LOCUS       40924_33457       13292 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV141, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13292)
  AUTHORS   Dean C, Barkan D, Bermingham A, Blais J, Casey F, Casarez A, Colvin 
            R, Fuller J, Jones A, Li C, Lopez S, Metzger L, Prathapam R, Rasper 
            D, Reck F, Ruzin A, Shaul J, Shen X, Simmons R, Skewes-Cox P, 
            Tamrakar P, Uehara T.
  TITLE     The monobactam LYS228: mode of action and mechanisms decreasing in 
            vitro susceptibility of Escherichia coli and Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13292)
  AUTHORS   Wei J-R., Mostafavi M, Takeoka K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2018) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 13292)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13292)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2018) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(44..1123)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1124..1201)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1431..1459
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1467..1483
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1506..5609
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5749..6624)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        6651..6935
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             6962..6970
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6979..7392
                     /codon_start=1
                     /label=Gam
                     /note="inhibitor of the host RecBCD nuclease in the lambda 
                     Red system"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             7401..8183
                     /codon_start=1
                     /label=Beta
                     /note="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             8183..8860
                     /codon_start=1
                     /label=Exo
                     /note="5' to 3' double-stranded DNA exonuclease in the 
                     lambda
                     Red system"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      8864..9108
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     rep_origin      complement(9754..10299)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(10617..12035)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      12272..12299
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        12318..12409
                     /label=AmpR promoter
     CDS             12410..13267
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctcgagcaat tcgcgctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
       61 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      121 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
      181 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
      241 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
      301 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
      361 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
      421 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
      481 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
      541 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
      601 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
      661 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
      721 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
      781 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
      841 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
      901 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
      961 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     1021 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     1081 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     1141 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcaccat tcgatggtgt
     1201 caacgtaaat gccgcttcgc cttcgcgcgc gaattgcaag ctgatccggg cttatcgact
     1261 gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc
     1321 aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt
     1381 tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta
     1441 atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
