct tttcgcctag cttttcaatt caattcatca tttttttttt
     6241 attctttttt ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga
     6301 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
     6361 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga
     6421 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
     6481 ctgtttgaga tttaaatggc gcgccgttta aaccctgcag gggccggccc cgtagaaaag
     6541 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6601 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6661 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6721 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6781 ttaccagtgg ctgctgcc
//
