LOCUS       40924_33742        7530 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pOK12-vtp-gentR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     A System for Site-Specific Genetic Manipulation of the Relapsing 
            Fever Spirochete Borrelia hermsii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Battisti JM, Raffel SJ, Schwan TG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky 
            Mountain Laboratories, National Institute of Allergy and Infectious 
            Diseases, National Institutes of Health, 903 S. 4th Street, 
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 7530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2007) Laboratory of Zoonotic Pathogens, Rocky Mountain 
            Laboratories, National Institute of Allergy and Infectious Diseases,
            National Institutes of Health, 903 S. 4th Street, Hamilton, MT 
            59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(51..595)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             805..1611
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(1727..2512)
                     /gene="thyX"
                     /note="similar to thymidylate synthase; orfZ; 3' end of
                     gene is not cloned into the vector"
     gene            complement(1727..2512)
                     /gene="thyX"
                     /label=thyX
     CDS             complement(2796..3686)
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orfY"
                     /protein_id="ABS71239.1"
                     /translation="MKKSILTVCMFILLCLLSCDIDALNDLLSKAREKFLEENKNIEGS
                     DHKQEGKEEQVDVVKNMEEGVRQVVQGDPVAPVDDAIPAFQYPQQETIEIEEKDLIPST
                     KEEKEAQEEIEKVKSVLEDSKFDQLIENSRKLQSESKQLESDFYRIFSELQTKLQEQRS
                     LPKINSQTDRAKIQELIKLQNRFNEKRTQIDMLMTQVDAGFNERSSAKYFFEEAEKTLK
                     EAITERLKNALRSSFRRVANYLSGQLSREARRYAENSLSLLESSSGKIGEAMGIRKDIE
                     ELIKEAKSYLSSLVR"
     CDS             complement(4061..4690)
                     /codon_start=1
                     /gene="vtp"
                     /product="variable tick protein"
                     /label=vtp
                     /note="derived from Borrelia hermsii"
                     /protein_id="ABS71240.1"
                     /translation="MKKNTLSAILMTLFLFISCNNGGPELKKGEVTKSDGTVLDLSKIS
                     ANIKNAVTFAASVQEVETLVKSIDELAKAIGQKVNADGLTAEADKNDSLVAGVYQLISD
                     VQGKLTKLEIGASKFAGLKEKVVAAKKGSDDFLTKVKAQHNNLGQSAEAPKAIKKGNAD
                     STKGAEELGKLNTAIDELLTAAKDAVEAAIAELTAAPAKPATPVKP"
     gene            complement(4061..4690)
                     /gene="vtp"
                     /label=vtp
     regulatory      4984..5269
                     /label=flaB promoter derived from Borrelia hermsii
                     /note="flaB promoter derived from Borrelia hermsii"
                     /regulatory_class="promoter"
     CDS             5270..5800
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     CDS             6063..6593
                     /codon_start=1
                     /product="hypothetical protein"
                     /label=hypothetical protein
                     /note="orfX"
                     /protein_id="ABS71242.1"
                     /translation="MSFSFKNFKLTKMLFIFLLAACSSLQVEHDKINNKVRIYQYLNEN
                     FELKGVVDHQNNTTQIFLYTKIRNHSIVKQTPLILPDGTKIEGKTSYEYDSKASLGKWV
                     NASSFNLNKAILKKILNEDEHVYNKEDIKVQVGLETLKINKAKIIEFLSKLNAIEKQHT
                     QKQHPDKQNTHKQ"
ORIGIN
        1 gcgtattgga gctttcgcga gggaagtgag aggtccgcgg caaagccgtt tttccatagg
       61 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
      121 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
      181 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
      241 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
      301 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
      361 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtttgaa
      421 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
      481 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
      541 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
