LOCUS       40924_33572        5608 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTKV1901, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Skinner H, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5608)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5608)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        236..298
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           320..359
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             377..1504
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     polyA_signal    1549..1597
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2027..2043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(2132..2356)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2397..3197)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(3211..3711)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      3789..3798
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        3842..3946
                     /label=AmpR promoter
     CDS             3947..4804
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4978..5566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cggaccgtag agtcgagcag tgtggttttc aagaggaagc aaaaagcctc tccacccagg
       61 cctggaatgt ttccacccaa tgtcgagcag tgtggttttg caagaggaag caaaaagcct
      121 ctccacccag gcctggaatg tttccaccca atgtcgagca aaccccgccc agcgtcttgt
      181 cattggcgaa ttggaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc
      241 atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca
      301 gcgtcaacag cgtgccgcac atcttggtgg cgtgaaactc ccgcacctct tcggccagcg
      361 ccttgtagaa gcgcgtatgg cttcgtaccc cggccatcag cacgcgtctg cgttcgacca
      421 ggctgcgcgt tctcgcggcc atagcaaccg acgtacggcg ttgcgccctc gccggcagca
      481 agaagccacg gaagtccgcc cggagcagaa aatgcccacg ctactgcggg tttatataga
      541 cggtccccac gggatgggga aaaccaccac cacgcaactg ctggtggccc tgggttcgcg
      601 cgacgatatc gtctacgtac ccgagccgat gacttactgg cgggtgctgg gggcttccga
      661 gacaatcgcg aacatctaca ccacacaaca ccgccttgac cagggtgaga tatcggccgg
      721 ggacgcggcg gtggtaatga caagcgccca gataacaatg ggcatgcctt ataccgtgac
      781 cgacgccgtt ctggctcctc atatcggggg ggaggctggg agctcacatg ccccgccccc
      841 ggccctcacc ctcatcttcg accgccatcc catcgccgcc ctcctgtgct acccggccgc
      901 gcgatacctt atgggcagca tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc
      961 gccgaccttg cccggcacaa acatcgtgtt gggggccctt ccggaggaca gacacatcga
     1021 ccgcctggcc aaacgccagc gccccggcga gcggcttgac ctggctatgc tgaccgcgat
     1081 tcgccgcgtt tacgggctgc ttgccaatac ggtgcggtat ctgcagggcg gcgggtcgtg
     1141 gcgggaggat tggggacagc tttcggggac ggccgtgccg ccccagggtg ccgagcccca
     1201 gagcaacgcg ggcccacgac cccatatcgg ggacacgtta tttaccctgt ttcgggcccc
     1261 cgagttgctg gcccccaacg gcgacctgta caacgtgttt gcctgggcct tggacgtctt
     1321 ggccaaacgc ctccgtccca tgcacgtctt tatcctggat tacgaccaat cgcccgccgg
     1381 ctgccgggac gccctgctgc aacttacctc cgggatgatc cagacccacg tcaccacccc
     1441 aggctccata ccgacgatct gcgacctggc gcgcacgttt gcacgggaga tgggggaggc
     1501 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgtcg gcaataaaaa
     1561 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     1621 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     1681 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     1741 gggcggcagg cctgccatag ccacgggccc cgtgggttag ggacggggtc ccccatgggg
     1801 aatggtttat ggttcgtggg ggttattatt ttgggcgttg cgtggggtca gtccacgact
     1861 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
     1921 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
     1981 gcggatttct ggcgccgccg gacgaactaa acctgactac ggaccggtaa aacgacggcc
     2041 agtgttaact ctaggagatc ttctaggaag ctttctaggg tacctctagc tcgaggggcg
     2101 cgcccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc
     2161 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     2221 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     2281 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     2341 caactagaag gcacagtcga ggctgatcag cgagctctag agaattgatc ccctcagaag
     2401 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     2461 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     2521 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     2581 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     2641 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     2701 tgatgctctt cgtccagatc atcctgattg acaagaccgg cttccatccg agtacgtgct
     2761 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     2821 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     2881 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     2941 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     3001 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     3061 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     3121 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     3181 tcaatggccg atcccatatt ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa
     3241 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcttccgg aggaccttcg
     3301 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
     3361 cctctgagcc cagaaagcga aggagccaaa gctgctattg gccgctgccc caaaggccta
     3421 cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgaga cgtgctactt
     3481 ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg
     3541 gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg
     3601 tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg
     3661 ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagggcg
     3721 cgccgggatc cctagaatcg atcctagaga attcctagac cgcggctaga cccggggggg
     3781 tcgacgcggc cgccatggtc atagctgttt gacgtcaggt ggcacttttc ggggaaatgt
     3841 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3961 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4021 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4081 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4141 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4201 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4261 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4321 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4381 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4441 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4501 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4561 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4621 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4681 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4741 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4801 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4861 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5101 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5341 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     5401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5581 ggccttttta cggttcctgg ccttttgc
//
