LOCUS       40924_33527        3148 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Brucella expression vector pNSClpB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Seleem MN, Jain N, Alqublan H, Vemulapalli R, Boyle SM, 
            Sriranganathan N.
  TITLE     Activity of native vs. synthetic promoters in Brucella
  JOURNAL   FEMS Microbiol. Lett. 288 (2), 211-215 (2008)
  PUBMED    18811654
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Seleem MN, Sriranganathan N.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA
REFERENCE   3  (bases 1 to 3148)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3148)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2008"; volume: "288"; issue: "2"; pages: 
            "211-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2008) ICTAS, Virginia Tech, 1410 Prices Fork Rd., 
            Blacksburg, VA 24061, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             462..479
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        643..745
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             746..1378
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQF"
     CDS             complement(1496..2155)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(2156..2925)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 tcggcagcga agaccgtgct gacgacaagc aggaagaaaa ggccgaacag ccgcagccgg
       61 ctatagaagc accggccgca gccgccaggg ttgaggaagc agctccggct gttgtcgaag
      121 cagcccctgt cgaggcttct gcggaagaag ctggcgcacc gcggcgcgcc acacgccgtc
      181 ccggccgtcc acgccgcacc gctgccaagg atagcggtga cgatacgccg gttgcggaag
      241 cagccgattc cgacgcataa gtttccagct tcgataaaaa ctttgagatg gcgcggtttt
      301 ccgcgccatt tttatttgga ttttccgatg cctgccggtc ttgccgaaag agggcaatat
      361 tcccatatgc acttttgaga agagcccgcc cttgtggggg gtcgtcaccc aacatctctc
      421 ggtgccgcgg gacggaccga gaggcaaacg gagacattat gcatcatcat catcatcatg
      481 gtggatccga gctcgagatc tgcagctggt accatggaat tcgaagcttg ggtctagaga
      541 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
      601 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
      661 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      721 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      781 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      841 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      901 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      961 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     1021 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     1081 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     1141 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     1201 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     1261 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1321 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagttttt
     1381 atgcatgcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgaaag taattacgtc
     1441 gtgatctata gtctggaaca gcgcacttac gggttgctgc gcaacccaag tgctaccggc
     1501 gcggcagcgt gacccgtgtc ggcggctcca acggctcgcc atcgtccaga aaacacggct
     1561 catcgggcat cggcaggcgc tgctgcccgc gccgttccca ttcctccgtt tcggtcaagg
     1621 ctggcaggtc tggttccatg cccggaatgc cgggctggct gggcggctcc tcgccggggc
     1681 cggtcggtag ttgctgctcg cccggataca gggtcgggat gcggcgcagg tcgccatgcc
     1741 ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac ggcggcactg aacaccgaca
     1801 ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc attgaccacg taggccgaca
     1861 cggtgccggg gccgttgagc ttcacgacgg agatccagcg ctcggccacc aagtccttga
     1921 ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga
     1981 ccaccacggc gttctggtgg cccatctgcg ccacgaggtg atgcagcagc attgccgccg
     2041 tgggtttcct cgcaataagc ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc
     2101 agtgaccggg cttgttcttg gcttgaatgc cgatttctct ggactgcgtg gccatgctta
     2161 tctccatgcg gtagggtgcc gcacggttgc ggcaccatgc gcaatcagct gcaacttttc
     2221 ggcagcgcga caacaattat gcgttgcgta aaagtggcag tcaattacag attttcttta
     2281 acctacgcaa tgagctattg cggggggtgc cgcaatgagc tgttgcgtac cccccttttt
     2341 taagttgttg atttttaagt ctttcgcatt tcgccctata tctagttctt tggtgcccaa
     2401 agaagggcac ccctgcgggg ttcccccacg ccttcggcgc ggctccccct ccggcaaaaa
     2461 gtggcccctc cggggcttgt tgatcgactg cgcggccttc ggccttgccc aaggtggcgc
     2521 tgcccccttg gaacccccgc actcgccgcc gtgaggctcg gggggcaggc gggcgggctt
     2581 cgccttcgac tgcccccact cgcataggct tgggtcgttc caggcgcgtc aaggccaagc
     2641 cgctgcgcgg tcgctgcgcg agccttgacc cgccttccac ttggtgtcca accggcaagc
     2701 gaagcgcgca ggccgcaggc cggaggcttt tccccagaga aaattaaaaa aattgatggg
     2761 gcaaggccgc aggccgcgca gttggagccg gtgggtatgt ggtcgaaggc tgggtagccg
     2821 gtgggcaatc cctgtggtca agctcgtggg caggcgcagc ctgtccatca gcttgtccag
     2881 cagggttgtc cacgggccga gcgaagcgag ccagccggtg gccgctcgcg gccatcgtcc
     2941 acatatccac gggctggcaa gggagcgcag cgaccgcgca gggcgaagcc cggagagcaa
     3001 gcccgtaggg cgccgcagcc gccgtaggcg gtcacgactt tgcgaagcaa agtctagtga
     3061 gtatactcaa gcattgagtg gcccgccgga ggcaccgcct tgcgctgccc ccgtcgagcc
     3121 ggttggacac caaaagggag gggcaggc
//
