LOCUS       40924_33477        5544 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNR-46121, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Charpentier E, Anton AI, Barry P, Alfonso B, Fang Y, Novick RP.
  TITLE     Novel cassette-based shuttle vector system for gram-positive 
            bacteria
  JOURNAL   Appl. Environ. Microbiol. 70 (10), 6076-6085 (2004)
  PUBMED    15466553
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Riojas MA, Bojja K, Hazbon MH.
  TITLE     pNR-46121, A Shuttle Vector for Gram-Positive Bacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5544)
  AUTHORS   Riojas MA, Bojja K, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Bacteriology, BEI Resources/American Type 
            Culture Collection (ATCC), 10801 University Blvd., Manassas, VA 
            20110, USA
REFERENCE   4  (bases 1 to 5544)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2004"; volume: "70"; issue: "10"; 
            pages: "6076-6085"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Bacteriology, BEI Resources/American Type Culture 
            Collection (ATCC), 10801 University Blvd., Manassas, VA 20110, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1417
                     /note="pT181 cop-wt repC; derived from J01764.1
                     Staphylococcus aureus plasmid pT181"
     CDS             157..960
                     /codon_start=1
                     /gene="repC"
                     /product="replication initiation protein"
                     /label=repC
                     /protein_id="AIL56502.1"
                     /translation="MTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVY
                     IEYDKVKADSWDRRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDL
                     SDYYAMSDKAVKKTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHL
                     WRVEIELKRDMVDYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSR
                     TKYKNLIKEISPVDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     gene            157..960
                     /gene="repC"
                     /label=repC
     misc_feature    1122..1417
                     /gene="tetK"
                     /label=truncated tetracycline efflux protein
                     /note="truncated tetracycline efflux protein"
     gene            1122..1417
                     /gene="tetK"
                     /label=tetK
     CDS             1611..2402
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        2808..2912
                     /label=AmpR promoter
     CDS             2913..3770
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3944..4532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4714..4730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4873..5544
                     /note="pT181 cop-wt repC; derived from J01764.1
                     Staphylococcus aureus plasmid pT181"
ORIGIN
        1 aggagtcgat tttttatgta taaaaacaat catgcaaatc attcaaatca tttggaaaat
       61 cacgatttag acaatttttc taaaaccggc tactctaata gccggttgga cgcacatact
      121 gtgtgcatat ctgatccaaa attaagtttt gatgcaatga cgatcgttgg aaatctcaac
      181 cgagacaacg ctcaagccct ttctaaattt atgagtgtag agccccaaat aagactttgg
      241 gatattcttc aaacaaagtt taaagctaaa gcacttcaag aaaaagttta tattgaatat
      301 gacaaagtga aagcagatag ttgggataga cgtaatatgc gtattgaatt taatccaaac
      361 aaacttacac gagatgaaat gatttggtta aaacaaaata taataagcta catggaagat
      421 gacggtttta caagattaga tttagccttt gattttgaag atgatttgag tgactactat
      481 gcaatgtctg ataaagcagt taagaaaact attttttatg gtcgtaatgg taagccagaa
      541 acaaaatatt ttggcgtgag agatagtaat agatttatta gaatttataa taaaaagcaa
      601 gaacgtaaag ataatgcaga tgctgaagtt atgtctgaac atttatggcg tgtagaaatc
      661 gaacttaaaa gagatatggt ggattactgg aatgattgct ttagtgattt acatatcttg
      721 caaccagatt ggaaaactat ccaacgcact gcggatagag caatagtttt tatgttattg
      781 agtgatgaag aagaatgggg aaagcttcac agaaattcta gaacaaaata taagaatttg
      841 ataaaagaaa tttcgccagt cgatttaacg gacttaatga aatcgacttt aaaagcgaac
      901 gaaaaacaat tgcaaaaaca aatcgatttt tggcaacatg aatttaaatt ttggaaatag
      961 tgtacatatt aatattactg aacaaaaatg atatatttaa actattctaa tttaggagga
     1021 tttttttatg aagtgtctat ttaaaaattt ggggaattta tatgaggtga aagaataatt
     1081 tacccctata aactttagtc acctcaagta aagaggtaaa attgtttagt ttatataaaa
     1141 aatttaaagg tttgttttat agcgttttat tttggctttg tattctttca ttttttagtg
     1201 tattaaatga aatggtttta aatgtttctt tacctgatat tgcaaatcat tttaatacta
     1261 ctcctggaat tacaaactgg gtaaacactg catatatgtt aactttttcg ataggaacag
     1321 cagtatatgg aaaattatct gattatataa atataaaaaa attgttaatt attggtatta
     1381 gtttgagctg tcttggttca ttgattgctt ttattgggcc cacctagggg tttcaaaatc
     1441 ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc
     1501 tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta
     1561 gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa
     1621 tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg
     1681 aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa
     1741 aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga
     1801 tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg
     1861 gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag
     1921 atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact
     1981 ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg
     2041 attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc
     2101 catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac
     2161 ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa
     2221 gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct
     2281 gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact
     2341 tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt
     2401 tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta gacatctaat
     2461 cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt ttctaaaagt
     2521 tcgctagata ggggtcccga gcgccgcggc ctcgagcggc cgcatagtta agccagcccc
     2581 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2641 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2701 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2761 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2821 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2881 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2941 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3001 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3061 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3121 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3181 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3241 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3301 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3361 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3421 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3481 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3541 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3601 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3661 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3721 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3781 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4081 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4561 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4621 tggataaccg tattaccgcc tttgagtgag ctggcggccg ctgcatgcct gcaggtcgac
     4681 tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
     4741 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4801 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4861 tggcgaatgg cgcctttgcg gaaagagtta ataagttaac agaagatgaa ccaaaactaa
     4921 atggtttagc aggaaactta gataaaaaaa tgaatccaga attatattca gaacaggaac
     4981 agcaacaaga acaacaaaag aatcaaaaac gagatagagg tatgcactta tagaacatgc
     5041 atttatgccg agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga
     5101 gttggttgga cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag
     5161 ccctgtaggt tccgaccaat aaggaaattg gaataaagca ataaaaggag ttgaagaaat
     5221 gaaattcaga gaagcctttg agaattttat aacaagtaag tatgtacttg gtgttttagt
     5281 agttttaact gtttaccaga taatacaaat gcttaaataa aaaaagactt gatctgatta
     5341 gaccaaatct tttgatagtg ttatattaat aacaaaataa aaaggagtcg ctcacgccct
     5401 gaccaaagtt tgtgaacgac atcattcaaa gaaaaaaaca ctgagttgtt tttataatct
     5461 tgtatattta gatattaaac gatatttaaa tatacatcaa gatatatatt tgggtgagcg
     5521 attccttaaa cgaaattgag atta
//
