LOCUS       V004210                 6598 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004210
VERSION     V004210
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Papadimitriou K, Anastasiou R, Maistrou E, Plakas T, Papandreou NC,
            Hamodrakas SJ, Ferreira S, Supply P, Renault P, Pot B, Tsakalidou E.
  TITLE     Analysis of the lactococcal plasmid pSMA198 found in Streptococcus
            macedonicus ACA-DC 198 supports the dairy origin of the species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Papadimitriou K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-APR-2014) Papadimitriou K., Food Science and Human
            Nutrition, Agricultural University of Athens, 75 Iera Odos str.,
            Athens, 117 55, GREECE
REFERENCE   3  (bases 1 to 6598)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6598)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-APR-2014) Papadimitriou K., Food Science and Human Nutrition,
            Agricultural University of Athens, 75 Iera Odos str., Athens, 117
            55, GREECE"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      559..800
                     /label="predicted ori of plasmid pSMA198"
                     /note="predicted ori of plasmid pSMA198"
     CDS             833..2026
                     /codon_start=1
                     /gene="rep"
                     /product="Rep protein"
                     /label="rep"
                     /note="replication initiation protein of plasmid pSMA198"
                     /protein_id="CDP22284.1"
                     /translation="MSSIPEKQENQKQVLTLNELSKRKVVEHNSLITSIAKMDKTPLKM
                     FELAVSCINTEEPPKDNIVYLSKRDLFAFFKVSDNDKHSRFKEAIEKMQKQAFFQIKEE
                     AGKGFKFKSIVPIPYVEWTDYHDEVKIEFHREIMPYLINLKKNFTQHALSDLAELNSKY
                     SIILYRWLSMNYNQYEHYSAKGGRRAEQVESYQNPSISIKELRIMTDTVNDYERFQSLE
                     TWILKKPLEEINAHTSFNVTYDKIKKGRSIDSIVFHIEKKRCADDNSYKLNDKMYQAEQ
                     AKKEETEDMLAIEAMKNKYTKLLLDNMLLNSYEMTDTAIMAGLQQHVYPLYDELKDLHG
                     IKAVQDHLSYVASKQEAYSKRNIAKYLKKAIEQYLPTVRGSEIENMADLLKKIEGKA"
     gene            833..2026
                     /gene="rep"
                     /label="rep"
     CDS             2023..2607
                     /codon_start=1
                     /product="OrfX"
                     /label="OrfX"
                     /note="ORFX; orfX of plasmid pSMA198"
                     /protein_id="CDP22285.1"
                     /translation="MSEDLKTIKELADEFNVSKQAVRKRLTEEFRANHVETVTSNGVKT
                     LVVTYTGYMLLKQHFTTSNATGNDKVTDTSNPTTGDLSVVKILEQQLFVKDKQLENKDS
                     QISQMQNLLDQQQRLALQDKKLLEEYKAENDSLKALKMPSQETEFKHLDNQYKDEVNAL
                     KEKLENLQEQLKEKDQELAQEKSKKWYQFWK"
     CDS             3319..4053
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     primer_bind     complement(4140..4156)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4164..4180)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4188..4218)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4233..4254)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4542..5130)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5304..6161)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6162..6266)
                     /label="AmpR promoter"
ORIGIN
        1 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
      181 gatcatggac gagccactcg tatctggttg gatttaattc ttaaaaataa actacacaaa
      241 atcgtcgatt ggaatcaaat tgataaggat gaatatttaa atgccatgat ccgtagtaca
      301 gtcagcacca atgaattaaa atatttgatt caaaaggcat taaccgatga tttaggaaaa
      361 gaacaattct ttaaaggaat tgatgcgagt tattattacg agggctatta cgaaattaaa
      421 acagaagatc tataaacacg tataaacacg atttaaagaa acagactgct caatataaaa
      481 cttgagcagt ctgtttcttt ttttggatga ttttgacctt tggttttaaa tttttgaaaa
      541 aaataaaaaa taggcgaagc ctattatata tttatcttat atattttaat cttttattct
      601 tttgcgtgcc caaaaagtca gcaatagtaa ggctatgcag atttttatag caagaaaaaa
      661 ctgtgtatat agcaagaaaa aactgtgtat atagcaagaa aaaactgtgt atatagcaag
      721 aaaaaactag ataaccttgc tatattatga tataataaaa gcatagagaa aattcacgac
      781 gaaatgactt ctctatgcct acttaaaatc actcacaaag gagtaacttt ctatgtctag
      841 tataccagaa aaacaagaaa atcaaaagca ggtgctgacc ttgaatgaac tgtcaaaaag
      901 aaaagtggta gagcataaca gcttgattac gtctattgct aaaatggata aaaccccact
      961 gaaaatgttt gagttagcgg tgtcttgtat caataccgaa gagccaccaa aagacaatat
     1021 tgtttatctc tcaaagagag accttttcgc tttctttaaa gtatccgata atgacaagca
     1081 tagtcgcttt aaagaagcga ttgagaaaat gcaaaaacaa gccttttttc aaatcaaaga
     1141 agaagcaggc aaaggtttta aatttaaaag cattgttcct ataccttacg tggaatggac
     1201 agattatcat gatgaagtaa aaattgaatt tcatcgtgaa attatgcctt acttaatcaa
     1261 tcttaaaaag aattttacac aacatgcttt gtctgactta gcagaattaa atagcaagta
     1321 cagcattatc ttatatcgtt ggttatccat gaattataac cagtacgagc attacagcgc
     1381 taaaggcgga cggcgagccg aacaagtaga aagctaccaa aatccgtcta tttcaattaa
     1441 agaattgcgt atcatgactg atactgtaaa tgattatgaa cggtttcaga gtttagaaac
     1501 atggatatta aaaaaaccgt tggaagaaat caacgcccat acgtctttta acgtgactta
     1561 tgacaaaatc aaaaaaggac ggagcattga ctctattgtc tttcatatcg agaagaaacg
