LOCUS       40924_33697        9809 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNZ4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
  TITLE     A method for mating clinical Candida albicans isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   Zhang N, Magee BB, Magee PT, Cannon R, Schmid J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2009) IMBS, Massey University, Riddet Road, 
            Palmerston North 4410, New Zealand
REFERENCE   3  (bases 1 to 9809)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAR-2009) IMBS, Massey University, Riddet Road, Palmerston North
            4410, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     mobile_element  683..1491
                     /mobile_element_type="insertion sequence:TS1"
                     /label=insertion sequence:TS1
                     /note="from Candida albicans chromosome 7"
     terminator      1644..1831
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        1852..1907
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     misc_feature    2083..4189
                     /label=IMH3r ORF plus terminator from plasmid p3408
                     /note="IMH3r ORF plus terminator from plasmid p3408"
     CDS             join(2083..2533,2781..3895)
                     /codon_start=1
                     /product="IMH3r"
                     /label=IMH3r
                     /protein_id="ACY78684.1"
                     /translation="MVFETSKATSYLKDYPKKDGLSVKELIDSTNFGGLTYNDFLILPG
                     LVNFPSSAVSLETKLTKKITLKSPFVSSPMDTVTEENMAIHMALLGGIGIIHHNCTAEE
                     QAEMVRKVKKYENGFINDPVVISPEVTVGEVKKMGEVLGFTSFPVTENGKVGGKLVGII
                     TSRDIQFHEDNKSPVSEVMTKDLVVGKKGISLTDGNELLRSSKKGKLPIVDAEGNLVSL
                     ISRTDLQKNQDYPNASKSFHSKQLLCGAAIGTIDADRERLDKLVEAGLDVVVLDSSNGS
                     SVFQLNMIKWIKEKYPELQVIAGNVVTREQAALLIEAGADALRIGMGSGSICITQEVMA
                     CGRPQGTAVYGVTEFANKFGVPCIADGGIGNIGHITKALALGASCVMMGGLLAGTAETP
                     DDYFYRDGKRLKTYRGMGSIDAMQQTNTNANASTSRYFSEADKVLVAQGVSGSVVDKGS
                     ITKFVPYLYNGLQHSLQDIGIKSIDELRENVDNGEIRFEFRTASAQFEGGVHGLHSYEK
                     RLHN"
     CDS             complement(4462..4542)
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     CDS             complement(5239..5859)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      5860..6387
                     /label=PENO1 from plasmid pCAITHE5
                     /note="PENO1 from plasmid pCAITHE5"
                     /regulatory_class="promoter"
     mobile_element  6404..7560
                     /mobile_element_type="insertion sequence:TS2"
                     /label=insertion sequence:TS2
                     /note="from Candida albicans chromosome 7"
     primer_bind     complement(7575..7591)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7621..7639)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7660..7676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7684..7700)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7708..7738)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7753..7774)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8062..8650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8824..9681)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9682..9786)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgccccg ggtcctttct tactaaaata tagatataga tataattata
      721 atcactgaat tgatctagtt attgttaagt gatttcaatt gtggttttgt aatgtaaaaa
      781 gtagagacat aagtaaacat atcagcatta ttatgtatac ccaattccat attccaacta
      841 gaaaacatat aaagtttata cctcagaagg atacaaaaaa aaaaaaaaaa gataatacga
      901 atgtttctga ttctagtagt aatgtgtttt agttttagtg ataaactttt ttttttctta
      961 attaactgca aagctcaaat taataaatat atttcttata tatgaattag ttaaagtctt
     1021 actattcttt agatattttg ttcaaaaaca ataaaacaca atctagattc atagtagttg
     1081 agtttaatag attgaataag gcgtttcttg aaaataaaga taaaaccaaa tgaaattatg
     1141 ttacaatgaa tcccctcccc aaaaaaaaaa gtcaatcaat actatataca aaaggtaata
     1201 taacactatt atgatgggtc tagaaaaggg tcttggaaag tggggctaaa gtttgtaatg
     1261 ataagttgca actagaatca gcccatccct ctctctcctt ttgttacgag cttattttca
     1321 aatcaaggtt ttagtttctt tgtttgaaat tagtaactta ttttaatagc aagaacagct
     1381 aaaagtttta ccatttcttt ctttcttttt aactaattat caatctcata tttagttgtt
     1441 ttgatttatt gttttgcaaa taacaattta actgattgtg tgtgtaagat cgcggccgct
     1501 ctagaactag ggtggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg
     1561 gcttgtctac cttgccagaa atttacgaaa agatggaaaa gggtcaaatc gttggtagat
     1621 acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat
     1681 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     1741 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
