LOCUS       40924_33752        7541 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Inactivation vector pOKvtpKO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 7541)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky Mountain 
            Laboratories, National Institute of Allergy and Infectious Diseases,
            National Institutes of Health, 903 S. 4th Street, Hamilton, MT 
            59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(51..595)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             805..1611
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(1727..2512)
                     /note="similar to thymidylate synthase; 3' end of gene is
                     not cloned into the vector"
     gene            1727..2512
                     /gene="thyX"
                     /label=thyX
                     /note="orfZ"
     CDS             complement(2796..3686)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orfY"
                     /protein_id="ABS71244.1"
                     /translation="MKKSILTVCMFILLCLLSCDIDALNDLLSKAREKFLEENKNIEGS
                     DHKQEGKEEQVDVVKNMEEGVRQVVQGDPVAPVDDAIPAFQYPQQETIEIEEKDLIPST
                     KEEKEAQEEIEKVKSVLEDSKFDQLIENSRKLQSESKQLESDFYRIFSELQTKLQEQRS
                     LPKINSQTDRAKIQELIKLQNRFNEKRTQIDMLMTQVDAGFNERSSAKYFFEEAEKTLK
                     EAITERLKNALRSSFRRVANYLSGQLSREARRYAENSLSLLESSSGKIGEAMGIRKDIE
                     ELIKEAKSYLSSLVR"
     regulatory      4175..4295
                     /label=flgB promoter derived from Borrelia hermsii
                     /note="flgB promoter derived from Borrelia hermsii"
                     /regulatory_class="promoter"
     CDS             4296..5102
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             6073..6603
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orfX"
                     /protein_id="ABS71246.1"
                     /translation="MSFSFKNFKLTKMLFIFLLAACSSLQVEHDKINNKVRIYQYLNEN
                     FELKGVVDHQNNTTQIFLYTKIRNHSIVKQTPLILPDGTKIEGKTSYEYDSKASLGKWV
                     NASSFSLNKAILKKILNEDEHVYNKEDIKVQVGLETLKINKAKIIEFLSKLNAIEKQHT
                     QKQHPDKQNTHKQ"
ORIGIN
        1 gcgtattgga gctttcgcga gggaagtgag agggccgcgg caaagccgtt tttccatagg
       61 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
      121 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
      181 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
      241 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
      301 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
      361 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtttgaa
      421 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
      481 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
      541 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
      601 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcctagagtc gagggggggg ggggaaagcc acgttgtgtc tcaaaatctc
      721 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      781 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
      841 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      901 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
      961 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1021 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1081 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1141 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1201 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1261 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1321 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1381 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1441 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1501 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     1561 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     1621 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     1681 tcattcgcca ttcaggctgc gcaactgttg ggaagggcga ggcgcgcctc tttaatgcct
