LOCUS       V016270                 8333 bp    DNA     circular SYN 20-MAR-2021
DEFINITION  Exported.
ACCESSION   V016270
VERSION     V016270
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Nemeth TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (27-AUG-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..8333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    570..575
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="Site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     misc_feature    1040..1047
                     /label="NotI site for selection marker ligation"
                     /note="NotI site for selection marker ligation"
     regulatory      1096..1943
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     CDS             1944..3062
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label="Candida maltosa LEU2 terminator"
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     misc_feature    3314..3319
                     /note="BssHII site for selection marker and insert
                     ligation"
     misc_feature    3320..6091
                     /note="Candida albicans intergenic region;
                     Ca22chrRB_C_albicans_SC5314:2080135-2082906"
     misc_feature    6092..6097
                     /note="BssHII site for selection marker and insert
                     ligation"
     misc_feature    6105..6110
                     /label="XhoI site for insert ligation"
                     /note="XhoI site for insert ligation"
     misc_feature    6111..6561
                     /note="Site of recombination; CpNEUT5LDowndown from C.
                     parapsilosis"
     misc_feature    6562..6567
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     promoter        complement(6575..6593)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6598..6614)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             6727..7533
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7683..8271
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcg cccttggatc caatcatcac tggtgggggt caagaaagag gtttgagatc
     1141 aggtactttg gcaccaccat tagtagctgg gtttggtgaa gctgcaagat taatgaaaca
     1201 agaatctgca tttgacaaga aacatattga gaggttatct actaaattga agaacggttt
     1261 gttatccatt ccatctactc agtttaacgg ttgtaataac ccaacatatc aatacccagg
     1321 ttgtgtgaat gtttcctttg catacattga aggggaatct ttgttaatgg cattgaaaga
     1381 tattgcattg agttctggtt ctgcatgtac ctctgcatct ttggaaccat cgtatgtttt
     1441 acatgctttg ggtgccgatg atgccttggc tcattcttcc attagattcg gtattggtag
     1501 attcaccact gaagcagaag ttgactatgt tattcaagca attaatgaaa gagttgattt
     1561 cttgagaaag atgtctccat tatgggaaat ggttcaagaa ggtattgact tgaactctat
     1621 cgaatggagt ggacattaag gttttttgtt tttttgtaat ttgtgttgtt atgtgtaatt
     1681 atcgtgtata gagtaatttc ggaaattgta ctttcgttca tgtatctgaa ccagatgaat
     1741 aatacgttcg tattgttgaa gaatgactaa ctcctgtaac ggcgtgcgtg ggttccgcag
     1801 ccgggttcaa tttttttttt cttgttcact ataaaaatat ataataaacg aaaaaaatcc
     1861 gagggactca ggctatgaaa tttttcactc aagaattttt tttccttcat ccttaattcc
     1921 tttattcact acccaaaccc acaatgtcag ttaaaactaa aactattaca attttaccag
     1981 gtgatcacgt cggtaccgaa attgtcaatg aagctatcaa agttttagaa gcaattgaag
     2041 ctgccactcc ttaccaaaaa atccatttcg atttcaaaca tcatttaatc ggtggtgctg
     2101 ccattgatgc cactggtgtc ccattgccag atgacgcact tgaaagtgcc aaaaaatctg
