LOCUS       62056_18270        3677 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     423..439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(716..732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(740..756)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(764..794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(809..830)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1118..1706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1910..2710
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     oriT            3155..3264
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcgcag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
      121 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttg atcggcactt
      181 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
      241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
      301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      421 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagta tgcatgcgag
      481 tgtccgttcg agtggcggct tgcgcccgat gctagtcgcg gttgatcggc gatcgcaggt
      541 gcacgcggtc gatcttgacg gctggcgaga ggtgcgggga ggatctgacc gacgcggtcc
      601 acacgtggca ccgcgatgct gttgtgggca caatcgtgcc ggttggtagg atccacatat
      661 gttggggatc ctctagagga tccgcggccg cgcgcgatat cgaattcgta atcatgtcat
      721 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      781 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      841 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      901 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      961 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1801 tcacctagat ccttttggtt catgtgcagc tccatcagca aaaggggatg ataagtttat
     1861 caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc
     1921 ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct
     1981 caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg
     2041 gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctgcgc tgggtccggg
     2101 agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac
     2161 gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt
     2221 aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat
     2281 ctctgatcca ttgcccctgc cacctcactc gcctgcaagc ccggtcgccc gtgtccatga
     2341 actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct
     2401 tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga
     2461 tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt
     2521 ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt
     2581 tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga
     2641 tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca
     2701 gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct
     2761 gattgctggg gcaacacgtg gagcggatcg gggattgtct ttcttcagct cgctgatgat
     2821 atgctgacgc tcaatgccgt ttggcctccg actaacgaaa atcccgcatt tggacggctg
     2881 atccgattgg cacggcggac ggcgaatggc ggagcagacg ctcgtccggg ggcaatgaga
     2941 tatgaaaaag cctgaactca ccgcgacgta tcgatggtac catatattta aagatccact
     3001 agtatgcagg tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga
     3061 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     3121 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     3181 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     3241 atcctgctct gcgaggctgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca
     3301 gggctacaaa atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa
     3361 tggcgacctg ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac
     3421 ggcgcggttc ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga
     3481 cgagcttggc aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta
     3541 gccgctaaaa cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca
     3601 agaagagcga cttcgcggag ctggtgaagt acatcaccga cgagcaaggc aagaccgatc
     3661 cccggggacc tgcatag
//
