LOCUS       40924_33957        1804 bp DNA     circular SYN 01-JAN-1980
DEFINITION  cDNA cloning vector derived from pOTB7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..1804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    85..109
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(243..266)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(282..300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      404..992
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1133..1789
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
       61 gacactatag aactcgaagg atccacaagt ttgtacaaaa aagcaggctt gtaaaacgac
      121 ggccagtaac tataacggtc ctaaggtagc gaggcctggg tggcctcgag ccttccgaat
      181 tctttcatta cctctttctc cgcaccccac ataaagtgcg gagaaagagg taatgaaatg
      241 gcacccagct ttcttgtaca agtggtagat ctgccggtct ccctatagtg agtcgtatta
      301 atttcgataa gccaggttaa cctgcattaa tgaatcggct gcagtacccg ggaatttaac
      361 ccgcctaatg agcgggcttt tttttgtgat ccaaaggatc ttcttgagat cctttttttc
      421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      541 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggat cacaacaaaa
     1021 agcccgctca ttaggcgggc taaattctca tgtttgacag cttatcatcg ataagctagc
     1081 ggccgctagc tttaatgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     1141 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1201 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1261 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1321 tgcccgcctg atgaatgctc atccggagtt ccgtatggca atgaaagacg gtgagctggt
     1381 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     1441 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     1501 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     1561 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     1621 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     1681 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     1741 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aattggtacg
     1801 tcga
//