     1801 aggtcgctct tattgaccac acctctaccg gcatgcgagc tcctaatttt tgttgacact
     1861 ctatcattga tagagttatt ttaccactcc ctatcagtga tagagaaaag tggatccaat
     1921 tgtactttat atatagttga aacaacaggg aaagggcagg ataaagaaag aaattgactg
     1981 cagtttccta tgatcatatt tcatttttgt aattaacaat ataatataat acacttataa
     2041 acggtttaaa aacagcttta tctcagaaaa gtcgacctgc agatggtgtt tgaaacttca
     2101 aaagctactt cttatttgaa agattaccct aagaaagatg gtttatcggt caaagaattg
     2161 attgattcta ctaattttgg tgggttaact tataatgatt tcttaatttt accaggttta
     2221 gtcaatttcc ctagttcagc tgtttcatta gaaactaaat tgactaaaaa aatcactttg
     2281 aaatcaccat ttgtttcatc acctatggat actgttactg aagaaaatat ggctattcat
     2341 atggcattat tgggtggtat tggtatcatt catcataact gtactgctga agaacaagca
     2401 gaaatggtta gaaaagtcaa aaaatacgaa aatggtttca ttaatgaccc agttgtcatt
     2461 tctcctgaag tcactgttgg tgaagttaaa aaaatgggtg aagttttagg tttcacttct
     2521 ttcccagtca ctggtatgtt tctttaattt gtgtttgttt gttttccgaa aaaaggtatc
     2581 acattatact attactctga tgattcttta aaatttatta actttcgttc taccgatacc
     2641 atcaaacgat gttggggaaa aaactttatt aatctgattc ttttaaaaat tcaacttttc
     2701 aacaatgtga tatcttagaa aacaaacaca tcaaccaaga aaattaacat ttttttacta
     2761 acaattattg tataatatag aaaatggtaa agttggcggt aaattagttg gtattatcac
     2821 ttctcgtgat attcaattcc atgaagataa caaatcacca gtttctgaag ttatgaccaa
     2881 agatttagtt gttggtaaga aaggaatttc tttaaccgat ggtaatgaat tgttaagatc
     2941 ttctaaaaaa ggtaaattac caattgttga tgctgaaggt aatttggtgt ctttgatttc
     3001 tcgtaccgat ttacaaaaga atcaagatta tccaaatgct tcgaaatcat tccattctaa
     3061 acaattatta tgtggtgctg ccattggtac cattgatgct gatagagaaa gattggacaa
     3121 attagtagaa gctggattag atgttgttgt attagattca tctaatggtt catcagtttt
     3181 ccaattgaac atgatcaaat ggatcaaaga aaaataccca gaattacaag ttattgctgg
     3241 taatgttgtc actagagaac aagcagcatt attgattgaa gctggtgccg atgccttgag
     3301 aattggtatg ggttctggtt ctatttgtat tactcaagaa gttatggctt gtggtagacc
     3361 tcaaggtact gctgtttatg gtgtcactga atttgccaac aaatttggtg ttccatgtat
     3421 tgctgatggt ggtattggta acattggtca catcaccaag gctttagctt taggtgcttc
     3481 ttgtgttatg atgggtggtt tattggctgg tactgccgaa accccagatg attacttcta
     3541 cagagatggt aaaagattga agacttatag aggtatgggt tccatcgatg ccatgcaaca
     3601 aaccaacacc aatgctaatg cttctacttc aagatatttc tcagaagctg ataaagtatt
     3661 ggttgcccaa ggtgtctctg gttcagtcgt tgataaaggt tccatcacca agtttgtacc
     3721 atacttgtac aatgggttac aacattcttt acaagatatt ggtattaaat caattgacga
     3781 attgagagaa aacgttgata atggtgaaat tagatttgaa tttagaactg cttcagctca
     3841 attcgaaggg ggcgttcatg gtttacactc ttatgaaaag agattacaca actaagtttt
     3901 ttgcatgtat taagtattct taattggaag tagaaagagt tttttgttgt ataggtaata
     3961 tttttgtatt tttttgtttt taatttgatt tgatatgatt tttagacgca cgtgacacag
     4021 acacacagcc cagtgaagag tttagctcct tcgttttttc ttcgttgatt tcttgatcca
     4081 cacattaaca catttacatc ccttatctaa actacaataa tgcccattcc tcctacatct
     4141 acagaaaata aaaacttaga tgaagatata ctgagttcta gactcgagat cgaattcctg
     4201 cagccccccc catggtgaga cgcgacagac agacaccaca cacttggtag caatcatcgg
     4261 tattccttgt atttgcccca cacaaatcta caaatttgac attaattaat agcttcacat
     4321 ttattaaaga atataaaata aactaaagaa tattaaaaaa aaacgttata aaaatagata
     4381 gctcaatggc tcatctaagc gaaacaaaat catagcaaga cttcaactca attgaggtta
     4441 ctcgggccct caatcacagc tagcgtaatc cggtacatcg tatgggtaag cgtaatccgg
     4501 cacatcgtaa gggtaagcgt aatccggaac atcgtatggg tagctagctg ctcgagaaaa
     4561 tacttgtacc tttaaaaaat cgacatcttc gtcaaggtcg tcgatgaaac tgctttgatc
     4621 aagttcattg gtggcagttg catcattgtt gttatcgttt aagtcataaa ggttagcgtt
     4681 cgacttcgac gtagaaattt ttttggattt aagtactttg gtgtagtcgt catcagtacc
     4741 ggcactaccg tcaataccgg tatcgttgga aaacatgaaa tttttaaaag atctgccatt
     4801 gttacagtcc tgctcttcca tcaactcttc tagagttggg ggcaaataag aaaacttatt
     4861 gtcagccaaa gtgatttctg aacctaactc gtcatggtga tgcgaatcag cgctttcatc
     4921 ctgatcctga agaaacaaat aggtttcatc gttctttgct tggatgttcg ccttctcaga
     4981 attagtattt tttttcaaag cacgtgatga atcttcttgt tgtgaacttg aacttggtaa
     5041 ataattccaa atgtcttctt catctggctc aaccttatca tcttgccctg aagatacggt
     5101 ttcctccaaa attggcaaat tcaataaaga catgttatcg tcattgtcca cttgttggta
     5161 attgccactg ttcttacctg ttgtatcgtc catagactgt aagtaagaat tccagagatc
     5221 attattttcg ttaccgcggg acccactttc acatttaagt tgtttttcta atccgcatat