     1741 tatcacttaa ttttagttta ctggtatcca aaactccaga aattgcaact atctcttcat
     1801 aagataatct acaccctttt aagatatgat ttttgaaact tttagtagca ataggcacaa
     1861 tcttttcaat aatatcaagc aatatcttgg catattcgcg catttccttt gatgaatgtt
     1921 ctctaacctc ttttgactca tttaaagcta atcttaattt tataaaatga aaaagattat
     1981 ttaaatcaat ctgccaatac cattcggtat ataaacttaa gggcaaaact attctagaga
     2041 cttcctttga aacattataa tctatcatat tttgatatag ctcgtaacaa gaactttgaa
     2101 tttcctttaa attacttatt atatcctttg taaagttata tgtaattcct tcatcctcac
     2161 cttgactatt ctcaatattt tgaattttca tgtcttcttc taaaggcaca tagaactctt
     2221 ccctcatcaa actataagaa ccagaaactt cattcatcct tgctgttcta tgtctcatcc
     2281 attgtcttgc aataaatatg ggagccttaa catgaaatgc aaacactact tgttcaaatg
     2341 gacttgtatg ttcatttctg attaaataat cgataagctc attatcttcc ctcttaatgc
     2401 tctcacttcg atatgaaatc cttgctgctt gaactattct tgcgtcacta cccatatagt
     2461 caataagctt tataaagcct ttatccaaaa ccttatattc ctgatttaac aagccctctt
     2521 tttcgatctc agtattcaaa catgtacccc cactttttat ttattcgtta ctcaatttac
     2581 attattataa caaagctatt taacaccttt caaaaacaat ttggaacaat aatccttata
     2641 cttacaaacc cttaagatat ggtaataaaa aatgcatagt caattacaaa ggtaagcatt
     2701 tttagaaact tatttaaaaa attaaagaga gccccctata aaggaactct tcttgcaaat
     2761 actaatatta taaaatattt aaacctttgg tatctctatc ttacaagact tgatagataa
     2821 gatttagctt ctttaatgag ttcctctata tctttcctta ttcccattgc ttctcctatt
     2881 ttcccagaag aagattctaa taaacttaaa gaattttctg catatcgtcg tgcttctctg
     2941 gataattgtc ctgataaata attagctact cttctgaaag aggaccgtaa tgcatttttt
     3001 aatctttcag taatggcttc ttttaaagtt ttttcagctt cttcaaaaaa gtattttgcg
     3061 gaacttcttt cattaaagcc tgcatcaact tgggtcatca acatatcgat ctgagtcctt
     3121 ttttcattga acctattttg caacttaatt agttcttgta tctttgctct atctgtttga
     3181 ctattgattt taggaagaga cctttgctct tgaagtttcg tctgaagttc tgagaatatt
     3241 ctataaaaat cggattccaa ttgcttagac tctgattgaa gcttgcgtga gttctcaatt
     3301 aattgatcaa atttggaatc ttcaaggaca ctttttacct tttcaatttc ttcttgtgct
     3361 tccttttctt ctttagtgct tggaattaaa tctttttctt ctatttctat agtttcttgt
     3421 tgaggatatt gaaatgccgg aatagcatca tcaacaggtg ctacaggatc gccctgaact
     3481 acttgacgta ctccttcttc catattttta acaacatcta cttgctcttc tttaccttcc
     3541 tgtttatggt ctgaaccttc tatattttta ttttcttcta aaaatttttc ccttgctttg
     3601 cttagcaaat cattaagggc atctatatca catgataaca aacacaataa tatgaacata
     3661 catactgtta aaatactttt tttcatattt ccctcctgag atatcaaatt actcaattat
     3721 tgagtctcaa tattgaaata caataattac taacaaatat ataccattta atttaaatta
     3781 caaaaataaa ttctgtagaa tagtgagatt attttactct gtgccactta aaagcagtac
     3841 aaagttattt tactaagcaa acttatacaa taaaaaatat aatcttaatc taaacgctgg
     3901 gaaagtaaat aaagtaaaga taaaagatgg tcttaaataa tatcagaagc taagagcata
     3961 gtgctcttag ctttctattt attgacttta tttttccagt tacttacatt ttgattaaaa
     4021 tttaatctta taataatatt taaaattata tataccttag ttaaggttta acaggggtcg
     4081 ctggcttagc aggagcagct gtaagctccg caattgcagc ttctactgca tctttagcag
     4141 cggttaagta tctgcagaat tcgcccttgc cggcgttaaa gaaaattgaa ataaacttgg
     4201 actatgttaa tgaaaaagta aaaattaacg aacaaaaaga tttaaattat tatataatat
     4261 agtaaaaagc aatttgtgat atagagggtg ttcatatgag ccatattcaa cgggaaacgt
