LOCUS       Exported                3167 bp ds-DNA     circular SYN 17-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pNZ8148
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   caoheibi
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        5..188
                     /note="Pnis"
                     /note="Nisin A promoter region"
     terminator      393..445
                     /note="T"
     CDS             788..997
                     /codon_start=1
                     /note="repC"
                     /note="Replication gene C"
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIFVFMITK"
     CDS             1266..1964
                     /codon_start=1
                     /note="repA"
                     /note="Replication gene A"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             2424..3074
                     /codon_start=1
                     /note="cm"
                     /note="chloramphenicol resistant gene"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
ORIGIN
        1 agatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa
       61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg
      121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa
      181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga
      241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa
      301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg
      361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
      421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat
      481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg
      541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg
      601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt
      661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa
      721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg
      781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg
      841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg
      901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt
      961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt
     1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga
     1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg
     1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag
     1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt
     1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact
     1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc
     1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac
     1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg
     1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc
     1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg
     1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt
     1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac
     1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc
     1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa
     1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa
     1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga
     1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga
     2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat
     2101 tattgaataa ataaaagccc ccctgacgaa agtcgacggc aatagttacc cttattatca
     2161 agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac acaaaagacc
     2221 acatttttta atgtggtctt ttattcttca actaaagcac ccattagttc aacaaacgaa
     2281 aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat attgactttt
     2341 aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat tcactttaga
     2401 taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac aattggaaga
     2461 gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata accacagaaa
     2521 ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt taccctgcat
     2581 ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact ggttacaata
     2641 gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt tttgatggtg
     2701 tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa gagttttatg
     2761 atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt cccaaaacac
     2821 ctatacctga aaatgctttt tctctttcta ttattccttg gacttcattt actgggttta
     2881 acttaaatat caataataat agtaattacc ttctacccat tattacagca ggaaaattca
     2941 ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat tctgtttgtg
     3001 atggttatca tgctggattg tttatgaact ctattcagga attgtcagat aggcctaatg
     3061 actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt tttctaatgt
     3121 cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctcc
//
