LOCUS       40924_34166        6548 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_002_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6548)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6548)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(478..1278)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1279..1499)
                     /label=URA3 promoter
     rep_origin      complement(2098..2978)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3327..4118)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4119..4223)
                     /label=AmpR promoter
     regulatory      4379..4779
                     /label=PGI1 promoter
                     /note="PGI1 promoter"
                     /regulatory_class="promoter"
     CDS             4788..5501
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5513..5962
                     /label=PGI1 terminator
                     /note="PGI1 terminator"
                     /regulatory_class="terminator"
     promoter        5969..6189
                     /label=URA3 promoter
     rep_origin      6310..6548
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
       61 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcatgat ataattaaat
      421 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
      481 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
      541 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
      601 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
      661 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
      721 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
      781 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
      841 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
      901 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
      961 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     1021 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     1081 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     1141 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     1201 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     1261 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     1321 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     1381 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     1441 atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
     1501 agctagctta tcgatgataa gctgtcaaag atgagaatta attccacgga ctatagacta
     1561 tactagatac tccgtctact gtacgataca cttccgctca ggtccttgtc ctttaacgag
     1621 gccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg tgatctaaga
     1681 ttctatcttc gcgatgtagt aaaactagct agaccgagaa agagactaga aatgcaaaag
     1741 gcacttctac aatggctgcc atcattatta tccgatgtga cgctgcagct tctcaatgat
     1801 attcgaatac gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac
     1861 gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa
     1921 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     1981 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     2041 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     2101 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     2161 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     2221 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgacg agagcgctaa
     2281 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     2341 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     2401 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     2461 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     2521 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     2581 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     2641 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     2701 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     2761 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     2821 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     2881 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     2941 tacttttgag caatgtttgt ggaagcggta ttcgcaatgg gaagctccac cccggttgat
     3001 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaattctt
     3061 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggg
     3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3301 tatgagtaaa cttggtctga cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg
     3361 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     3421 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     3481 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     3541 aagcaggcat cgtcatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     3601 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3661 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3721 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3781 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     3841 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     3901 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     3961 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4021 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4081 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat
     4141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ggacggatcg
     4261 cttgcctgta acttacacgc gcctcgtggc gcgcccctgc aggatttaaa tggccggccg
     4321 tttaaacctt gtacacctac cggtcaccag ccaacaatgt gcggatggcg ttacaactgt
     4381 aacaaaaatc acgatctggg tgggtgtggg tgtattggat tataggaagc cacgcgctca
     4441 acctggaatt acaggaagct ggtaattttt tgggtttgca atcatcacca tctgcacgtt
     4501 gttataatgt cccgtgtcta tatatatcca ttgacggtat tctatttttt tgctattgaa
     4561 atgagcgttt tttgttacta caattggttt tacagacgga attttcccta tttgtttcgt
     4621 cccatttttc cttttctcat tgttctcata tcttaaaaag gtcctttctt cataatcaat
     4681 gctttctttt acttaatatt ttacttgcat tcagtgaatt ttaatacata ttcctctagt
     4741 cttgcaaaat cgatttagaa tcaagatacc agcctaaaag cggccgcatg tctaaaggtg
     4801 aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc
     4861 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
     4921 aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttcg
     4981 gttatggtgt tcaatgtttt gctagatacc cagatcatat gaaacaacat gactttttca
     5041 agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta
     5101 actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat
     5161 taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg gaatacaact
     5221 ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc aaagttaact
     5281 tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa
     5341 atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta tccactcaat
     5401 ctgccttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta gaatttgtta
     5461 ctgctgctgg tattacccat ggtatggatg aattgtacaa ataagcggcc gcacaaatcg
     5521 ctcttaaata tatacctaaa gaacattaaa gctatattat aagcaaagat acgtaaattt
     5581 tgcttatatt attatacaca tatcatattt ctatattttt aagatttggt tatataatgt
     5641 acgtaatgca aaggaaataa attttataca ttattgaaca gcgtccaagt aactacatta
     5701 tgtgcactaa tagtttagcg tcgtgaagac tttattgtgt cgcgaaaagt aaaaatttta
     5761 aaaattagag caccttgaac ttgcgaaaaa ggttctcatc aactgtttaa aaggaggata
     5821 tcaggtccta tttctgacaa acaatataca aatttagttt caaagatgaa tcagtgcgcg
     5881 aaggacataa ctcatgaagc ctccagtata cccatcgatt tgcaagaaag atactcgcac
     5941 tggaagaaaa acactaaact actagctttt caattcaatt catcattttt tttttattct
     6001 tttttttgat ttcggtttct ttgaaatttt tttgattcgg taatctccga acagaaggaa
     6061 gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa
     6121 acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga
     6181 agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt
     6241 tgagatttaa atggcgcgcc gtttaaaccc tgcaggggcc ggccccgtag aaaagatcaa
     6301 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6421 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6541 agtggctg
//
