LOCUS       V004281                 5824 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004281
VERSION     V004281
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Charpentier E, Anton AI, Barry P, Alfonso B, Fang Y, Novick RP.
  TITLE     Novel cassette-based shuttle vector system for gram-positive
            bacteria
  JOURNAL   Appl. Environ. Microbiol. 70 (10), 6076-6085 (2004)
   PUBMED   15466553
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Riojas MA, Bojja K, Farley T, Moreno J, Pederson C, Byrd R, Hamid S,
            Mooney S, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) BEI Bacteriology, BEI Resources/American
            Type and Culture Collection (ATCC), 10801 University Boulevard,
            Manassas, VA 20110-2209, USA
REFERENCE   3  (bases 1 to 5824)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Coverage              :: 11,677.82
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2004"; volume: "70"; issue: "10";
            pages: "6076-6085"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2014) BEI Bacteriology, BEI Resources/American Type and
            Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA
            20110-2209, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1316..1332
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1333..1377
                     /note="HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-
                     EcoRI; polylinker of M13mp19"
     regulatory      complement(1378..1511)
                     /note="constitutive beta-lactamase promoter; P_blaZ"
                     /regulatory_class="promoter"
     rep_origin      complement(1648..2236)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2410..3267)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3268..3372)
                     /label="AmpR promoter"
     CDS             complement(3740..4471)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             complement(4899..5204)
                     /codon_start=1
                     /product="truncated polypeptide B"
                     /label="truncated polypeptide B"
                     /protein_id="AJF23042.1"
                     /translation="MFSLYKKFKGLFYSVLFWLCILSFFSVLNEMVLNVSLPDIANHFN
                     TTPGITNWVNTAYMLTFSIGTAVYGKLSDYINIKKLLIIGISLSCLGSLIAFIGPT"
ORIGIN
        1 aactgcttta tcagacattg catagtagtc actcaaatca tcttcaaaat caaaggctaa
       61 atctaatctt gtaaaaccgt catcttccat gtagcttatt atattttgtt ttaaccaaat
      121 catttcatct cgtgtaagtt tgtttggatt aaattcaata cgcatattac gtctatccca
      181 actatctgct ttcactttgt catattcaat ataaactttt tcttgaagtg ctttagcttt
      241 aaactttgtt tgaagaatat cccaaagtct tatttggggc tctacactca taaatttaga
      301 aagggcttga gcgttgtctc ggttgagatt tccaacgatc gtcattgcat caaaacttaa
      361 ttttggatca gatatgcaca cagtatgtgc gtccaaccgg ctattagagt agccggtttt
      421 agaaaaattg tctaaatcgt gattttccaa atgatttgaa tgatttgcat gattgttttt
      481 atacataaaa aatcgactcc ttaatctcaa tttcgtttaa ggaatcgctc acccaaatat
      541 atatcttgat gtatatttaa atatcgttta atatctaaat atacaagatt ataaaaacaa
      601 ctcagtgttt ttttctttga atgatgtcgt tcacaaactt tggtcagggc gtgagcgact
      661 cctttttatt ttgttattaa tataacacta tcaaaagatt tggtctaatc agatcaagtc
      721 tttttttatt taagcatttg tattatctgg taaacagtta aaactactaa aacaccaagt
      781 acatacttac ttgttataaa attctcaaag gcttctctga atttcatttc ttcaactcct
      841 tttattgctt tattccaatt tccttattgg tcggaaccta cagggcttta tgtgttgttg
      901 agttggtact ttcttgggat taatcccaat tcaagtccaa ccaactcgct aacaagttag
      961 ctaacacata gcccattcca accaataagt tttctcggca taaatgcatg ttctataagt
     1021 gcatacctct atctcgtttt tgattctttt gttgttcttg ttgctgttcc tgttctgaat
