LOCUS       62056_18025        4406 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    16..64
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    75..166
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     CDS             460..1977
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="VLGPCMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAA
                     KKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKT
                     YNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGK
                     SVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVIL
                     GGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLD
                     PSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHG
                     HHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGL
                     APGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGED
                     VAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    complement(2040..2161)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(2261..2655)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(2672..3043)
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3063..3110)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     LTR             complement(3140..3408)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3421..3632)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     rep_origin      complement(3726..4314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taaatcagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
       61 tgtgaatcgt aactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
      121 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg aaggatctgc
      181 gatcgctgaa ttctggggac tttccactgg ggactttcca ctggggactt tccactgggg
      241 actttccact ggggactttc cactcctgca gcagtggata ttcccagaaa actttttgga
      301 tgcagttggg gatttcctct ttactggatg tggacaatat cctcctatta ttcacaggaa
      361 gcaatccctc ctataaaagg gcctcagcag aagtagtgtt cagctgttct tggctgactt
      421 cacatcaaag cttctatact gacctgagac agagccatgg ttctggggcc ctgcatgctg
      481 ctgctgctgc tgctgctggg cctgaggcta cagctctccc tgggcatcat cccagttgag
      541 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
      601 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
      661 gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa actggggcct
      721 gagatacccc tggctatgga ccgcttccca tatgtggctc tgtccaagac atacaatgta
      781 gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg ggtcaagggc
      841 aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa cacgacacgc
      901 ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc agtgggagtg
      961 gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca cacggtgaac
     1021 cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg gtgccaggac
     1081 atcgctacgc agctcatctc caacatggac attgatgtga tcctgggtgg aggccgaaag
     1141 tacatgtttc gcatgggaac cccagaccct gagtacccag atgactacag ccaaggtggg
     1201 accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg
     1261 tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt gacccatctc
     1321 atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc cacactggac
     1381 ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc
     1441 ttcttcctct tcgtggaggg tggtcgcatc gaccacggtc atcacgaaag cagggcttac
     1501 cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg ccagctcacc
     1561 agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt ctccttcgga
     1621 ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc ccgggacagg
     1681 aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa ggacggcgcc
     1741 cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca gtcagcagtg
     1801 cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag
     1861 gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat ggccttcgcc
     1921 gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc
     1981 gcgcacccgg ggcggtcccg gtccaagcgt ctggattgaa gctagctggc cagacatgat
     2041 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2101 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2161 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2221 ttaaagcaag taaaacctct acaaatgtgg tatggaattc taaaatacag catagcaaaa
     2281 ctttaacctc caaatcaagc ctctacttga atccttttct gagggatgaa taaggcatag
     2341 gcatcagggg ctgttgccaa tgtgcattag ctgtttgcag cctcaccttc tttcatggag
     2401 tttaagatat agtgtatttt cccaaggttt gaactagctc ttcatttctt tatgttttaa
     2461 atgcactgac ctcccacatt ccctttttag taaaatattc agaaataatt taaatacatc
     2521 attgcaatga aaataaatgt tttttattag gcagaatcca gatgctcaag gcccttcata
     2581 atatccccca gtttagtagt tggacttagg gaacaaagga acctttaata gaaattggac
     2641 agcaagaaag cgagcttcta gcttatcctc agtcctgctc ctctgccaca aagtgcacgc
     2701 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
     2761 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
     2821 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
     2881 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
     2941 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     3001 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catgatggcc ctcctatagt
     3061 gagtcgtatt atactatgcc gatatactat gccgatgatt aattgtcaac tactgtttgt
     3121 aggcgccggt cacagcttgg atctgtaacg gcgcagaaca gaaaacgaaa caaagacgta
     3181 gagttgagca agcagggtca ggcaaagcgt ggagagccgg ctgagtctag gtaggctcca
     3241 agggagcgcc ggacaaaggc ccggtctcga cctgagcttt aaacttacct agacggcgga
     3301 cgcagttcag gaggcaccac aggcgggagg cggcagaacg cgactcaacc ggcgtggatg
     3361 gcggcctcag gtagggcggc gggcgcgtga aggagagatg cgagcccctc gaagcttcag
     3421 ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat
     3481 atacggttct cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca
     3541 gtttaccccg cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt
     3601 ctcggggact gtgggcgatg tgcgctctgc ccactgacta gtgggccctg caggttaatt
     3661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4381 ttggtcatgg ctagttaatt aacatt
//