     1501 aattcatgga taagaaatac tcaataggct tagatatcgg cacaaatagc gtcggatggg
     1561 cggtgatcac tgatgaatat aaggttccgt ctaaaaagtt caaggttctg ggaaatacag
     1621 accgccacag tatcaaaaaa aatcttatag gggctctttt atttgacagt ggagagacag
     1681 cggaagcgac tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg aagaatcgta
     1741 tttgttatct acaggagatt ttttcaaatg agatggcgaa agtagatgat agtttctttc
     1801 atcgacttga agagtctttt ttggtggaag aagacaagaa gcatgaacgt catcctattt
     1861 ttggaaatat agtagatgaa gttgcttatc atgagaaata tccaactatc tatcatctgc
     1921 gaaaaaaatt ggtagattct actgataaag cggatttgcg cttaatctat ttggccttag
     1981 cgcatatgat taagtttcgt ggtcattttt tgattgaggg agatttaaat cctgataata
     2041 gtgatgtgga caaactattt atccagttgg tacaaaccta caatcaatta tttgaagaaa
     2101 accctattaa cgcaagtgga gtagatgcta aagcgattct ttctgcacga ttgagtaaat
     2161 caagacgatt agaaaatctc attgctcagc tccccggtga gaagaaaaat ggcttatttg
     2221 ggaatctcat tgctttgtca ttgggtttga cccctaattt taaatcaaat tttgatttgg
     2281 cagaagatgc taaattacag ctttcaaaag atacttacga tgatgattta gataatttat
     2341 tggcgcaaat tggagatcaa tatgctgatt tgtttttggc agctaagaat ttatcagatg
     2401 ctattttact ttcagatatc ctaagagtaa atactgaaat aactaaggct cccctatcag
     2461 cttcaatgat taaacgctac gatgaacatc atcaagactt gactctttta aaagctttag
     2521 ttcgacaaca acttccagaa aagtataaag aaatcttttt tgatcaatca aaaaacggat
     2581 atgcaggtta tattgatggg ggagctagcc aagaagaatt ttataaattt atcaaaccaa
     2641 ttttagaaaa aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc
     2701 tgcgcaagca acggaccttt gacaacggct ctattcccca tcaaattcac ttgggtgagc
     2761 tgcatgctat tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga
     2821 agattgaaaa aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca
     2881 atagtcgttt tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg
     2941 aagaagttgt cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg
     3001 ataaaaatct tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta
     3061 cggtttataa cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat
     3121 ttctttcagg tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag
     3181 taaccgttaa gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt gatagtgttg
     3241 aaatttcagg agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa
     3301 aaattattaa agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata
     3361 ttgttttaac attgacctta tttgaagata gggagatgat tgaggaaaga cttaaaacat
     3421 atgctcacct ctttgatgat aaggtgatga aacagcttaa acgtcgccgt tatactggtt
     3481 ggggacgttt gtctcgaaaa ttgattaatg gtattaggga taagcaatct ggcaaaacaa
     3541 tattagattt tttgaaatca gatggttttg ccaatcgcaa ttttatgcag ctgatccatg
     3601 atgatagttt gacatttaaa gaagacattc aaaaagcaca agtgtctgga caaggcgata
     3661 gtttacatga acatattgca aatttagctg gtagccctgc tattaaaaaa ggtattttac
     3721 agactgtaaa agttgttgat gaattggtca aagtaatggg gcggcataag ccagaaaata
     3781 tcgttattga aatggcacgt gaaaatcaga caactcaaaa gggccagaaa aattcgcgag
     3841 agcgtatgaa acgaatcgaa gaaggtatca aagaattagg aagtcagatt cttaaagagc
     3901 atcctgttga aaatactcaa ttgcaaaatg aaaagctcta tctctattat ctccaaaatg
     3961 gaagagacat gtatgtggac caagaattag atattaatcg tttaagtgat tatgatgtcg
     4021 atcacattgt tccacaaagt ttccttaaag acgattcaat agacaataag gtcttaacgc
     4081 gttctgataa aaatcgtggt aaatcggata acgttccaag tgaagaagta gtcaaaaaga
     4141 tgaaaaacta ttggagacaa cttctaaacg ccaagttaat cactcaacgt aagtttgata
     4201 atttaacgaa agctgaacgt ggaggtttga gtgaacttga taaagctggt tttatcaaac
     4261 gccaattggt tgaaactcgc caaatcacta agcatgtggc acaaattttg gatagtcgca
     4321 tgaatactaa atacgatgaa aatgataaac ttattcgaga ggttaaagtg attaccttaa
     4381 aatctaaatt agtttctgac ttccgaaaag atttccaatt ctataaagta cgtgagatta
     4441 acaattacca tcatgcccat gatgcgtatc taaatgccgt cgttggaact gctttgatta
     4501 agaaatatcc aaaacttgaa tcggagtttg tctatggtga ttataaagtt tatgatgttc
     4561 gtaaaatgat tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt
     4621 actctaatat catgaacttc ttcaaaacag aaattacact tgcaaatgga gagattcgca
     4681 aacgccctct aatcgaaact aatggggaaa ctggagaaat tgtctgggat aaagggcgag
     4741 attttgccac agtgcgcaaa gtattgtcca tgccccaagt caatattgtc aagaaaacag
     4801 aagtacagac aggcggattc tccaaggagt caattttacc aaaaagaaat tcggacaagc
     4861 ttattgctcg taaaaaagac tgggatccaa aaaaatatgg tggttttgat agtccaacgg