      601 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcctagagtc gagggggggg ggggaaagcc acgttgtgtc tcaaaatctc
      721 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
      781 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
      841 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      901 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
      961 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1021 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1081 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1141 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1201 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1261 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1321 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1381 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1441 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     1501 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     1561 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     1621 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     1681 tcattcgcca ttcaggctgc gcaactgttg ggaagggcga ggcgcgcctc tttaatgcct
     1741 tatcacttaa ttttagttta ctggtatcca aaactccaga aattgcaact atctcttcat
     1801 aagataatct acaccctttt aagatatgat ttttgaaact tttagtagca ataggcacaa
     1861 tcttttcaat aatatcaagc aatatcttgg catattcgcg catttccttt gatgaatgtt
     1921 ctctaacctc ttttgactca tttaaagcta atcttaattt tataaaatga aaaagattat
     1981 ttaaatcaat ctgccaatac cattcggtat ataaacttaa gggcaaaact attctagaga
     2041 cttcctttga aacattataa tctatcatat tttgatatag ctcgtaacaa gaactttgaa
     2101 tttcctttaa attacttatt atatcctttg taaagttata tgtaattcct tcatcctcac
     2161 cttgactatt ctcaatattt tgaattttca tgtcttcttc taaaggcaca tagaactctt
     2221 ccctcatcaa actataagaa ccagaaactt cattcatcct tgctgttcta tgtctcatcc
     2281 attgtcttgc aataaatatg ggagccttaa catgaaatgc aaacactact tgttcaaatg
     2341 gacttgtatg ttcatttctg attaaataat cgataagctc attatcttcc ctcttaatgc
     2401 tctcacttcg atatgaaatc cttgctgctt gaactattct tgcgtcacta cccatatagt
     2461 caataagctt tataaagcct ttatccaaaa ccttatattc ctgatttaac aagccctctt
     2521 tttcgatctc agtattcaaa catgtacccc cactttttat ttattcgtta ctcaatttac
     2581 attattataa caaagctatt taacaccttt caaaaacaat ttggaacaat aatccttata
     2641 cttacaaacc cttaagatat ggtaataaaa aatgcatagt caattacaaa ggtaagcatt
     2701 tttagaaact tatttaaaaa attaaagaga gccccctata aaggaactct tcttgcaaat
     2761 actaatatta taaaatattt aaacctttgg tatctctatc ttacaagact tgatagataa
     2821 gatttagctt ctttaatgag ttcctctata tctttcctta tccccattgc ttctcctatt
     2881 ttcccagaag aagattctaa taaacttaaa gaattttctg catatcgtcg tgcttctctg
     2941 gataattgtc ctgataaata attagctact cttctgaaag aggaccgtaa tgcatttttt
     3001 aatctttcag taatggcttc ttttaaagtt ttttcagctt cttcaaaaaa gtattttgcg
     3061 gaacttcttt cattaaagcc tgcatcaact tgggtcatca acatatcgat ctgagtcctt
     3121 ttttcattga acctattttg caacttaatt agttcttgta tctttgctct atctgtttga
     3181 ctattgattt taggaagaga cctttgctct tgaagtttcg tctgaagttc tgagaatatt
     3241 ctataaaaat cggattccaa ttgcttagac tctgattgaa gcttgcgtga gttctcaatt
     3301 aattgatcaa atttggaatc ttcaaggaca ctttttacct tttcaatttc ttcttgtgct
     3361 tccttttctt ctttagtgct tggaattaaa tctttttctt ctatttctat agtttcttgt
     3421 tgaggatatt gaaatgccgg aatagcatca tcaacaggtg ctacaggatc gccctgaact
     3481 acttgacgta ctccttcttc catattttta acaacatcta cttgctcttc tttaccttcc
     3541 tgtttatggt ctgaaccttc tatattttta ttttcttcta aaaatttttc ccttgctttg
     3601 cttagcaaat cattaagggc atctatatca catgataaca aacacaataa tatgaacata
     3661 catactgtta aaatactttt tttcatattt ccctactgag atatcaaatt actcaattat
     3721 tgagtctcaa tattgaaata caataattac taacaaatat acaccattta atttaaatta
     3781 caaaaataaa ttctgtagaa tagtgagatt attttactct gtgccactta aaagcagtac
     3841 aaagttattt tactaagcaa acttatacaa taaaaaatat aatcctaatc taaacgctgg
     3901 gaaagtaaat aaagtaaaga taaaagatgg tcttaaataa tatcagaagc taagagcata
     3961 gtgcccttag ctttctattt attgacttta tttttccagt tacttacatt ttgattaaaa
     4021 tttaatctta taataatatt taaaattata tataccttag ttaaggttta acaggggtcg
     4081 ctggcttagc aggagcagct gtaagctccg caattgcagc ttctactgca tctttagcag