     4321 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     4381 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     4441 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     4501 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     4561 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     4621 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     4681 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     4741 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     4801 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     4861 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     4921 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     4981 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     5041 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     5101 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     5161 acggcgctag ctcattcgcc aagccgaatt ccagcacact ggcggccgtt actagtcacc
     5221 ctttttaagc tctgggcctc cattattaca agatataaat aaaaataaag tcatcaatat
     5281 cgcacttaat gtattcttct tcattattat gtgcctcctt attaacatac attaatagtg
     5341 caaaaatttt acttttcaag tttttaaaaa aaattataaa aaaattcgta aataatttta
     5401 caaaacaaaa atattatcaa tatctataca aatctatata aataattcaa gcaaaaaatg
     5461 taactaaaca tcaataaaac tcaataaaat tacactcaat ttgaataata cattcaactc
     5521 acatcccaaa caactttgtt agctgcacaa agaattaatt acaaatatgt gttaaatctt
     5581 ataatctcac ggcacatgag aataatggca ggataaaatt aataaaatct atatagaact
     5641 atatattatt taaaataaag caaagcactt acatacattt acatggcaat atcaccagct
     5701 actaaaacat caaataaatt tgatactact taaataatat aatactttac ataacggtaa
     5761 gtttaaatac ataaatagac agccatattt caaaatattt atgatacaat ctgtgactaa
     5821 atatactatt ttcaagtaat atcctaagtc gatgaacaga taaattaata ctttaaagga
     5881 aagaataatt tctctgtaat taaattaaac acaattataa attcattaaa gtttaattta
     5941 agattattaa tcttgttttt caaatttatt aaagtataat gtttaaaaga tatcataatg
     6001 attatttaca aaaactataa atgatgcata aataatatac tatatattaa catagtatta
     6061 aaaggagggg atatgtcatt ttctttcaag aattttaaat taacaaaaat gttattcata
     6121 tttttacttg cagcatgttc ttccttgcaa gttgaacatg ataaaatcaa taacaaggta
     6181 agaatatatc aatatttaaa cgaaaacttc gaattaaaag gcgtggttga tcatcaaaat
     6241 aacacaacgc aaatattttt atatactaaa ataagaaatc acagtatagt taaacaaact
     6301 ccattaatac taccagatgg cactaaaata gaaggtaaaa caagctatga atatgatagt
     6361 aaagcttcgc ttggcaaatg ggttaacgcc tcctctttta gcttaaacaa agccatatta
     6421 aagaaaatac taaatgaaga tgagcatgta tataacaagg aagacattaa agttcaagtc
     6481 ggcctagaaa cattgaaaat caataaagcc aaaattatag aattcctatc aaaattgaac
     6541 gcaattgaaa agcaacatac ccaaaagcaa catcccgaca aacaaaatac tcacaaacaa
     6601 taatcaaagc tttaaaatta acgatacatc cctaaacgtt agttttaaag cttttttatt
     6661 atccttatca acttatcaac taataagctt atataaccac ttgctcataa aagcatcacc
     6721 tttaaagtga aatcactata gattttaaaa caaatttttc aaaagaaaca agaaaattta
     6781 atacaaaaac taatttatta attaaagaat aaacatataa aatataagct atacaataac
     6841 accatattta aacccaacac tatatataga atggaaggcg aaaacaagat aatgacctgc
     6901 ataagaaatt taaaatctac atatttaaac taaattagca taaaatgaca aaggctacct
     6961 accgtagcca acctattatt ctaatcaaac aataagagtg agacttcaaa tctcagtcta
     7021 taggataatt ataaactatt ttattttttt gtaaagtctt ttttgaaagt ttttatgcta
     7081 tttaaaaaat attctcaaga acctattatc aaaacttgat aagttatcaa accaatataa
     7141 atacccaatt aagacacaat ggggttattg tacttttaca gtattatatc tatcgcgcat
     7201 cttattaaaa aaatcttttt cattcttgta tatttcttta agcaaaaagc tagcaaattt
     7261 tgtattggat ttgtaaaaat gaaaactctc ttcatcatct agttgaaacc ttaaaggtct
     7321 tagagggttg acattgcttt tactaatgat attagtattt tccagcacct taattgcata
     7381 atctacccct ctacttaaaa tcaaatcata atcaatctta ccgctatcaa ttcctgtaac
     7441 taacttaata tacctatata tggaagtttt tgctatttta tagctcttga taaaagctgt
     7501 aaaactttta tatccatcaa gagcatagta tttgccctag g
//