     4921 tagcttattc agtcctagtg gttgctaagg tggaaaaagg gaaatcgaag aagttaaaat
     4981 ccgttaaaga gttactaggg atcacaatta tggaaagaag ttcctttgaa aaaaatccga
     5041 ttgacttttt agaagctaaa ggatataagg aagttaaaaa agacttaatc attaaactac
     5101 ctaaatatag tctttttgag ttagaaaacg gtcgtaaacg gatgctggct agtgccggag
     5161 aattacaaaa aggaaatgag ctggctctgc caagcaaata tgtgaatttt ttatatttag
     5221 ctagtcatta tgaaaagttg aagggtagtc cagaagataa cgaacaaaaa caattgtttg
     5281 tggagcagca taagcattat ttagatgaga ttattgagca aatcagtgaa ttttctaagc
     5341 gtgttatttt agcagatgcc aatttagata aagttcttag tgcatataac aaacatagag
     5401 acaaaccaat acgtgaacaa gcagaaaata ttattcattt atttacgttg acgaatcttg
     5461 gagctcccgc tgcttttaaa tattttgata caacaattga tcgtaaacga tatacgtcta
     5521 caaaagaagt tttagatgcc actcttatcc atcaatccat cactggtctt tatgaaacac
     5581 gcattgattt gagtcagcta ggaggtgact gacccgggtg ttgcatgggc ataaagttgc
     5641 ctttttaatc acaattcaga aaatatcata atatctcatt tcactaaata atagtgaacg
     5701 gcaggtatat gtgatgggtt aaaaaggatc gatcctctag atttattatg acaacttgac
     5761 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt
     5821 gcatttttta aatacccgcg agaagtagag ttgatcgtca aaaccaacat tgcgaccgac
     5881 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
     5941 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
     6001 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
     6061 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
     6121 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
     6181 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
     6241 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
     6301 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
     6361 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
     6421 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
     6481 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
     6541 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
     6601 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa
     6661 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
     6721 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
     6781 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca
     6841 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
     6901 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg aattcgagct
     6961 ctaaggaggt tataaaaaat ggatattaat actgaaactg agatcaagca aaagcattca
     7021 ctaaccccct ttcctgtttt cctaatcagc ccggcatttc gcgggcgata ttttcacagc
     7081 tatttcagga gttcagccat gaacgcttat tacattcagg atcgtcttga ggctcagagc
     7141 tgggcgcgtc actaccagca gctcgcccgt gaagagaaag aggcagaact ggcagacgac
     7201 atggaaaaag gcctgcccca gcacctgttt gaatcgctat gcatcgatca tttgcaacgc
     7261 cacggggcca gcaaaaaatc cattacccgt gcgtttgatg acgatgttga gtttcaggag
     7321 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
     7381 gattcagagg tataaaacga atgagtactg cactcgcaac gctggctggg aagctggctg
     7441 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
     7501 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt actgatcgtt gccaaccagt
     7561 acggccttaa tccgtggacg aaagaaattt acgcctttcc tgataagcag aatggcatcg
     7621 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg
     7681 gcatggactt tgagcaggac aatgaatcct gtacatgccg gatttaccgc aaggaccgta
     7741 atcatccgat ctgcgttacc gaatggatgg atgaatgccg ccgcgaacca ttcaaaactc
     7801 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacgtcata
     7861 aagccatgat tcagtgtgcc cgtctggcct tcggatttgc tggtatctat gacaaggatg
     7921 aagccgagcg cattgtcgaa aatactgcat acactgcaga acgtcagccg gaacgcgaca
     7981 tcactccggt taacgatgaa accatgcagg agattaacac tctgctgatc gccctggata
     8041 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgtg
     8101 catcgtcaga actgacacag gccgaagcag taaaagctct tggattcctg aaacagaaag
     8161 ccgcagagca gaaggtggca gcatgacacc ggacattatc ctgcagcgta ccgggatcga
     8221 tgtgagagct gtcgaacagg gggatgatgc gtggcacaaa ttacggctcg gcgtcatcac
     8281 cgcttcagaa gttcacaacg tgatagcaaa accccgctcc ggaaagaagt ggcctgacat
     8341 gaaaatgtcc tacttccaca ccctgcttgc tgaggtttgc accggtgtgg ctccggaagt
     8401 taacgctaaa gcactggcct ggggaaaaca gtacgagaac gacgccagaa ccctgtttga
