LOCUS       62056_18030        4571 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    60..151
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     CDS             636..2153
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="VLGPCMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAA
                     KKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKT
                     YNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGK
                     SVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVIL
                     GGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLD
                     PSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHG
                     HHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGL
                     APGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGED
                     VAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    complement(2216..2337)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(2437..2831)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2848..3219)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3239..3286)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     LTR             complement(3316..3584)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3597..3808)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     rep_origin      complement(3876..4464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgtaacta
       61 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
      121 agtgcaagtg caggtgccag aacatttctc tatcgaagga tctgcgatcg ctgaattagt
      181 ttcactttcc agtttcagtt tccagtttca ttttccagtt tcattttcca gtttcatttt
      241 cctgatatcc tgcaggagct tgaataaaat gaatattaga agctgttaga ataagagaaa
      301 atgacagagg aaaactgaaa gggagaactg aaagtgggaa attcctctga ggcagaaagg
      361 accatccctt ataaatagca caggccatga aggaagatca ttctcactgc agcctttgac
      421 agcctttgcc tcatcttgaa gcttctgcct tctccctcct gtgagtttgg taagtcactg
      481 actgtctatg cctgggaaag ggtgggcagg agatggggca gtgcaggaaa agtggcacta
      541 tgaaccctgc agccctagga atgcatctag acaattgtac taaccttctt ctctttcctc
      601 tcctgacagg ttggtgtaca gtagcttcca ccatggttct ggggccctgc atgctgctgc
      661 tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca gttgaggagg
      721 agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc
      781 agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg atgggggtgt
      841 ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg gggcctgaga
      901 tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac aatgtagaca
      961 aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc aagggcaact
     1021 tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg acacgcggca
     1081 acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa
     1141 ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg gtgaaccgca
     1201 actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc caggacatcg
     1261 ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc cgaaagtaca
     1321 tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa ggtgggacca
     1381 ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg
     1441 tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc catctcatgg
     1501 gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca ctggacccct
     1561 ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc cgcggcttct
     1621 tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg gcttaccggg
     1681 cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag ctcaccagcg
     1741 aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc ttcggaggct
     1801 accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg gacaggaagg
     1861 cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac ggcgcccggc
     1921 cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc
     1981 tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc
     2041 acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc ttcgccgcct
     2101 gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc
     2161 acccggggcg gtcccggtcc aagcgtctgg attgaagcta gctggccaga catgataaga
     2221 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2281 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2341 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2401 agcaagtaaa acctctacaa atgtggtatg gaattctaaa atacagcata gcaaaacttt
     2461 aacctccaaa tcaagcctct acttgaatcc ttttctgagg gatgaataag gcataggcat
     2521 caggggctgt tgccaatgtg cattagctgt ttgcagcctc accttctttc atggagttta
     2581 agatatagtg tattttccca aggtttgaac tagctcttca tttctttatg ttttaaatgc
     2641 actgacctcc cacattccct ttttagtaaa atattcagaa ataatttaaa tacatcattg
     2701 caatgaaaat aaatgttttt tattaggcag aatccagatg ctcaaggccc ttcataatat
     2761 cccccagttt agtagttgga cttagggaac aaaggaacct ttaatagaaa ttggacagca
     2821 agaaagcgag cttctagctt atcctcagtc ctgctcctct gccacaaagt gcacgcagtt
     2881 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2941 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     3001 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     3061 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     3121 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     3181 cgcggtgagc accggaacgg cactggtcaa cttggccatg atggccctcc tatagtgagt
     3241 cgtattatac tatgccgata tactatgccg atgattaatt gtcaactact gtttgtaggc
     3301 gccggtcaca gcttggatct gtaacggcgc agaacagaaa acgaaacaaa gacgtagagt
     3361 tgagcaagca gggtcaggca aagcgtggag agccggctga gtctaggtag gctccaaggg
     3421 agcgccggac aaaggcccgg tctcgacctg agctttaaac ttacctagac ggcggacgca
     3481 gttcaggagg caccacaggc gggaggcggc agaacgcgac tcaaccggcg tggatggcgg
     3541 cctcaggtag ggcggcgggc gcgtgaagga gagatgcgag cccctcgaag cttcagctgt
     3601 gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac
     3661 ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt
     3721 accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc caacttctcg
     3781 gggactgtgg gcgatgtgcg ctctgcccac tgacacatgt gagcaaaagg ccagcaaaag
     3841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3901 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3961 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4021 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4081 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4141 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4201 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4261 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4321 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4381 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4441 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4501 cagtggaacg aaaactcacg ttaagggatt ttggtcatgg ctagttaatt aacatttaaa
     4561 tcagcggccg c
//