     5281 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     5341 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     5401 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     5461 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     5521 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     5581 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     5641 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     5701 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     5761 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gaagctctaa
     5821 tgcgctgtta atcactttac ttttatctaa tctagacatc tgcagaatat tcctgaatta
     5881 tcaattgatg attgaatgaa gaattaaaaa aaagggagaa aaggaaagaa atagaaacag
     5941 aaaaaaaaag aaaaaacagg aattggaaaa tcagacttat tttatattaa ttggagtcta
     6001 aaagatctta ctgtgagcaa aattgtcaat caacaataaa gggagtaagg tggttgaaaa
     6061 attcagaaat gaaataacct gacggcgcgc atacacaaaa acttactatg gtggtggtgg
     6121 gtgggctact catatgcagt agtagacata taatgaatgg aacacaactt cccgatgcac
     6181 tttttttttt ttttctgttg ccctagtgtg gttatgttct atgcaatgaa gagagaaatc
     6241 tggccaacat caggtgaacg caggaaaagt atgtctgttt gtacgtttgt ataattagtg
     6301 tggatcacct tatagaaatc gttagtcaac ttttgcaacg cataagttct aaaaaaaggc
     6361 atcacctgta aacaaatctt gatcccatcg atgaatcaag cttgaaagaa ggaaagaagg
     6421 aaagaaggaa gacagttgtc cacattgtag agtcattttc attgtcattt tcaccaccag
     6481 ggtcaatatt gagcatttat aatcctctct ttcttcgtca aagtagtatc tttttctctc
     6541 gcttcgctta tgtcatcttg attcaataaa ttaatccgta attgacatta acgtaataat
     6601 agccgtagct gtattatcac ataatgtaag taaaaaaaga caaagagttg tatggttatt
     6661 ctttttattt ttttgcgtct tgtgaggact ttggagattt agactattaa attttgtgtt
     6721 ttagggaggg cttttgattc taggtctact tgcaattgtt acacatggtt tttgtttcga
     6781 ttattccaaa gtattgagat atttatgaaa cgaaacataa gaagatatga agatgagttt
     6841 acatatacgt atacgcatat ttcaaaaaaa caaaagataa cgtattgatc ataaccactt
     6901 taactttaat ttacgagcaa tatataaata tatctataaa taggtgctag agatggtgtg
     6961 ttgaggtatt tacatattta tagggaaaac aaaaaggggg atctacattt gaggctagtt
     7021 agacttagat ttaatcttga gaaataagta actctccacc cctccaccac acccctattc
     7081 tattctgtca tttcaaccca cactttgaaa aaggataatt atatttccta acattccttt
     7141 gcgtgtttac caagagaaca atggaaaaga atgataacaa gacgaacggc acggagaatg
     7201 tggaagaata aaaccaccct ccctttcaat atataaattt ataaaacaga aaacagaaat
     7261 aaacttattg tattcaaaaa aaaactactt cataacattt gtttttattt taaagtgata
     7321 atgaaacaga ctaaattcaa acttgaactt aaatggagat ttcttttttt ttcttttttt
     7381 tggaatttct tttggttatt gttgtattca tccctttaat tggggattga tagtgaaaac
     7441 caaattccaa ttccaattcc aatcactttt aaatatattg ttgttaaaca aaacaatata
     7501 gaagagtaga ataaaggaaa gaggtggctg taattctgtt tggtgatcat gatgataagg
     7561 cccgggaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     7621 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7681 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7741 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7801 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7861 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7921 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7981 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8041 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8101 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8161 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8221 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8281 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8341 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8401 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8461 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8521 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8581 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8641 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8701 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8761 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8821 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8881 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8941 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9001 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9061 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9121 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9181 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9241 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9301 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9361 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9421 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9481 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9541 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9601 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9661 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9721 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9781 tccgcgcaca tttccccgaa aagtgccac
//
