LOCUS       62056_18220        5220 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Cloning vector pNRVL-caSAT1, complete sequence.
ACCESSION   MN989861
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1094..1591
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     gene            1592..2818
                     /gene="caSAT1"
                     /label=caSAT1
     CDS             join(1592..1601,2256..2818)
                     /codon_start=1
                     /transl_table=11
                     /gene="caSAT1"
                     /product="streptothricin acetyltransferase"
                     /label=caSAT1
                     /note="codon-optimized for Candida albicans"
                     /protein_id="QID92292.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     regulatory      2822..2951
                     /label=Candida albicans URA3 terminator
                     /note="Candida albicans URA3 terminator"
                     /regulatory_class="terminator"
     misc_feature    2979..2984
                     /label=BssHII site for insert ligation
                     /note="BssHII site for insert ligation"
     misc_feature    2992..2997
                     /label=XhoI site for insert ligation
                     /note="XhoI site for insert ligation"
     misc_feature    2998..3448
                     /note="site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     misc_feature    3449..3454
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     promoter        complement(3462..3480)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3485..3501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3614..4420
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4570..5158
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcc gagcgtcaaa actagagaat aataaagaaa acgatctttt caaaaagaaa
     1141 aaacctttta gttttccttt gttgttgttg tgggtgtgtg ctatttatat tatatagttt
     1201 actcataata ccataaaata ttcggtttga ttaggttatt ttaataagct aatttgtttc
     1261 taatcgtgta atttatgctg tgtatattaa gtagtgtgtg cactgcccaa aaatgtttgt
     1321 tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc atacacacac gttaattagt
     1381 tgttcaacgt aatacactca tattttgttc ttatttgctt tcggtcgctg ttctcaccaa
     1441 gatttattgc caacgaaaca attttttttt atatattttc agatttttct ttttttcctt
     1501 tcctttcctt ttctaatttt cactcctggt tttctttctt tcttagaaac attatctcga
     1561 tattaatatt aaaaaaatat aatcattcaa aatggacggt ggtatgtttt agtttagctt
     1621 caattctaat tgattgatta atcagttgat tggtttcaat atgacaaatg ggtagggtgg
     1681 gaaaacttca ttttcaattc agatcaaact tttttgttgt cgacataata tttctcgttt
     1741 gggatgttac tgtcacatta ataatacaca cacatcagct tataattttg aaagtaattt
     1801 atcagatatg ttgtgacgat cagtggaaat ggctaacttc aatgtatctg ttcttcccct
     1861 ttttcaaagt tcacgttttt tgattgattg attgatctgt cggcagtggt ttcaaaacca
     1921 ttcggtgagt aatcctatca atcaatgtta cgacaaaagg ctcaatattc aaaattgcaa
     1981 tgttttatgt tttcctacgt gtacttgtgc aaggcaattg attcaacatt gcttttggtg
     2041 tttgacgagt ttctagtttg gacttgtgtt gttatctggg ctatacagat ttcccggctc
     2101 actatgaatt ttttttttcg acgctcagtg cacacaacta taaacaacac aaacacaaac
     2161 acagcaagaa aaaaaaaaaa cgaacattga attgaaacca agccaactga aaaattcctt
     2221 atttaaatga ctgtcatact aacccatttt tatagaagaa gttgctgctt tagttatcga
     2281 taacggttct catatgaaaa tttcggtgat ccctgagcag gtggcggaaa cattggatgc
     2341 tgagaaccat ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga
     2401 actatctacc agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga
     2461 tgaagactct gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact
     2521 caactcaaca tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg
     2581 aggcaaagga gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca
     2641 gctccttggc atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc
     2701 aaaatgtggc tttactctcg gcggcattga cctcttcacg tataaaacta gacctcaagt
     2761 ctcgaacgaa acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca
     2821 tatgtgaagt gtgaaggggg agattttcac tttattagat ttgtatatat gtataataaa
     2881 taaataaata agttaaataa ataattagat aagggtggta attattacta tttacaatca
     2941 aaggtggtcc tggaattctg cagatatcca tcacactggc gcgctacgga tctcgagcga
     3001 agtagtcctc actgaggaga tcccaggcta gaaatcagca aattttacat ccctcatatt
     3061 ctgacacgag tttaactaca tagcaattgt aaaagggctc ttgttttttt tttttttttg
     3121 tggcggtgca gtacgtacac gatctttaaa gattagggct ataaccatgt caaagtgact
     3181 catttttttt gttcaaatgc gcactcggat ttaaagcagc aaaaaaatac ttcgttacaa
     3241 aaatggaagc gcattttccg aaaaaggtta attcctagcg attttattat ttttgtttta
     3301 tttattaatt tttcttgttt tgctattgca tttttgcaac caaaaataga aaattctcgt
     3361 ttttttttgt tgtagcgcaa ccaatttctg gttattcttt tttttttgca atttttaaat
     3421 ttgcacgtga cagtgttagt gttagtgcag gcctgatatc ccctatagtg agtcgtatta
     3481 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     3541 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     3601 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     3661 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3721 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3781 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3841 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3901 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3961 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     4021 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     4081 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     4141 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     4201 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     4261 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     4321 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     4381 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     4441 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     4501 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4561 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4621 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4681 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4741 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4801 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4861 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4921 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4981 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5041 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5101 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5161 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
