LOCUS       62056_18305        4217 bp DNA     circular SYN 16-AUG-2021
DEFINITION  Cloning vector pOsym(core)_mKate2, complete sequence.
ACCESSION   MZ198786
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Groseclose TM, Hersey AN, Huang BD, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2021) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..74
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     regulatory      74..79
                     /label=trc promoter -10 hexamer
                     /note="trc promoter -10 hexamer"
                     /regulatory_class="promoter"
     regulatory      98..109
                     /label=strong bacterial RBS
                     /note="strong bacterial RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             116..811
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1453..2400)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2448..2670)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3162..3256)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3290..4081)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtccaac atatggcacc ggaggctttc gtcttcacct cgagaattgt ttgacattgt
       61 gagcgctcac aattataatc tgagcacaga attcattaaa gaggagaaag gtaccatggt
      121 atctgaactg atcaaggaaa acatgcatat gaagttgtat atggaaggta ccgtgaacaa
      181 ccatcatttc aagtgtacta gtgaaggtga aggaaaaccg tatgaaggaa ctcagacaat
      241 gcgtattaaa gctgtagaag gtggaccgct gccttttgct ttcgatatct tggcaacctc
      301 tttcatgtat ggtagtaaga ctttcatcaa ccatacacag ggaatcccgg atttctttaa
      361 acaatcgttt cctgaaggtt tcacatggga acgtgtaacc acttatgaag atggaggagt
      421 actgaccgcc actcaggata ccagcctgca agatggatgt ttgatctata acgtaaagat
      481 ccgtggtgtg aacttcccgt cgaatggacc tgtaatgcag aagaaaacct tgggttggga
      541 agccagcaca gaaaccctgt atccggctga tggaggactg gaaggacgtg cagatatggc
      601 cctgaaactg gtaggtggag gacatctgat ctgtaacttg aagacaacct atcgttctaa
      661 aaaaccggca aagaacctga agatgcctgg tgtatattat gtggatcgtc gtttggaacg
      721 tatcaaagaa gccgataaag aaacatatgt ggaacaacat gaagtagccg tggctcgtta
      781 ttgtgatctg ccttctaaat tgggacatcg ttaatctaga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct
      961 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc
     1021 tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     1081 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     1141 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     1201 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     1261 atctgttcat ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct
     1321 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg
     1381 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     1441 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     1501 ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa
     1561 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     1621 tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     1681 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     1741 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     1801 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     1861 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     1921 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     1981 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     2041 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     2101 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     2161 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     2221 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     2281 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     2341 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     2401 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     2461 ataattacta gtccttttcc cgggagatct gggtatctgt aaattctgct agacctttgc
     2521 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     2581 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     2641 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     2701 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     2761 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     2821 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     2881 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     2941 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     3001 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     3061 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     3121 tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac
     3181 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     3241 tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt
     3301 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     3361 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     3421 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     3481 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     3541 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     3601 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     3661 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     3721 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
     3781 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     3841 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     3901 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     3961 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     4021 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     4081 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc
     4141 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     4201 ccccagctgg caattcc
//
