LOCUS       40924_33637        6524 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pNUS-SPnH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Tetaud E, Lecuix I, Sheldrake T, Baltz T, Fairlamb AH.
  TITLE     A new expression vector for Crithidia fasciculata and Leishmania
  JOURNAL   Mol. Biochem. Parasitol. 120 (2), 195-204 (2002)
  PUBMED    11897125
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Tetaud E, Leciux I, Sheldrake T, Baltz T, Fairlamb AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2001) Parasitologie Moleculaire, UMR CNRS 5016, 
            146 Rue Leo Saignat, Bordeaux 33076, France
REFERENCE   3  (bases 1 to 6524)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6524)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biochem. Parasitol."; date: "2002"; volume: "120"; issue: "2"; 
            pages: "195-204"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-SEP-2001) Parasitologie Moleculaire, UMR CNRS 5016, 146 Rue Leo 
            Saignat, Bordeaux 33076, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1726..2748
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIGKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRLSTRPRAK
                     E"
     primer_bind     complement(3905..3921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3929..3945)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3953..3983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3998..4019)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4307..4895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5069..5926)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5927..6031)
                     /label=AmpR promoter
     primer_bind     6505..6521
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttaccg acaagaccag aatagcgctt atgtgtgtgt gtttgtgtgt gggtgtgtgt
       61 gcccatgtgt gacaaagagg ccagtgcttt ctctaaagca agagcgcgag gttgctggct
      121 aaggactcta agtccctcac agtgatggcg tgttgcattt tctaaaccgt caatccaaca
      181 cgtcggtagc atatcggttt tgtaggctct tctggatacc ttccccagta agaccgccac
      241 tgcttcgtga ctgaagcact gtaaatatca ctgaaaccgt cgcgttgctt tgtctaatta
      301 tcaccgcctt tgccagctat cgcctcaggc tgcgctcttt gactaatcca ctcaccactt
      361 ctctgccttc ttcctccggt tgttgttgtg tcctgcgtgt acatggcgcg tgtccttttc
      421 gagcaaacag ctgtcttttc tttcacgata acacactcat attaaacgcg agtattactc
      481 atcagtcaac gtcacattcc gctctgtcca cttcgacctt acacctctac ttgtccaact
      541 atcttccact tgtcaagcga attcatggct cgagctcgtt tccttcagct tctgctggtg
      601 acgctgacgc tcctcagtac tgctgcactc cctgtcagcg cgcatatgga tatcggtacc
      661 gaattctgta ttacgccgtt ttaagagctc cttcgacgcc tttttgtcct tcagctgctc
      721 cacggtgact cgcttctctc tctttccaca gtgtctcttt tttcttttca ctctctatac
      781 aaatgtgagc gacctccttt tctgtacaac ggccttccgg cgtgtgcttt tctcatcgac
      841 ctctcctcgc ttcttgggca ctccttcatc gagcaaacaa gacgagggag gtgacgcgat
      901 tggtgagacc actacgagta tggacggagc tgtctgatac cccctgtttt ttatcttgta
      961 ccccgctctg cagtgacggt agcccgctgc tgtgcttcgg tatcgccgct tcatacgctt
     1021 ctctcttttt tcaacgtgcg gcgctgattc aaaggttacc tcaacgcgac ccgccgctgc
     1081 atcctttgtt gctcctcttg tcgcaaacaa acaaaaaacg tgctgtgctt tttcctttat
     1141 cgtgtctttt tgcaaagtct agagtttacc gacaagacca gaatagcgct tatgtgtgtg
     1201 tgtttgtgtg tgggtgtgtg tgcccatgtg tgacaaagag gccagtgctt tctctaaagc
     1261 aagagcgcga ggttgctggc taaggactct aagtccctca cagtgatggc gtgttgcatt
     1321 ttctaaaccg tcaatccaac acgtcggtag catatcggtt ttgtaggctc ttctggatac
     1381 cttccccagt aagaccgcca ctgcttcgtg actgaagcac tgtaaatatc actgaaaccg
     1441 tcgcgttgct ttgtctaatt atcaccgcct ttgccagcta tcgcctcagg ctgcgctctt
     1501 tgactaatcc actcaccact tctctgcctt cttcctccgg ttgttgttgt gtcctgcgtg
     1561 tacatggcgc gtgtcctttt cgagcaaaca gctgtctttt ctttcacgat aacacactca
     1621 tattaaacgc gagtattact catcagtcaa cgtcacattc cgctctgtcc acttcgacct
     1681 tacacctcta cttgtccaac tatcttccac ttgtcaagcg tcgacatgaa aaagcctgaa
     1741 ctcaccgcga cgtctgtcga gaagtttctg atcggaaagt tcgacagcgt ctccgacctg
     1801 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     1861 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     1921 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga gttcagcgag
     1981 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     2041 accgaactgc ccgctgttct tcagccggtc gcggaggcca tggatgcgat cgctgcggcc
