LOCUS       62056_18205        7998 bp DNA     circular SYN 20-MAR-2021
DEFINITION  Cloning vector pNRVL-caSAT1-6kbp, complete sequence.
ACCESSION   MT948180
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Nemeth TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-AUG-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="Site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     misc_feature    1040..1047
                     /label=NotI site for selectable marker ligation
                     /note="NotI site for selectable marker ligation"
     regulatory      1094..1591
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     gene            join(1592..1601,2256..2818)
                     /gene="caSAT1"
                     /label=caSAT1
     CDS             join(1592..1601,2256..2818)
                     /codon_start=1
                     /transl_table=11
                     /gene="caSAT1"
                     /product="streptothricin acetyltransferase"
                     /label=caSAT1
                     /note="codon-optimized for Candida albicans"
                     /protein_id="QSX26213.1"
                     /translation="MDGEEVAALVIDNGSHMKISVIPEQVAETLDAENHFIVREVFDVH
                     LSDQGFELSTRSVSPYRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEH
                     IVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDL
                     FTYKTRPQVSNETAMYWYWFSGAQDDA"
     regulatory      2822..2951
                     /label=Candida albicans URA3 terminator
                     /note="Candida albicans URA3 terminator"
                     /regulatory_class="terminator"
     misc_feature    2979..2984
                     /note="BssHII site for selectable marker and insert
                     ligation"
     misc_feature    2985..5756
                     /note="Candida albicans intergenic region; 
                     Ca22chrRB_C_albicans_SC5314:2080135-2082906"
     misc_feature    5757..5762
                     /note="BssHII site for selectable marker and insert
                     ligation"
     misc_feature    5770..5775
                     /label=XhoI site for insert ligation
                     /note="XhoI site for insert ligation"
     misc_feature    5776..6226
                     /note="Site of recombination; CpNEUT5LDowndown from C. 
                     parapsilosis"
     promoter        complement(6240..6258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6263..6279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6392..7198
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7348..7936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcc gagcgtcaaa actagagaat aataaagaaa acgatctttt caaaaagaaa
     1141 aaacctttta gttttccttt gttgttgttg tgggtgtgtg ctatttatat tatatagttt
     1201 actcataata ccataaaata ttcggtttga ttaggttatt ttaataagct aatttgtttc
     1261 taatcgtgta atttatgctg tgtatattaa gtagtgtgtg cactgcccaa aaatgtttgt
     1321 tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc atacacacac gttaattagt
     1381 tgttcaacgt aatacactca tattttgttc ttatttgctt tcggtcgctg ttctcaccaa
     1441 gatttattgc caacgaaaca attttttttt atatattttc agatttttct ttttttcctt
     1501 tcctttcctt ttctaatttt cactcctggt tttctttctt tcttagaaac attatctcga
     1561 tattaatatt aaaaaaatat aatcattcaa aatggacggt ggtatgtttt agtttagctt
     1621 caattctaat tgattgatta atcagttgat tggtttcaat atgacaaatg ggtagggtgg
     1681 gaaaacttca ttttcaattc agatcaaact tttttgttgt cgacataata tttctcgttt
     1741 gggatgttac tgtcacatta ataatacaca cacatcagct tataattttg aaagtaattt
     1801 atcagatatg ttgtgacgat cagtggaaat ggctaacttc aatgtatctg ttcttcccct
     1861 ttttcaaagt tcacgttttt tgattgattg attgatctgt cggcagtggt ttcaaaacca
     1921 ttcggtgagt aatcctatca atcaatgtta cgacaaaagg ctcaatattc aaaattgcaa
     1981 tgttttatgt tttcctacgt gtacttgtgc aaggcaattg attcaacatt gcttttggtg
     2041 tttgacgagt ttctagtttg gacttgtgtt gttatctggg ctatacagat ttcccggctc
     2101 actatgaatt ttttttttcg acgctcagtg cacacaacta taaacaacac aaacacaaac
     2161 acagcaagaa aaaaaaaaaa cgaacattga attgaaacca agccaactga aaaattcctt
     2221 atttaaatga ctgtcatact aacccatttt tatagaagaa gttgctgctt tagttatcga
     2281 taacggttct catatgaaaa tttcggtgat ccctgagcag gtggcggaaa cattggatgc
     2341 tgagaaccat ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga
     2401 actatctacc agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga
     2461 tgaagactct gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact
     2521 caactcaaca tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg
     2581 aggcaaagga gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca
     2641 gctccttggc atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc
     2701 aaaatgtggc tttactctcg gcggcattga cctcttcacg tataaaacta gacctcaagt
     2761 ctcgaacgaa acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca
     2821 tatgtgaagt gtgaaggggg agattttcac tttattagat ttgtatatat gtataataaa
     2881 taaataaata agttaaataa ataattagat aagggtggta attattacta tttacaatca
     2941 aaggtggtcc tggaattctg cagatatcca tcacactggc gcgctgcaac tttgttctaa
     3001 atggttcgat tggattctcc caattacaca aaaattatta ttttcgtgct gttttggcgc
     3061 cgacttctat tttttaaatc ccaacaggga ttccgatcca aatcagcagg tatttctatc
     3121 tgatttcttt tcaatttggt tgcttttttc tgaacttatt tcgtattaat gggcaacaat
     3181 ttataaagat tattcagaat actagttgaa aaaattgtgt atagtaagct tcatatggaa