     2161 atgccgtctt attgggtgct gttggtggac caaaatgggg gactggcgcc gttcgtccag
     2221 aacaaggttt attaaagatt cgtaaagaat tgaaccttta tgctaacatc agaccatgta
     2281 actttgccag tgactcctta ttggaattat ccccattgag accagaagtc gttaaaggta
     2341 caaacttgat cattgttcgt gaattagttg gtggtattta tttcggtgat cgtgaagaac
     2401 aagaagaaag tgaagataaa caaaccgcat gggatactga aaaatacacc gtcgatgaag
     2461 tcaccagaat cacccgtatg gctgccttca tggctttaca acataaccca ccattaccaa
     2521 tctggtcatt ggataaggct aatgttttag cttcttctag attatggaga agaactgtcg
     2581 ataaagtgat ttctgaagag ttcccaactt tgtctgttca acatcaattg attgactccg
     2641 ccgccatgat tttaattcaa aacccaacca aattgaatgg tataatcatc acttctaaca
     2701 tgtttggtga tatcatttcc gatgaagctt cagttattcc aggttccttg ggtttattac
     2761 catctgcatc tttggcttca ttgccagata ccaacaccgc ttttggtctt tatgaaccat
     2821 gtcacggttc tgctcctgat ttaccagcca ataaagttaa cccaattgct actattttat
     2881 ctgctgcttc tatgttgaga ttatctttgg attgtgtgaa agaagctgaa gctttagaag
     2941 aagccgtgaa acaagtcttg gataaaggta tcagaactgc agatttaaga ggtaccagtt
     3001 cagctactga agttggtgac gcgattgtcg aagctgttac taaaatctta aaagaaaaag
     3061 cttaagtaaa gtatacacaa aatttaacga atattattaa tacaaaatga acaccaccta
     3121 aatacttttt tttaaaaatg taccgactct tctaaaaatt gcgataattc aatatcggaa
     3181 atttctctca ccggtttact tggatcttcg gaaataaatc tatttgaagt aatggtagcc
     3241 tcacttgatt ctgattggct ctttctagac atgggtaggt aagggcgaat tctgcagata
     3301 tccatcacac tgggcgcgct gcaactttgt tctaaatggt tcgattggat tctcccaatt
     3361 acacaaaaat tattattttc gtgctgtttt ggcgccgact tctatttttt aaatcccaac
     3421 agggattccg atccaaatca gcaggtattt ctatctgatt tcttttcaat ttggttgctt
     3481 ttttctgaac ttatttcgta ttaatgggca acaatttata aagattattc agaatactag
     3541 ttgaaaaaat tgtgtatagt aagcttcata tggaattaaa ttgaatgatt ttagttatgt
     3601 atttttaagg agtttctttc catactatgg gatatggaaa tatgtatatt ctatttcata
     3661 tcccttttct tgtttccaat ttgaatattc aatttccgaa aaacaaaaaa gaacacaaaa
     3721 caaaacaact aataatgatg atgttttcat tctgttttgt gtaatattcc ttctcctgac
     3781 taataaattg atatatatgg cataatacaa aaatgagtga agctaaattc taattgcatg
     3841 ttgcaattta ttctagcctt taaaaatgtc ggaattgaga atgtgttgta atgttgtatt
     3901 gtatccctta aactactaca ccattacagt ttttttttgt gttattgttt ttgaaaaaag
     3961 tcaaaattag aaagttacag ggatagtttc aatagtttct ggtaggttcc tcctaaagtt
     4021 gaatttgggt agaaagacag ccaggatcct tggattgttt agtatgaaca atttgtatta
     4081 ttattctcat actactacct cccgaaagtt tactgaattt gctatgacca tataaacctc
     4141 ccctgtaaaa gtcccagata caaaaatgat caattcccga caggattttg ttttggtgtg
     4201 aattaatttc actagaaata taccaaaaac taataaaacc ccaaattttc tactcaataa
     4261 tcaattaata tctaaaactc aaaccaagac tttaatgccg gatgggtatt tttatggcaa
     4321 accctttatt attacatcac gcacgctcaa tggtagacaa cctttttata ctatctgtta
     4381 tgcaaatggt aaatgtgaaa atgttacaga agacacaatt agacatcatg ctccacaagc
     4441 agtcagaaaa tataggttac ggaattgaga gctgacaagt tactaagtga agaagttgat
     4501 ttactttttg tgcagttgtt ttggatgacc ttttttgagt tatatgctag tatattatag
     4561 tgtatgtgac atttgatctt gataataagc tagtagtcaa agcaaagaga aatttgggac
     4621 ataattgtga tcaaatagaa ttatcacgac aacttgttta aatgcaaagg ggattaaata
     4681 cttttagatg gaagctttta gtttgagtac tttagatgat taaagataag cgtaatactt
     4741 ttgatatctc tatatgctac cactacacga tcccaaaatg ttgtgatatt tcaatcgcca