     2101 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     2161 acatggcgtg atttcatttg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     2221 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     2281 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     2341 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     2401 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     2461 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     2521 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2581 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2641 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2701 gccgatagtg gaaaccgacg cctcagcact cgtccgaggg caaaggaata gggatccagc
     2761 aggcggagaa agagggaagt ataaggcgga cgcataggtc ggagtatcga gaaaaagagg
     2821 cagagatggg tggtggcgga gcgcccctct ctgcccttct ctgttttact gttcccgcca
     2881 cgtcgccaac tcctcttttt gtttgctcta atgtcggtgc tatcctcttc ctctttttac
     2941 tctccgtttt tcttccttcc gttttgtctt gttatcaccg ttatttttct tcttctttct
     3001 tagccgattt gggtctcctg cctacggcag cggtgatgag tcgcacttcc gtcccccttt
     3061 cctctccgta agtactccct cgatgcctca ggcgcttcta ttttgcggca ctgtgctgac
     3121 cacctcccac gtgtgcagtg agagcgccag agacattcag gagaagaggg aagaggggaa
     3181 gtaaatacca aagcgaggaa gatgtctttc tcgctgcttc tcatcctgtt gaccggtgtg
     3241 cacggcggtg tgtcctctcg gcttccctcc tccttccctc gctccccttt ttctgtgttt
     3301 tttctttcta acatgattgc gcctgctctt tttgccccgc agcgtcgcag tgggtgtcac
     3361 ctgcgaccgc cgtacgtttt actatataca tatttgtttc tgtgctgatg ccatttgtcc
     3421 gctactccat atcgtgcgtg tttgttttcc agtggtgacg ccttccgcat cattttaatc
     3481 aattcgaggc gaaaagggtg atttgagtgt tggagtgggc ttttttattt atttgactcc
     3541 aaacatcttt tcttttcccg gtatcttcgg gtccgtcacg caggtcggtg ggtgtgtccg
     3601 agtgcgcttc tttactcacg tgccgctgcg cacatacaca gattttaaaa gcacgcatac
     3661 acgatctgtg ccttcaaaac taatcaacaa aaacaaaaat acaaaaaaga caagaataag
     3721 aggtcagcaa cgcacccacg gctcctttct tcctgatcca cgtcgtggcc gctgtacgct
     3781 ctcaaacacg ctttggcgct gatgcgtgcc tttatacaaa gaacaaagaa gaagcgaaaa
     3841 acgggagcgt gttgctttgg ggatgtatgc gtgccacagt tctgcggtac aattcgtaat
     3901 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3961 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4021 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4081 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4141 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4201 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4261 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4501 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4741 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4921 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4981 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5041 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5101 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5161 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5221 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5281 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5341 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5401 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5461 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5521 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5581 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     5641 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5701 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5761 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5821 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5881 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5941 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6001 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6061 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     6121 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6181 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6241 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6301 gagtgcacga tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6361 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6421 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6481 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgcc
//