     1621 gtgtgcagac gataatagct acaaactcaa tgataaaatg tatcaagcag aacaagcaaa
     1681 gaaagaagaa acagaagata tgctagctat tgaagcaatg aaaaataaat atactaagct
     1741 attactcgat aatatgcttc taaattctta tgaaatgaca gatacagcaa ttatggcagg
     1801 cttacagcaa catgtttatc ctctctatga tgagttgaaa gacttacatg gaataaaagc
     1861 agttcaagac catctctcat atgttgctag taagcaagaa gcttattcaa aacggaatat
     1921 tgctaaatac cttaaaaaag cgattgaaca atacctccca acagttagag gaagtgagat
     1981 agaaaatatg gcagacctat tgaaaaaaat agagggtaaa gcatgagtga agatttaaaa
     2041 accataaaag aacttgcaga cgaatttaac gtatcgaaac aagctgtcag gaaaagatta
     2101 accgaagaat ttagagcaaa ccatgtggaa accgttacta gtaacggagt gaaaacttta
     2161 gtagtaacct atactggata tatgttgtta aaacaacact ttaccactag taacgcaact
     2221 ggtaacgaca aggtaaccga tactagtaac cccacaactg gtgatttatc tgtagttaaa
     2281 atactagaac aacagttatt tgttaaagat aagcaactag aaaataaaga tagccaaata
     2341 tcgcaaatgc aaaatctctt agaccaacaa caacgcctag ctttgcagga taaaaagcta
     2401 ctggaagaat acaaggcgga aaatgacagt ctaaaagccc tcaaaatgcc ctcacaggaa
     2461 acagaattca aacacttaga caatcaatat aaagatgaag tgaacgctct taaagagaag
     2521 ttggaaaatt tgcaggaaca gctgaaagaa aaagaccaag aattagcaca agaaaaatct
     2581 aaaaaatggt atcaattttg gaaatagaaa gtcttttaaa aagatagaac agtctaaagc
     2641 ataagaacat tatagtgaaa tattctttgg atcgtttcta aaatcaacta ctagccccat
     2701 ggggaatatc taacaatttt tgccaaaatt tatccagttt gtttaaaaaa aattgattat
     2761 gatagtatca agaagaagag agggagaaat agatgaatgg atcctctaga gtcgacctgc
     2821 agacgtatat agatttcata aagtctaaca cactagactt atttacttcg taattaagtc
     2881 gttaaaccgt gtgctctacg accaaaacta taaaaccttt aagaactttc tttttttaca
     2941 agaaaaaaga aattagataa atctctcata tcttttattc aataatcgca tccgattgca
     3001 gtataaattt aacgatcact catcatgttc atatttatca ggatcctcta gagtcacaaa
     3061 aagaaaaacg aaatgataca ccaatcagtg caaaaaaaga tataatggga gataagacgg
     3121 ttcgtgttcg tgctgacttg caccatatca taaaaatcga aacagcaaag aatggcggaa
     3181 acgtaaaaga agttatggaa ataagactta gaagcaaact taagagtgtg ttgatagtgc
     3241 agtatcttaa aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa
     3301 ttaagaagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
     3361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
     3421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
     3481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat
     3541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
     3601 tataaaattg ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt
     3661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
     3721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
     3781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
     3841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
     3901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
     3961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     4021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     4081 tggaaagtta cacgttacta aagggaatgc tgcaggcatg caagcttggc gtaatcatgg
     4141 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4201 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4261 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4321 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4381 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4441 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4501 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4561 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4621 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4681 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4741 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4801 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4861 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4921 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4981 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5041 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5221 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5281 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5341 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5401 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5461 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5521 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5581 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5641 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5701 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5761 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5821 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5881 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5941 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6001 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6061 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6241 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     6301 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     6361 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6421 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6481 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     6541 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggc
//
