LOCUS       40924_33707        8429 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"prey"""" vector for fusing a gene to the GAL4 
            activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, 
            Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li 
            Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, 
            Johnston M, Fields S, Rothberg JM.
  TITLE     A comprehensive analysis of protein-protein interactions in 
            Saccharomyces cerevisiae.
  JOURNAL   Nature 2000;403:623-7.
  PUBMED    10688190
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Fields Lab
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2000"; volume: "403"; pages: "623-7"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      408..595
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(1427..2589)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     CDS             complement(2954..4045)
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(4058..4462)
                     /label=LEU2 promoter
     promoter        4568..4672
                     /label=AmpR promoter
     CDS             4673..5530
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5704..6292
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        7281..7981
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             8026..8046
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             8062..8403
                     /codon_start=1
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 aattcccggg gatccgtcga cctgcagaga tctatgaatc gtagatactg aaaaaccccg
       61 caagttcact tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt
      121 tgccttcttt tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat
      181 ctatagaatt ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt
      241 catgaaaata tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg
      301 tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg
      361 aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat
      421 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
      481 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
      541 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
      601 cgagcaaatg cctgcaaatc gctccccatt tcacccaatt gtagatatgc taactccagc
      661 aatgagttga tgaatctcgg tgtgtatttt atgtcctcag aggacaacac ctgttgtaat
      721 cgttcttcca cacggatcct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      781 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
      841 gtgcggtatt tcacaccgca tatatcgctg ggccattctc atgaagaata tcttgaattt
      901 attgtcatat tactagttgg tgtggaagtc catatatcgg tgatcaatat agtggttgac
      961 atgctggcta gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat
     1021 caaatatcaa acttaactat tgactttata acttatttag gtggtaacat tcttataaaa
     1081 aagaaaaaaa ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc
     1141 tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa
     1201 tacatataat acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct
     1261 aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt
     1321 tgtaaaagtt cactttagca tacatatatt acacgagcca gaaattgtaa cttttgccta
     1381 aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt
     1441 ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt
     1501 attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg
     1561 gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt
     1621 acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg
     1681 tcacagtttt acagtaaata agtatcacct cttagagttc gatgataagc tgtcaaacat
     1741 gagaattaat tccacatgtt aaaatagtga aggagcatgt tcggcacaca gtggaccgaa
     1801 cgtggggtaa gtgcactagg gtccggttaa acggatctcg cattgatgag gcaacgctaa
     1861 ttatcaacat atagattgtt atctatctgc atgaacacga aatctttact tgacgacttg
     1921 aggctgatgg tgtttatgca aagaaaccac tgtgtttaat atgtgtcact gtttgatatt
     1981 actgtcagcg tagaagataa tagtaaaagc ggttaataag tgtatttgag ataagtgtga
     2041 taaagttttt acagcgaaaa gacgataaat acaagaaaat gattacgagg atacggagag
     2101 aggtatgtac atgtgtattt atatactaag ctgccggcgg ttgtttgcaa gaccgagaaa
     2161 aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt gaacattctc ttcaacaagt
     2221 ttgattccat tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat tttcctgtac
     2281 aatcaatcaa aaagccaaat gatttagcat tatctttaca tcttgttatt ttacagattt
     2341 tatgtttaga tcttttatgc ttgcttttca aaaggcttgc aggcaagtgc acaaacaata
     2401 cttaaataaa tactactcag taataaccta tttcttagca tttttgacga aatttgctat
     2461 tttgttagag tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac
     2521 gccatttaat ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa
     2581 atgtaagctc tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa
     2641 aagttcacct gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg
     2701 tgaagctgca ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg
     2761 caaaccgagg aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatcga
     2821 tgataagctg tcaaacatga gaattaattc taccctatga acatattcca ttttgtaatt
     2881 tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat cataaaaaaa
     2941 gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca ccgacttcgg
     3001 tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa acctttttaa
     3061 ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc aacatcattg
     3121 cagcagacaa gatagtggcg atagggtcaa ccttattctt tggcaaatct ggagcagaac
     3181 cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg
     3241 cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag atgatatcac
     3301 caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta actaggatca
     3361 tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg
     3421 tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct ttatccaagg
     3481 accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt cttgtgattc
     3541 tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca ccatcgtctt
     3601 cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg aagtcagtac
     3661 ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat gcaaagttac
     3721 atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa ccttgttcag
     3781 gtctaacact acctgtaccc catttaggac cacccacagc acctaacaaa acggcatcaa
     3841 ccttcttgga ggcttccagc gcctcatctg gaagtgggac acctgtagca tcgatagcag
     3901 caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca tcagaaatag
     3961 ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca cctggcaaaa
     4021 cgacgatctt cttaggggca gacatagggg cagacattag aatggtatat ccttgaaata
     4081 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     4141 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     4201 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcctctca
     4261 cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga
     4321 aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt
     4381 ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg
     4441 atacctgagt attcccacag ttaattcttg aagacgaaag ggcctcgtga tacgcctatt
     4501 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4561 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4621 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4861 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4981 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5221 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5281 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5521 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5821 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     5881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6241 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6361 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6421 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga tccgggatcg
     6541 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa
     6601 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg
     6661 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg
     6721 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat
     6781 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg
     6841 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct
     6901 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt
     6961 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct
     7021 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat
     7081 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt
     7141 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag
     7201 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta
     7261 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc
     7321 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
     7381 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
     7441 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
     7501 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     7561 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     7621 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga
     7681 aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta
     7741 tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca
     7801 acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt
     7861 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     7921 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     7981 ccaagctttg caaagatgga taaagcggaa ttaattcccg agcctccaaa aaagaagaga
     8041 aaggtcgaat tgggtaccgc cgccaatttt aatcaaagtg ggaatattgc tgatagctca
     8101 ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac tcaaacaaat
     8161 tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt catgaataat
     8221 gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc acctggttgg
     8281 acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa taccactaca
     8341 atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa cccaaaaaaa
     8401 gagatcgaat tccagctgac caccatggc
//