     4141 cggttaacaa ctcatctatt gctgtattta acttaccaag ttcttcagct cccttagtac
     4201 tatcagcatt acctttcttt atagcctttg gggcttcagc actttgacca agattattat
     4261 gctgcgcttt tactttggtt aagaaatcat cacttccctt cttagcagca acaacctttt
     4321 cctttagtcc agcaaacttg ctggccccaa tttccaattt tgtcaactta ccctgtacat
     4381 ctgatattaa ttgatatacc cctgcaacca atgaatcatt tttatctgct tcagcagtaa
     4441 gaccatctgc attaactttc tgtccaatag ctttagcaag ctcatcaata gacttaacta
     4501 aggtctctac ttcttgaaca cttgcagcaa aagtaacagc gttttttata tttgcactta
     4561 tttttgataa atcaagaact gttccatcag atttagttac ttcacccttt ttaagctctg
     4621 ggcctccatt attacaagat ataaataaaa ataaagtcat caatatcgca cttaatgtat
     4681 tcttcttcat tattatgtgc ctccttatta acatacatta atagtgcaaa aattttactt
     4741 ttcaagtttt taaaaaaaat tataaaaaaa ttcgtaaata attttacaaa acaaaaatat
     4801 tatcaatatc tatacaaatc tatataaata attcaagcaa aaaatgtaac taaacatcaa
     4861 taaaactcaa taaaattaca ctcaatttga ataatacatt caactcacat cccaaacaac
     4921 tttgttagct gcacaaagaa ttaattacaa atatgtgtta aatcttataa tccccgggcc
     4981 ggcaattcct aatcagaaaa atgtggttga agattataga aagaagtatg aggaaaggga
     5041 gaataaggtt gaaggagaac ttaatacttt ggatttcact gtaaagcgga tgaaggaaca
     5101 agaggaaatc cttaaatctt tgaatttaca gcgacaaaac aggtaacaaa tttaatttaa
     5161 attaatattg tatttatttt tttcttgttt ttgtgatatc cttttaaaaa gacaaatgga
     5221 tttgtctaaa tattaaaatt atattaattt atcacggagg aaatgacata tgttacgcag
     5281 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
     5341 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
     5401 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
     5461 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
     5521 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag
     5581 tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg gcattgccac
     5641 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
     5701 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
     5761 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
     5821 gactagtaat accctaagtc gatgaacaga taaattaata ctttaaagga aagaataatt
     5881 tctctgtaat taaattaaac acaattataa attcattaaa gtttaattta agattattaa
     5941 tcttgttttt caaatttatt aaagtataat gtttaaaaga tatcataatg attatttaca
     6001 aaaactataa atgatgcata aataatatac tatatattaa catagtatta aaaggagggg
     6061 atatgtcatt ttctttcaag aattttaaat taacaaaaat gttattcata tttttacttg
     6121 cagcatgttc ttccttgcaa gttgaacatg ataaaatcaa taacaaggta agaatatatc
     6181 aatatttaaa cgaaaacttc gaattaaaag gcgtggttga tcatcaaaat aacacaacgc
     6241 aaatattttt atatactaaa ataagaaatc acagtatagt taaacaaact ccattaatac
     6301 taccagatgg cactaaaata gaaggtaaaa caagctatga atatgatagt aaagcttcgc
     6361 ttggcaaatg ggttaacgcc tcctctttta acttaaacaa agccatatta aagaaaatac
     6421 taaatgaaga tgagcatgta tataacaagg aagacattaa agttcaagtc ggcctagaaa
     6481 cattgaaaat caataaagcc aaaattatag aattcctatc aaaattgaac gcaattgaaa
     6541 agcaacatac ccaaaagcaa catcccgaca aacaaaatac tcacaaacaa taatcaaagc
     6601 tttaaaatta acgatacatc cctaaacgtt agttttaaag cttttttatt atccttatca
     6661 acttatcaac taataagctt atataaccac ttgctcataa aagcatcacc tttaaagtga
     6721 aatcactata gattttaaaa caaatttttc aaaagaaaca agaaaattta atacaaaaac
     6781 taatttatta attaaagaat aaacatataa aatataagct atacaataac accatattta
     6841 aacccaacac tatatataga atggaaggcg aaaacaagat aatgacctgc ataagaaatt
     6901 taaaatctac atatttaaac taaattagca taaaatgaca aaggctacct accgtagcca
     6961 acctattatt ctaatcaaac aataagagtg agacttcaaa tctcagtcta taggataatt
     7021 ataaactatt ttattttttg taaagtcttt tttgaaagtt tttatgctat ttaaaaaata
     7081 ttctcaagaa cctattatca aaacttgata agttatcaaa ccaatataaa tacccaatta
     7141 agacacaatg gggttattgt acttttacag tattatatct atcgcgcatc ttattaaaaa
     7201 aatctttttc attcttgtat atttctttaa gcaaaaagct agcaaatttt gtattggatt
     7261 tgtaaaaatg aaaactctct tcatcatcta gttgaaacct taaaggtctt agagggttga
     7321 cattgctttt actaatgata ttagtatttt ccagcacctt aattgcataa tctacccctc
     7381 tacttaaaat caaatcataa tcaatcttac cgctatcaat tcctgtaact aacttaatat
     7441 acctatatat ggaagttttt gctattttat agctcttgat aaaagctgta aaacttttat
     7501 atccatcaag agcatagtat ttgccctagg
//