     1081 ataattctgg attcattttt ttatctaagt ttcctgctaa accatttagt tttggttcat
     1141 cttctgttaa cttattaact ctttccgcaa aggcgccatt cgccattcag gctgcgcaac
     1201 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     1261 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     1321 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcagaat
     1381 aaaccctccg atattacagt tgtaattttt ataaatcaat aatattacaa ttgtaatatc
     1441 ggagggttta ttataaatat ttgaaagaag gtgttgaaat ggctaagtta ttaataatgg
     1501 catagtaagc tgcatgcagc ggccgccagc tcactcaaag gcggtaatac ggttatccac
     1561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3361 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3421 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     3481 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3541 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3601 gggctggctt aactatgcgg ccgctcgagc gccgcggaaa actggtttaa gccgactgcg
     3661 caaaagacat aatcgattca caaaaaatag gcacacgaaa aacaagttaa gggatgcagt
     3721 ttatgcatcc cttaacttac ttattaaata atttatagct attgaaaaga gataagaatt
     3781 gttcaaagct aatattgttt aaatcgtcaa ttcctgcatg ttttaaggaa ttgttaaatt
     3841 gattttttgt aaatattttc ttgtattctt tgttaaccca tttcataacg aaataattat
     3901 acttttgttt atctttgtgt gatattcttg atttttttct acttaatctg ataagtgagc
     3961 tattcacttt aggtttagga tgaaaatatt ctcttggaac catacttaat atagaaatat
     4021 caacttctgc cattaaaagt aatgccaatg agcgttttgt atttaataat cttttagcaa
     4081 acccgtattc cacgattaaa taaatctcat tagctatact atcaaaaaca attttgcgta
     4141 ttatatccgt acttatgtta taaggtatat taccatatat tttataggat tggtttttag
     4201 gaaatttaaa ctgcaatata tccttgttta aaacttggaa attatcgtga tcaacaagtt
     4261 tattttctgt agttttgcat aatttatggt ctatttcaat ggcagttacg aaattacacc
     4321 tctttactaa ttcaagggta aaatggcctt ttcctgagcc gatttcaaag atattatcat
     4381 gttcatttaa tcttatattt gtcattattt tatctatatt atgttttgaa gtaataaagt
     4441 tttgactgtg ttttatattt ttctcgttca ttataaccct ctttaatttg gttatatgaa
     4501 ttttgcttat taacgattca ttataaccac ttattttttg tttggttgat aatgaactgt
     4561 gctgattaca aaaatactaa aaatgcccat attttttcct ccttataaaa ttagtataat
     4621 tatagcacga gctctgataa atatgaacat gatgagtgat cgttaaattt atactgcaat
     4681 cggatgcgat tattgaataa aagatatgag agatttatct aatttctttt ttcttgtaaa
     4741 aaaagaaagt tcttaaaggt tttatagttt tggtcgtaga gcacacggtt taacgactta
     4801 attacgaagt aaataagtct agtgtgttag actttatgaa atctatatac gtttatatat
     4861 atttattatc cggaggtgta gcatgtctca ttcaattcct aggtgggccc aataaaagca
     4921 atcaatgaac caagacagct caaactaata ccaataatta acaatttttt tatatttata
     4981 taatcagata attttccata tactgctgtt cctatcgaaa aagttaacat atatgcagtg
     5041 tttacccagt ttgtaattcc aggagtagta ttaaaatgat ttgcaatatc aggtaaagaa
     5101 acatttaaaa ccatttcatt taatacacta aaaaatgaaa gaatacaaag ccaaaataaa
     5161 acgctataaa acaaaccttt aaatttttta tataaactaa acaattttac ctctttactt
     5221 gaggtgacta aagtttatag gggtaaatta ttctttcacc tcatataaat tccccaaatt
     5281 tttaaataga cacttcataa aaaaatcctc ctaaattaga atagtttaaa tatatcattt
     5341 ttgttcagta atattaatat gtacactatt tccaaaattt aaattcatgt tgccaaaaat
     5401 cgatttgttt ttgcaattgt ttttcgttcg cttttaaagt cgatttcatt aagtccgtta
     5461 aatcgactgg cgaaatttct tttatcaaat tcttatattt tgttctagaa tttctgtgaa
     5521 gctttcccca ttcttcttca tcactcaata acataaaaac tattgctcta tccgcagtgc
     5581 gttggatagt tttccaatct ggttgcaaga tatgtaaatc actaaagcaa tcattccagt
     5641 aatccaccat atctctttta agttcgattt ctacacgcca taaatgttca gacataactt
     5701 cagcatctgc attatcttta cgttcttgct ttttattata aattctaata aatctattac
     5761 tatctctcac gccaaaatat tttgtttctg gcttaccatt acgaccataa aaaatagttt
     5821 tctt
//
