LOCUS       40924_33717       10257 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pOEHyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Chen L.
  TITLE     Expression system for Pestalotiopsis microspora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Chen L.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2016) State Key Program of Microbiology and 
            Department of Microbiology, College of Life Sciences, Nankai 
            University, No. 94 Weijin Road, Tianjin 300071, China
REFERENCE   3  (bases 1 to 10257)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-2016) State Key Program of Microbiology and Department of 
            Microbiology, College of Life Sciences, Nankai University, No. 94 
            Weijin Road, Tianjin 300071, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1742..1766)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        1850..1868
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3396..3750
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             3755..4777
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    complement(5464..5488)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6016..6804
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7051..7639
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7825..7965)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8309..8503)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8572..9636)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(join(10073..10257,1..442))
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWASADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRNGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
       61 cctcaatgcg ctgcttaacc agctccacca ggtcggcgga ggcccatatg tcgtaagggc
      121 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
      181 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
      241 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
      301 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
      361 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
      421 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
      481 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
      541 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
      601 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
      661 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
      721 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
      781 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
      841 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
      901 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
      961 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
     1021 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
     1081 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
     1141 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
     1201 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgcctgggcg taggtggtca
     1261 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
     1321 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
     1381 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
     1441 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
     1501 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
     1561 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
     1621 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
     1681 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta
     1741 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
     1801 caatctgatc caagctcaag ctaagcttca ggaaacagct atgaccatgt aatacgactc
     1861 actatagggg atatcggcgc gcctctagaa tttaaatgga tccgtgtagg tgggtaaatg
     1921 ccaatcattt ggtggtatat aatatacatt ttgcatgtgc gactgccgtt ccgttttttt
     1981 tgaattacct aggttgtttt cgacttgccc ccgaaaagcc aggaaactta gcttttcgtg
     2041 tctttttttt gttcaaattt acttctttgc tacactgaat tgttggaccc ttcctttgcg
     2101 ggagccttcg cactatacac actatacgat agagaaggtt ctctcctatt ctcaaaaggc
     2161 aacccactca ctcttcaggg actacatttg cttagcttgc aggctccggc ggcccaatac
     2221 gtacgagctg ctttgcttct tcttgatccc ttttgaccca tttcctcttg gatacttacg
     2281 tattggagtt ggggaaaagg gaccgtttca ttgccaacct aaccaagctt ccggctacgg
     2341 taatagggga aaggaaccaa cctacctact acctaggatg aaagcgaaca gtttggagag
     2401 ctaagccggc aaacaaacgc atttggcaac gaagaacgaa tgttccacgg tcttggcttc
     2461 ggtgcatcgt ttaataaggc agacaggata gatagactag atggagcacg cacaggcaga
     2521 gaaggagaag agaaggagag gggagaggtt ctttttaggg ggtgtccagg cttgggtcgc
     2581 tcctttgtgc aattccggga gtgaacccac aactgtgatt cgatgattag gtgggcgagg
     2641 aagagaaagg gaaaggcggg cgcccactga ctgacgggga cagcccactt ggtcgcccat
     2701 ggccctgcac cgcacccgat tgcactgcac tggacgcacg cactggcggg cactgggtac
     2761 tgggccggca ctccctggac tgggtggact gggacctact gactggagct gggcctttcc
     2821 ctttttccca cctcttccct cctccaccgc atcaaccatc actccgacca ttgacaacac
     2881 attttaaggt ccatctccca gcctcctttc cttgtccgct actccattgc agacatcgag
     2941 actcttttcc atccccaaga ccatcacatc acgccaaaag ctcccatacc gtgagcttca
     3001 ttcttcttcc tacattcact tctactcttc gtcaccttac tactactaat ccgccaaagt
     3061 cgacctaact gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct
     3121 tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact
     3181 gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca
     3241 aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga
     3301 tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt
     3361 taatatgagg agtaaaacat cccaaacaag cccaactgat attgaaggag cattttttgg
     3421 gcttggctgg agctagtgga ggtcaacaat gaatgcctat tttggtttag tcgtccaggc
     3481 ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat ttaggcaact ggtcagatca
     3541 gccccacttg tagcagtagc ggcggcgctc gaagtgtgac tcttattagc agacaggaac
     3601 gaggacatta ttatcatctg ctgcttggtg cacgataact tggtgcgttt gtcaagcaag
     3661 gtaagtggac gacccggtca taccttctta agttcgccct tcctcccttt atttcagatt
     3721 caatctgact tacctattct acccaagcat ccaaatgaaa aagcctgaac tcaccgcgac
     3781 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     3841 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     3901 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     3961 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
     4021 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     4081 cgctgttctc cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     4141 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     4201 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     4261 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     4321 cgaagtccgg cacctcgtgc atgcggattt cggctccaac aatgtcctga cggacaatgg
     4381 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     4441 cgccaacatc ctcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     4501 cgagcggagg catccggagc ttgcaggatc gccgcgcctc cgggcgtata tgctccgcat
     4561 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     4621 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     4681 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     4741 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgggaac
     4801 cagttggcag gttgagtgag tgaggagctc tccaaaagct ctcctcacct tgttctaagt
     4861 tctcaagtcg tttctcaccc atctgcttac aaacattgct ttgagaagga gaatcgagcc
     4921 ctgtgaaccc gatccgcccc gtcggccgcc agcagatttg gttatacacg accaaagaca
     4981 caccgagagg aagggaaaag gaagagcgga aagacgccga acgattcccg ggactggatg
     5041 ggacctgcgc aagccaacgg cgacgatcgc gccggcgtcg ataccaagat ggaggagacc
     5101 accccgccga acgacacatc gcaaccgatc aagcccccgc cgcggaaacg ccgccgagtc
     5161 gtcatctcgt gtctggactg ccacaagcgc aagcagaagt gcgacagaaa actgccctgc
     5221 acaaattgcg tctcgagagg gaaacagtcg tcatgtcgct acgagacggg cgcgcccatc
     5281 tctaaggacg ggcacgaggg gcgcaaaggc tcacagccgg gagaagacgc atcgccgcct
     5341 ctggatgctg ctggtagcaa cggcgaagga ggccacatca acatcaaggg aattcaattc
     5401 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     5461 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     5521 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     5581 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     5641 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     5701 tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctcgt
     5761 tcggttcgta agctgtaatg caagtagcgt aactgccgtc acgcaactgg tccagaacct
     5821 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttct tgttatgaca
     5881 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     5941 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6001 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     6061 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6121 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6181 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6241 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6301 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6361 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     6421 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6481 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6541 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6601 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     6661 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     6721 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     6781 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     6841 cttcgccggc gttaactcaa gcgattagat gcactaagca cataattgct cacagccaaa
     6901 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     6961 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7021 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7081 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7141 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7201 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7261 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7621 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7681 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7741 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7801 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     7861 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7921 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     7981 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8041 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8101 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     8161 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     8221 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     8281 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     8341 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     8401 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     8461 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     8521 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     8581 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     8641 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
     8701 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
     8761 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
     8821 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
     8881 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
     8941 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
     9001 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
     9061 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
     9121 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
     9181 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
     9241 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
     9301 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
     9361 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
     9421 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
     9481 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
     9541 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
     9601 cgcgccgatt tgtaccgggc cggatggttt gcgaccgtca cgccgattcc tcgggcttgg
     9661 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     9721 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     9781 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     9841 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     9901 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     9961 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
    10021 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
    10081 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
    10141 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
    10201 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgt
//
