LOCUS       62056_18215        7111 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Expression vector pNRVL-caSAT1-GFP, complete sequence.
ACCESSION   MT001912
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1094..1591
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     gene            1592..2818
                     /gene="caSAT1"
                     /label=caSAT1
     CDS             join(1592..1601,2256..2818)
                     /codon_start=1
                     /transl_table=11
                     /gene="caSAT1"
                     /product="streptothricin acetyltransferase"
                     /label=caSAT1
                     /note="codon-optimized for Candida albicans"
                     /protein_id="QID92297.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     regulatory      2822..2951
                     /label=Candida albicans URA3 terminator
                     /note="Candida albicans URA3 terminator"
                     /regulatory_class="terminator"
     regulatory      complement(2999..3239)
                     /label=Saccharomyces cerevisiae URA3 terminator
                     /note="Saccharomyces cerevisiae URA3 terminator"
                     /regulatory_class="terminator"
     protein_bind    3260..3284
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3288..4001)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     protein_bind    complement(4002..4026)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      complement(4033..4882)
                     /label=Candida albicans TDH3 promoter
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     misc_feature    4889..5339
                     /note="site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     misc_feature    5340..5345
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     promoter        complement(5353..5371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5376..5392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5505..6311
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6461..7049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcc gagcgtcaaa actagagaat aataaagaaa acgatctttt caaaaagaaa
     1141 aaacctttta gttttccttt gttgttgttg tgggtgtgtg ctatttatat tatatagttt
     1201 actcataata ccataaaata ttcggtttga ttaggttatt ttaataagct aatttgtttc
     1261 taatcgtgta atttatgctg tgtatattaa gtagtgtgtg cactgcccaa aaatgtttgt
     1321 tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc atacacacac gttaattagt
     1381 tgttcaacgt aatacactca tattttgttc ttatttgctt tcggtcgctg ttctcaccaa
     1441 gatttattgc caacgaaaca attttttttt atatattttc agatttttct ttttttcctt
     1501 tcctttcctt ttctaatttt cactcctggt tttctttctt tcttagaaac attatctcga
     1561 tattaatatt aaaaaaatat aatcattcaa aatggacggt ggtatgtttt agtttagctt
     1621 caattctaat tgattgatta atcagttgat tggtttcaat atgacaaatg ggtagggtgg
     1681 gaaaacttca ttttcaattc agatcaaact tttttgttgt cgacataata tttctcgttt
     1741 gggatgttac tgtcacatta ataatacaca cacatcagct tataattttg aaagtaattt
     1801 atcagatatg ttgtgacgat cagtggaaat ggctaacttc aatgtatctg ttcttcccct
     1861 ttttcaaagt tcacgttttt tgattgattg attgatctgt cggcagtggt ttcaaaacca
     1921 ttcggtgagt aatcctatca atcaatgtta cgacaaaagg ctcaatattc aaaattgcaa
     1981 tgttttatgt tttcctacgt gtacttgtgc aaggcaattg attcaacatt gcttttggtg
     2041 tttgacgagt ttctagtttg gacttgtgtt gttatctggg ctatacagat ttcccggctc
     2101 actatgaatt ttttttttcg acgctcagtg cacacaacta taaacaacac aaacacaaac
     2161 acagcaagaa aaaaaaaaaa cgaacattga attgaaacca agccaactga aaaattcctt
     2221 atttaaatga ctgtcatact aacccatttt tatagaagaa gttgctgctt tagttatcga
     2281 taacggttct catatgaaaa tttcggtgat ccctgagcag gtggcggaaa cattggatgc
     2341 tgagaaccat ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga
     2401 actatctacc agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga
     2461 tgaagactct gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact
     2521 caactcaaca tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg
     2581 aggcaaagga gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca
     2641 gctccttggc atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc
     2701 aaaatgtggc tttactctcg gcggcattga cctcttcacg tataaaacta gacctcaagt
     2761 ctcgaacgaa acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca
     2821 tatgtgaagt gtgaaggggg agattttcac tttattagat ttgtatatat gtataataaa
