LOCUS       62056_18345        5659 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..127
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             160..1809
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1856..1977)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2171..2528
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2559..3350
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3378..3426
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3753..4341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4544..5401)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5568..5659
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacca cgtttgcctt gcctggactt gcctggcctt gccttggaca
       61 tgcccgggct gtcagatctg cagaagctta gacactagag ggtatataat ggaagctcga
      121 cttccagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      181 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      241 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      301 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      361 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      421 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      481 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      541 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      601 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      661 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      721 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      781 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      841 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      901 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      961 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
     1021 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1081 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1141 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1201 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1261 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1321 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1381 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1441 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1501 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1561 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1621 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1681 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1741 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1801 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1861 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1921 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1981 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2041 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
     2101 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     2161 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     2221 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2281 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2341 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2401 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2461 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2521 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc
     2581 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga
     2641 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt
     2701 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga
     2761 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg
     2821 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg
     2881 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc
     2941 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca
     3001 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg
     3061 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac
     3121 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg
     3181 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca
     3241 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag
     3301 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta
     3361 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     3421 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct
     3481 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     3541 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg
     3601 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3661 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4381 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4441 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4501 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg
     4561 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg
     4621 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc
     4681 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg
     4741 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg
     4801 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg
     4861 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt
     4921 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc
     4981 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac
     5041 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa
     5101 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac
     5161 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt
     5221 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc
     5281 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa
     5341 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca
     5401 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag
     5461 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta
     5521 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
     5581 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
     5641 ggtgccagaa catttctct
//
