LOCUS       62056_18315        3840 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1195
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             1199..1216
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             1223..1498
                     /codon_start=1
                     /label=SUMO3
                     /note="human small ubiquitin-related modifier 3"
                     /translation="LQEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     CDS             1567..1584
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1664..1910
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1925..2336
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2344..2391
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2410..2781
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2850..3097
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3172..3760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagaggtca
     1201 tcaccatcat catcacgggt ccctgcagga ggagaagccc aaggagggtg tgaagacaga
     1261 gaatgaccac atcaacctga aggtggccgg gcaggacggc tccgtggtgc agttcaagat
     1321 caagaggcac acgccgctga gcaagctgat gaaggcctac tgcgagaggc agggcttgtc
     1381 aatgaggcag atcagattca ggttcgacgg gcagccaatc aatgaaactg acactccagc
     1441 acagctggag atggaggacg aggacaccat cgacgtgttc cagcagcaga cgggaggtag
     1501 agacgatctg ccgtctctct agagcggccg cggtaccgaa ttccaacctg cgattgatct
     1561 gtcgaccatc atcatcatca tcattgagtt tgtagcctta gacatgactg ttcctcagtt
     1621 caagttgggc acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa
     1681 tgccatttgc ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat
     1741 agtataggat tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct
     1801 atctcgcagc tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt
     1861 ttttcttggt atttcccact cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg
     1921 tgcggatccc ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga
     1981 ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa
     2041 ttttccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga
     2101 aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac
     2161 gtttcttttt cttgaaattt ttttttttag tttttttctc tttcagtgac ctccattgat
     2221 atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt cttgttctat
     2281 tacaactttt tttacttctt gttcattaga aagaaagcat agcaatctaa tctaaggggc
     2341 ggtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     2401 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     2461 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     2521 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     2581 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     2641 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     2701 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     2761 ttcgtggccg aggagcagga ctgacacgtc cgacggcggc ccacgggtcc caggcctcgg
     2821 agatccgtcc cccttttcct ttgtcgatat catgtaatta gttatgtcac gcttacattc
     2881 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
     2941 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
     3001 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
     3061 tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag ctggagacca acatgtgagc
     3121 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3181 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3241 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3301 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3361 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3421 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3481 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3541 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3601 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3661 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3721 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3781 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatc
//