     2881 taaataaata agttaaataa ataattagat aagggtggta attattacta tttacaatca
     2941 aaggtggtcc tggaattctg cagatatcca tcacactggc gcgtgaattc gatctgctat
     3001 gcgtccatct ttacagtcct gtcttattgt tcttgatttg tgccccgtaa aatactgtta
     3061 cttggttctg gcgaggtatt ggatagttcc tttttataaa ggccatgaag ctttttcttt
     3121 ccaatttttt tttttcgtca ttatagaaat cattacgacc gagattcccg ggtaataact
     3181 gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt acttataatg
     3241 ctatttattc atgctagcaa ccactttgta caagaaagct gggtttattt gtacaattca
     3301 tccataccat gggtaatacc agcagcagta acaaattcta acaagaccat gtggtctctc
     3361 ttttcgtttg gatctttgga taaggcagat tgagtggata agtaatggtt gtctggtaac
     3421 aagactggac catcaccaat tggagtattt tgttgataat ggtcagctaa ttgaacagaa
     3481 ccatcttcaa tgttgtgtct aattttgaag ttaactttga taccattctt ttgtttgtca
     3541 gccatgatgt aaacattgtg agagttatag ttgtattcca atttgtgacc taaaatgtta
     3601 ccatcttctt taaaatcaat accttttaat tcgattctat taactaaggt atcaccttca
     3661 aacttgactt cagctctggt cttgtagtta ccgtcatctt tgaaaaaaat agttctttct
     3721 tgaacataac cttctggcat ggcagacttg aaaaagtcat gttgtttcat atgatctggg
     3781 tatctcgcaa aacattgaac accataaccg aaagtagtga ctaaggttgg ccatggaact
     3841 ggcaatttac cagtagtaca aataaatttt aaggtcaatt taccgtaagt agcatcacct
     3901 tcaccttcac cggagacaga aaatttgtga ccattaacat caccatctaa ttcaaccaaa
     3961 attgggacaa caccagtgaa taattcttca cctttagaca tagcctgctt ttttgtacaa
     4021 acttgttgaa tcgattgtaa agtttgttga tgttaattga tttgaattca attgtgatga
     4081 ataaaagaaa gaaaaagaaa aagaaaaaaa gaaagaagaa ataaataaat taaaaaaatt
     4141 ttccaattga agggaatttc aagggttaat atatattaag tcaaaaaaaa tccaattttc
     4201 gttggaattg gctttgacaa gacaaatttt agtggttgag tgggttggtt gaaacaattt
     4261 ccacctattt ttgtctattt tggctatttt tgcataatat gatttgtacg ggacacgcca
     4321 tacccggggc attatcatcc attcagaagc aattttgcgt cattatccat tttactaact
     4381 aactattaag caaagaaaag aaaaaatcat cacaacaaaa ataaaaaatg tgcaccacga
     4441 gcctggattt gaaacagctg atccaaaaac cgcaacctaa ttaattagac aatacctttc
     4501 agttaacacc gattatacct tttattgaac tcaaactagc atttgatgat actgatttat
     4561 tcaattgatg gtattatatc agtcaattgc tctgaaagca aaatatattc ggataaccga
     4621 gtgtttagag aataagcttc tacgtaaaat aataaactgc atgaaaaaaa ttaagggggt
     4681 taaagaaatt gccagccaaa atgtgagtaa atttgttata attcacggct ctccttgtaa
     4741 tttttcatgg aacactatca atttttcgga attgaaccac gcgatttgta ttttgcaagt
     4801 caaattaatc atactgaata ctgtatggtg acgttccaaa ttgaccctaa aataaaatat
     4861 cgcaattgtc tgtatgtgtg tgctcgagcg aagtagtcct cactgaggag atcccaggct
     4921 agaaatcagc aaattttaca tccctcatat tctgacacga gtttaactac atagcaattg
     4981 taaaagggct cttgtttttt tttttttttt gtggcggtgc agtacgtaca cgatctttaa
     5041 agattagggc tataaccatg tcaaagtgac tcattttttt tgttcaaatg cgcactcgga
     5101 tttaaagcag caaaaaaata cttcgttaca aaaatggaag cgcattttcc gaaaaaggtt
     5161 aattcctagc gattttatta tttttgtttt atttattaat ttttcttgtt ttgctattgc
     5221 atttttgcaa ccaaaaatag aaaattctcg tttttttttg ttgtagcgca accaatttct
     5281 ggttattctt ttttttttgc aatttttaaa tttgcacgtg acagtgttag tgttagtgca
     5341 ggcctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     5401 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     5461 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     5521 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     5581 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     5641 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     5701 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     5761 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     5821 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     5881 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     5941 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     6001 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     6061 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     6121 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     6181 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     6241 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     6301 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     6361 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     6421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7081 tggccttttg ctggcctttt gctcacatgt t
//