     4801 tttaaatctt gttcattaac accatgtggt atcacaataa caaatgactc aatgtgtaat
     4861 ttaaattata aattgataat cctattaatc acatatgaaa gctcaacctt ggttaaagca
     4921 accacaacct ataaataatg cctggattgc cgcagttaca aatgcatgtt atttctttac
     4981 attatttcaa attccccagg aacgaaaccc ctacaacccc ctgacttttt tttgtcaaaa
     5041 ctagagtaat agcctacaat gtctgatgga tacatgaatg gtatgcggtt taatattgct
     5101 acgtgtacta ctcgatcagg agtgttttac tatgtcattt gttacgaaaa tgggatgcag
     5161 gaaactatga gtgaagcgaa gcttagatat tatgatcccc agtcaattag taaattcaat
     5221 aaacgaatta ataaaaagtg acaaactggc gatagtgtta gtatttttcc tttgtctttc
     5281 aattgagaag atttagttac caactacaag agtatattgt ttatatttat ttttaggtga
     5341 tatatttcaa ttttacatta aaatttctat taaaaataac cttttcttga agtgaattta
     5401 aatgactata caagatcata gagctatgta gacaaactct gtaatcgttc aaagtttgat
     5461 ttgagattta gtttttttct tttttagttt aaaagtttaa aacacaaagg caaaggatga
     5521 aatagtgatt tttttgcaac ttccttatta acaattattt aatgtaaatt gcaacacgaa
     5581 aggattgtat aaatctatca ctggttgtag aactgtccca tatagcttga aaactttagc
     5641 tataacaaaa taaatacaac aatttgaaat tcttagttat agaatttgat tatagtttca
     5701 taattcaatt tctgatatta ttggtggtga aaaggggctg aaattattga tcaggaatgt
     5761 aattttaagg acaaaagttt tctgcattgg aaaataccgc aattaactct aaacaatgtg
     5821 acgcattttg cagaagttta aaggatatcg aaattcttgt cattactata aatacctacg
     5881 ccaatcaccc agggggtaaa aaaaaaagta acttcttgct tcatgagatt atgtaaatag
     5941 tttgtttttt gacatcagca acaattagcc aagctaaaac aaacacactt aacacaatcg
     6001 tcaactacaa ttaatcaaaa tgccagacgg aatctacaat aacgaaccct ttactattat
     6061 caaacgtgaa atcatgggcg gagtaccttg cgcgcgctac ggatctcgag cgaagtagtc
     6121 ctcactgagg agatcccagg ctagaaatca gcaaatttta catccctcat attctgacac
     6181 gagtttaact acatagcaat tgtaaaaggg ctcttgtttt tttttttttt ttgtggcggt
     6241 gcagtacgta cacgatcttt aaagattagg gctataacca tgtcaaagtg actcattttt
     6301 tttgttcaaa tgcgcactcg gatttaaagc agcaaaaaaa tacttcgtta caaaaatgga
     6361 agcgcatttt ccgaaaaagg ttaattccta gcgattttat tatttttgtt ttatttatta
     6421 atttttcttg ttttgctatt gcatttttgc aaccaaaaat agaaaattct cgtttttttt
     6481 tgttgtagcg caaccaattt ctggttattc tttttttttt gcaattttta aatttgcacg
     6541 tgacagtgtt agtgttagtg caggcctgat atcccctata gtgagtcgta ttacatggtc
     6601 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     6661 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     6721 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     6781 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     6841 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     6901 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     6961 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     7021 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     7081 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     7141 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     7201 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     7261 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     7321 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     7381 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     7441 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     7501 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     7561 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     7621 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
