LOCUS       40924_33537        3194 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Brucella expression vector pNSVirB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Seleem MN, Jain N, Alqublan H, Vemulapalli R, Boyle SM, 
            Sriranganathan N.
  TITLE     Activity of native vs. synthetic promoters in Brucella
  JOURNAL   FEMS Microbiol. Lett. 288 (2), 211-215 (2008)
  PUBMED    18811654
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Seleem MN, Sriranganathan N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA
REFERENCE   3  (bases 1 to 3194)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3194)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2008"; volume: "288"; issue: "2"; pages: 
            "211-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             508..525
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        689..791
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             792..1424
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQF"
     CDS             complement(1542..2201)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2202..2971)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 atgccgcctt gttcaccggc tagctgaaat ccaggcgttg agatcgccat gacaggcata
       61 tttcaacgcg acatattcat ttgctaccgc atttagaccc ttcgcggcca acaccacttt
      121 tcctgatgcc gcctaatgga gcaagttcgg taaaaattta gccagcttgg atgtctgcca
      181 attatgtaga gcgattcttg gtcgggttac aaccatggca aagggcctct tgtctcatgc
      241 aaggcgcgcc ggcacccgca aaaatatgaa aatcaccgca taccacttgt atataagatt
      301 ttgttaaaaa agaattttct aatagaacca atacaagtgc cccaaaattc tgactgggcg
      361 tcattcacac aatatatagc cccctcacaa gcatatttgt ccatatatcg gcttaaacaa
      421 cactacccaa taatgaccga tatcgctgat ctataattaa ggctatcata ataatcgtcc
      481 attctctgag aaggagacga tcctatgcat catcatcatc atcatggtgg atccgagctc
      541 gagatctgca gctggtacca tggaattcga agcttgggtc tagagaataa atacctgtga
      601 cggaagatca cttcgcagaa taaataaatc ctggtgtccc tgttgatacc gggaagccct
      661 gggccaactt ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
      721 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      781 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      841 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      901 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      961 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     1021 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     1081 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     1141 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1201 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     1261 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     1321 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     1381 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtttttatgc atgcgcccaa
     1441 tacgcaaacc gcctctcccc gcgcgttggc cgaaagtaat tacgtcgtga tctatagtct
     1501 ggaacagcgc acttacgggt tgctgcgcaa cccaagtgct accggcgcgg cagcgtgacc
     1561 cgtgtcggcg gctccaacgg ctcgccatcg tccagaaaac acggctcatc gggcatcggc
     1621 aggcgctgct gcccgcgccg ttcccattcc tccgtttcgg tcaaggctgg caggtctggt
     1681 tccatgcccg gaatgccggg ctggctgggc ggctcctcgc cggggccggt cggtagttgc
     1741 tgctcgcccg gatacagggt cgggatgcgg cgcaggtcgc catgccccaa cagcgattcg
     1801 tcctggtcgt cgtgatcaac caccacggcg gcactgaaca ccgacaggcg caactggtcg
     1861 cggggctggc cccacgccac gcggtcattg accacgtagg ccgacacggt gccggggccg
     1921 ttgagcttca cgacggagat ccagcgctcg gccaccaagt ccttgactgc gtattggacc
     1981 gtccgcaaag aacgtccgat gagcttggaa agtgtcttct ggctgaccac cacggcgttc
     2041 tggtggccca tctgcgccac gaggtgatgc agcagcattg ccgccgtggg tttcctcgca
     2101 ataagcccgg cccacgcctc atgcgctttg cgttccgttt gcacccagtg accgggcttg
     2161 ttcttggctt gaatgccgat ttctctggac tgcgtggcca tgcttatctc catgcggtag
     2221 ggtgccgcac ggttgcggca ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac
     2281 aattatgcgt tgcgtaaaag tggcagtcaa ttacagattt tctttaacct acgcaatgag
     2341 ctattgcggg gggtgccgca atgagctgtt gcgtaccccc cttttttaag ttgttgattt
     2401 ttaagtcttt cgcatttcgc cctatatcta gttctttggt gcccaaagaa gggcacccct
     2461 gcggggttcc cccacgcctt cggcgcggct ccccctccgg caaaaagtgg cccctccggg
     2521 gcttgttgat cgactgcgcg gccttcggcc ttgcccaagg tggcgctgcc cccttggaac
     2581 ccccgcactc gccgccgtga ggctcggggg gcaggcgggc gggcttcgcc ttcgactgcc
     2641 cccactcgca taggcttggg tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc
     2701 tgcgcgagcc ttgacccgcc ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc
     2761 gcaggccgga ggcttttccc cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc
     2821 cgcgcagttg gagccggtgg gtatgtggtc gaaggctggg tagccggtgg gcaatccctg
     2881 tggtcaagct cgtgggcagg cgcagcctgt ccatcagctt gtccagcagg gttgtccacg
     2941 ggccgagcga agcgagccag ccggtggccg ctcgcggcca tcgtccacat atccacgggc
     3001 tggcaaggga gcgcagcgac cgcgcagggc gaagcccgga gagcaagccc gtagggcgcc
     3061 gcagccgccg taggcggtca cgactttgcg aagcaaagtc tagtgagtat actcaagcat
     3121 tgagtggccc gccggaggca ccgccttgcg ctgcccccgt cgagccggtt ggacaccaaa
     3181 agggaggggc aggc
//
