LOCUS       40924_33532        3022 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Brucella expression vector pNSHtrA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Seleem MN, Jain N, Alqublan H, Vemulapalli R, Boyle SM, 
            Sriranganathan N.
  TITLE     Activity of native vs. synthetic promoters in Brucella
  JOURNAL   FEMS Microbiol. Lett. 288 (2), 211-215 (2008)
  PUBMED    18811654
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Seleem MN, Sriranganathan N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA
REFERENCE   3  (bases 1 to 3022)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2008"; volume: "288"; issue: "2"; pages: 
            "211-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             336..353
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        517..619
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             620..1252
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQF"
     CDS             complement(1370..2029)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2030..2799)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 aattcgttct tctcaaaccc cgtttcaacg cgcagacggc gctgctttgg ggctttcctg
       61 ttattattct cctgattggc ggcggcgcgc ttcttgttgc tttccggcgt gcggcgcgct
      121 gctggcacag ccccacagcc ccttccgaaa gcgaaaaagc ggaactttcc cgcctgctcg
      181 acggaaaata ggccaacaaa acctctggaa accttacgaa actttcatct tgcggaaatt
      241 gaacggtaag gtagctctaa ctatcttgct ctcatagaaa attacgaggc cagaaattcc
      301 tggcccggat tactgctcgc aaggagaaac agatgcatca tcatcatcat catggtggat
      361 ccgagctcga gatctgcagc tggtaccatg gaattcgaag cttgggtcta gagaataaat
      421 acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg
      481 gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      541 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      601 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      661 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      721 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      781 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      841 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      901 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      961 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1021 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     1081 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1141 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1201 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt ttttatgcat
     1261 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg aaagtaatta cgtcgtgatc
     1321 tatagtctgg aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca
     1381 gcgtgacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg
     1441 gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca
     1501 ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg
     1561 gtagttgctg ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca
     1621 gcgattcgtc ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca
     1681 actggtcgcg gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc
     1741 cggggccgtt gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt
     1801 attggaccgt ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca
     1861 cggcgttctg gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt
     1921 tcctcgcaat aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac
     1981 cgggcttgtt cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca
     2041 tgcggtaggg tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc
     2101 gcgacaacaa ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac
     2161 gcaatgagct attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt
     2221 gttgattttt aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg
     2281 gcacccctgc ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc
     2341 cctccggggc ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc
     2401 cttggaaccc ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt
     2461 cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc
     2521 gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg
     2581 cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg
     2641 ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc
     2701 aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt
     2761 tgtccacggg ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat
     2821 ccacgggctg gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt
     2881 agggcgccgc agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac
     2941 tcaagcattg agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg
     3001 acaccaaaag ggaggggcag gc
//