     3241 ttaaattgaa tgattttagt tatgtatttt taaggagttt ctttccatac tatgggatat
     3301 ggaaatatgt atattctatt tcatatccct tttcttgttt ccaatttgaa tattcaattt
     3361 ccgaaaaaca aaaaagaaca caaaacaaaa caactaataa tgatgatgtt ttcattctgt
     3421 tttgtgtaat attccttctc ctgactaata aattgatata tatggcataa tacaaaaatg
     3481 agtgaagcta aattctaatt gcatgttgca atttattcta gcctttaaaa atgtcggaat
     3541 tgagaatgtg ttgtaatgtt gtattgtatc ccttaaacta ctacaccatt acagtttttt
     3601 tttgtgttat tgtttttgaa aaaagtcaaa attagaaagt tacagggata gtttcaatag
     3661 tttctggtag gttcctccta aagttgaatt tgggtagaaa gacagccagg atccttggat
     3721 tgtttagtat gaacaatttg tattattatt ctcatactac tacctcccga aagtttactg
     3781 aatttgctat gaccatataa acctcccctg taaaagtccc agatacaaaa atgatcaatt
     3841 cccgacagga ttttgttttg gtgtgaatta atttcactag aaatatacca aaaactaata
     3901 aaaccccaaa ttttctactc aataatcaat taatatctaa aactcaaacc aagactttaa
     3961 tgccggatgg gtatttttat ggcaaaccct ttattattac atcacgcacg ctcaatggta
     4021 gacaaccttt ttatactatc tgttatgcaa atggtaaatg tgaaaatgtt acagaagaca
     4081 caattagaca tcatgctcca caagcagtca gaaaatatag gttacggaat tgagagctga
     4141 caagttacta agtgaagaag ttgatttact ttttgtgcag ttgttttgga tgaccttttt
     4201 tgagttatat gctagtatat tatagtgtat gtgacatttg atcttgataa taagctagta
     4261 gtcaaagcaa agagaaattt gggacataat tgtgatcaaa tagaattatc acgacaactt
     4321 gtttaaatgc aaaggggatt aaatactttt agatggaagc ttttagtttg agtactttag
     4381 atgattaaag ataagcgtaa tacttttgat atctctatat gctaccacta cacgatccca
     4441 aaatgttgtg atatttcaat cgccatttaa atcttgttca ttaacaccat gtggtatcac
     4501 aataacaaat gactcaatgt gtaatttaaa ttataaattg ataatcctat taatcacata
     4561 tgaaagctca accttggtta aagcaaccac aacctataaa taatgcctgg attgccgcag
     4621 ttacaaatgc atgttatttc tttacattat ttcaaattcc ccaggaacga aacccctaca
     4681 accccctgac ttttttttgt caaaactaga gtaatagcct acaatgtctg atggatacat
     4741 gaatggtatg cggtttaata ttgctacgtg tactactcga tcaggagtgt tttactatgt
     4801 catttgttac gaaaatggga tgcaggaaac tatgagtgaa gcgaagctta gatattatga
     4861 tccccagtca attagtaaat tcaataaacg aattaataaa aagtgacaaa ctggcgatag
     4921 tgttagtatt tttcctttgt ctttcaattg agaagattta gttaccaact acaagagtat
     4981 attgtttata tttattttta ggtgatatat ttcaatttta cattaaaatt tctattaaaa
     5041 ataacctttt cttgaagtga atttaaatga ctatacaaga tcatagagct atgtagacaa
     5101 actctgtaat cgttcaaagt ttgatttgag atttagtttt tttctttttt agtttaaaag
     5161 tttaaaacac aaaggcaaag gatgaaatag tgattttttt gcaacttcct tattaacaat
     5221 tatttaatgt aaattgcaac acgaaaggat tgtataaatc tatcactggt tgtagaactg
     5281 tcccatatag cttgaaaact ttagctataa caaaataaat acaacaattt gaaattctta
     5341 gttatagaat ttgattatag tttcataatt caatttctga tattattggt ggtgaaaagg
     5401 ggctgaaatt attgatcagg aatgtaattt taaggacaaa agttttctgc attggaaaat
     5461 accgcaatta actctaaaca atgtgacgca ttttgcagaa gtttaaagga tatcgaaatt
     5521 cttgtcatta ctataaatac ctacgccaat cacccagggg gtaaaaaaaa aagtaacttc
     5581 ttgcttcatg agattatgta aatagtttgt tttttgacat cagcaacaat tagccaagct
     5641 aaaacaaaca cacttaacac aatcgtcaac tacaattaat caaaatgcca gacggaatct
     5701 acaataacga accctttact attatcaaac gtgaaatcat gggcggagta ccttgcgcgc
     5761 gctacggatc tcgagcgaag tagtcctcac tgaggagatc ccaggctaga aatcagcaaa
     5821 ttttacatcc ctcatattct gacacgagtt taactacata gcaattgtaa aagggctctt
     5881 gttttttttt tttttttgtg gcggtgcagt acgtacacga tctttaaaga ttagggctat
     5941 aaccatgtca aagtgactca ttttttttgt tcaaatgcgc actcggattt aaagcagcaa
     6001 aaaaatactt cgttacaaaa atggaagcgc attttccgaa aaaggttaat tcctagcgat
     6061 tttattattt ttgttttatt tattaatttt tcttgttttg ctattgcatt tttgcaacca
     6121 aaaatagaaa attctcgttt ttttttgttg tagcgcaacc aatttctggt tattcttttt
     6181 tttttgcaat ttttaaattt gcacgtgaca gtgttagtgt tagtgcaggc ctgatatccc
     6241 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     6301 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     6361 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     6421 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     6481 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     6541 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     6601 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     6661 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     6721 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     6781 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     6841 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     6901 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     6961 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     7021 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     7081 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     7141 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     7201 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     7261 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     7321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     7381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     7441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     7501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     7561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     7621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     7681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7981 gccttttgct cacatgtt
//
