LOCUS       40924_33747        5165 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pOKE104, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Low W, Jedd G.
  TITLE     An improved plasmid for transformation of Neurospora crassa using 
            the pan-2 gene as a selectable marker
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Low W, Jedd G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2008) Temasek Life Sciences Laboratory, 1 Research
            Link National University of Singapore 117604, Singapore
REFERENCE   3  (bases 1 to 5165)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-DEC-2008) Temasek Life Sciences Laboratory, 1 Research Link 
            National University of Singapore 117604, Singapore"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..61
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(68..86)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(104..120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(128..144)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(152..182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(197..218)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(506..1094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1268..2125)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2126..2230)
                     /label=AmpR promoter
     CDS             2889..2912
                     /codon_start=1
                     /label=8xHis
                     /note="8xHis affinity tag"
                     /translation="HHHHHHHH"
     CDS             join(2989..3255,3473..4135)
                     /codon_start=1
                     /gene="pan-2"
                     /product="3-methyl-2-oxobutanoate hydroxymethyltransferase"
                     /label=pan-2
                     /note="pantothenic acid-2"
                     /protein_id="ACM43517.1"
                     /translation="MGLPPTNPRKKVTLNTLQSMYKKGEKITMITAHDFPSAHVAEHAG
                     MDMILVGDSLAMVALGMEDTSEVLVEEMLLHCRSVARATSRAFTVGDLPMGSYEISPQQ
                     ALSTAIRFIKEGRVSAVKLEGGAEMAPTIRAITTAGIPVLAHIGLTPQRQNALGGFRVQ
                     GKSKESAKRLLRDALAVQEAGAFAVVVEAVPQEVAAFITGLLKIPTIGIGAGNGTSGQV
                     LVQADMTGNFPPGRFLPKFVKKYGDVWGEALRAIETYRDEVKGGSYPAEEHTYPMAKEE
                     VEAFVGDVRRLMNANASAKAEGEGEKDA"
     gene            2989..4135
                     /gene="pan-2"
                     /label=pan-2
     rep_origin      4530..4985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5126..5142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5149..5165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
       61 tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
      121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      481 gtaaaaaggc cgcgttgctg gcgtttttcg ataggctccg cccccctgac gagcatcaca
      541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
      961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1561 gcaacgttgt tggcattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga ataccgcgcc cggcgaccga
     1861 gttgctcttg cccggcgtca atacgggata atagtgtatg acatagcaga actttaaaag
     1921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgtcgacagt caccgatgcc
     2281 tcggccccag ggacacagcc aatcaagcac atccaagcga ccggtaaagt gggtagtgtg
     2341 ggtctttctc cgaacgcccg aacaagccgg cgggtcccag tccccccctt gacacttggc
     2401 ggaaacgggc aaagaacacc aagaaatttg tgagatatac tcaattctac gcagcaaaaa
     2461 ccatcatggc ttcaggagtt gaaataaaaa ggaggagccg ggcgcagtga gaaaaaaaaa
     2521 atggcgcaaa tgtagaaatc atttactctt cctctcaatc aatcactttc agtgcagcat
     2581 tggttatttc catcaaccac caaaaccgtg gggaaaagtt gcatcgattg ttccaagcat
     2641 cgcaaagtcg cttcattcat tccgttgtac cttcaccttg cgtatattct ggaccggtac
     2701 tttacatacg cttctcatca cttatataac accaccacca cagccacaac cattacaacc
     2761 accataatgc acaagcaaat aacatcttca ctactccgga ggtcttctcc cttcctttcc
     2821 aggggtacca attgcaaacc attagtcctg ctgcgtggtg ccggcggtgc atctcaacct
     2881 cacctcaaca ccaccaccac caccaccacc acgggatact accaacccca actacaccac
     2941 caccaccaac atgtgcaaat ccaaacccgc tattcttctc actcccccat gggcctcccg
     3001 cccaccaacc cccgcaaaaa ggtcacgctc aacacgctgc agtccatgta caaaaagggc
     3061 gagaaaatca ccatgatcac ggcgcacgat ttcccttcgg cacacgtggc cgagcacgcg
     3121 ggcatggaca tgatcctggt gggcgacagc ctggccatgg ttgctctggg gatggaggac
     3181 acgagcgagg tgttggtgga ggagatgttg ctgcattgtc ggagtgtggc gagggctact
     3241 agccgggctt ttactgtgag tttttcttct tcttctaata tcctatatcc tctcaactca
     3301 tggtctctat ttgagaaccc tttgtttata cctaccccta tttttttcct tctccgtcgt
     3361 ctcatttatt tttccctcgt cttcaatcat tgcccctatt cactataccc tactccctat
     3421 gtgaaccatt agaaaaaaga aagaaaaaaa aaaacactaa ccatgcttcc aggtcggcga
     3481 cctccccatg ggctcctacg aaatctcccc ccaacaagcc ctctccaccg ccatccgctt
     3541 catcaaagaa ggccgcgtgt ccgccgtcaa actcgaaggc ggcgccgaga tggcccccac
     3601 catccgcgcc atcaccaccg ccggcatccc cgtgctcgcc cacatcgggc tcactcccca
     3661 gcgccaaaac gccctgggtg gcttccgcgt ccagggcaaa tccaaagaat ccgccaagcg
     3721 attactccga gacgccctgg ccgtccagga ggccggtgct ttcgccgtgg tggtggaggc
     3781 cgtgccgcag gaggtggcgg ccttcatcac cggtctcctg aaaatcccaa ccattggcat
     3841 tggtgctgga aacggcacga gtggccaggt gctggtgcag gccgatatga cggggaattt
     3901 cccgcctggg aggttcttgc ccaagtttgt gaaaaagtat ggcgatgtgt ggggggaggc
     3961 gttgagggct attgagacgt atagggatga agtcaagggc gggagttatc cggccgagga
     4021 gcatacgtac ccgatggcga aggaggaggt ggaggccttt gtgggggatg tgaggaggct
     4081 gatgaatgcg aatgcgtctg ccaaggctga aggggagggt gagaaggatg cgtgagtaat
     4141 gctgtagtgt gtgcgtggca gcggcgggag gaggaggata atacgccttg catgatgttg
     4201 ggataaaaaa atgtgttgct gtttttggat ataagagcgt tgcctcacgt tgatagtgta
     4261 gcaatttgat atgtacctct gtcgtgatcc ctttaatttg atgtgagatg ccgtggatat
     4321 tgatgacctt gacggtgtgg cgttccgtgg tgtcaaaacc cttcagatag ttgctcaagg
     4381 atgctgaagt ttccatagta atcctagatt tgttggatga tttttcgaca cggctcgtca
     4441 cagtcttgac acagtctaac atgctgtcca taacatatgc ggtgtgaaat accgcacaga
     4501 tgcgtaagga gaaaataccg catcaggcga cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag agctttacgg cacctcgacc gcaaaaaact
     4741 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4861 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4921 aaaaaatgag ctgatttaac aaatatttaa cgcgaatttt aacaaaatat taacgtttac
     4981 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     5041 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     5101 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
     5161 ctata
//