     8461 gttcacttcc ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     8521 taccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac tgaaatgccc
     8581 gtttacctcc cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaagtcagc
     8641 ttacatggcc caggtgcagt acagcatgtg ggtgacgcga aaaaatgcct ggtactttgc
     8701 caactatgac ccgcgtatga agcgtgaagg cctgcattat gtcgtgattg agcgggatga
     8761 aaagtacatg gcgagttttg acgagatcgt gccggagttc atcgaaaaaa tggacgaggc
     8821 actggctgaa attggttttg tatttgggga gcaatggcga tgacgcatcc tcacgataat
     8881 atccgggtag gcgcaatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     8941 ggcctttctg ttatccgaaa tccactgaaa gcacagcggc tggctgagga gataaataat
     9001 aaacgagggg ctgtatgcac aaagcatctt ctgttgagtt aagaacgagt atcgagatgg
     9061 cacatagcct tgctcaaatt ggaatcaggt ttgtgccaat accagtagaa acagacgaag
     9121 aatccatggt accgagctcg aattcgtaat cacccgggct caaaaaatac gcccggtagt
     9181 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     9241 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     9301 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     9361 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     9421 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     9481 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     9541 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     9601 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     9661 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     9721 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     9781 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     9841 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     9901 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     9961 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
    10021 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
    10081 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
    10141 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
    10201 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
    10261 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
    10321 ataaaatatt tgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
    10381 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
    10441 gcgtagagga tctgctcatg tttgacagct tatcatcgat gcataatgtg cctgtcaaat
    10501 ggacgaagca gggattctgc aaaccctatg ctacgttcat atgggattca cctttatgtt
    10561 gataagaaat aaaagaaaat gccaatagga tatcggcatt ttcttttgcg tttttatttg
    10621 ttaactgtta attgtccttg ttcaaggatg ctgtctttga caacagatgt tttcttgcct
    10681 ttgatgttca gcaggaagct aggcgcaaac gttgattgtt tgtctgcgta gaatcctctg
    10741 tttgtcatat agcttgtaat cacgacattg tttcctttcg cttgaggtac agcgaagtgt
    10801 gagtaagtaa aggttacatc gttaggatca agatccattt ttaacacaag gccagttttg
    10861 ttcagcggct tgtatgggcc agttaaagaa ttagaaacat aaccaagcat gtaaatatcg
    10921 ttagacgtaa tgccgtcaat cgtcattttt gatccgcggg agtcagtgaa cagataccat
    10981 ttgccgttca ttttaaagac gttcgcgcgt tcaatttcat ctgttactgt gttagatgca
    11041 atcagcggtt tcatcacttt tttcagtgtg taatcatcgt ttagctcaat cataccgaga
    11101 gcgccgtttg ctaactcagc cgtgcgtttt ttatcgcttt gcagaagttt ttgactttct
    11161 tgacggaaga atgatgtgct tttgccatag tatgctttgt taaataaaga ttcttcgcct
    11221 tggtagccat cttcagttcc agtgtttgct tcaaatacta agtatttgtg gcctttatct
    11281 tctacgtagt gaggatctct cagcgtatgg ttgtcgcctg agctgtagtt gccttcatcg
    11341 atgaactgct gtacattttg atacgttttt ccgtcaccgt caaagattga tttataatcc
    11401 tctacaccgt tgatgttcaa agagctgtct gatgctgata cgttaacttg tgcagttgtc
    11461 agtgtttgtt tgccgtaatg tttaccggag aaatcagtgt agaataaacg gatttttccg
    11521 tcagatgtaa atgtggctga acctgaccat tcttgtgttt ggtcttttag gatagaatca
    11581 tttgcatcga atttgtcgct gtctttaaag acgcggccag cgtttttcca gctgtcaata
    11641 gaagtttcgc cgactttttg atagaacatg taaatcgatg tgtcatccgc atttttagga
    11701 tctccggcta atgcaaagac gatgtggtag ccgtgatagt ttgcgacagt gccgtcagcg
    11761 ttttgtaatg gccagctgtc ccaaacgtcc aggccttttg cagaagagat atttttaatt
    11821 gtggacgaat cgaactcagg aacttgatat ttttcatttt tttgctgttc agggatttgc
    11881 agcatatcat ggcgtgtaat atgggaaatg ccgtatgttt ccttatatgg cttttggttc
    11941 gtttctttcg caaacgcttg agttgcgcct cctgccagca gtgcggtagt aaaggttaat
    12001 actgttgctt gttttgcaaa ctttttgatg ttcatcgttc atgtctcctt ttttatgtac
    12061 tgtgttagcg gtctgcttct tccagccctc ctgtttgaag atggcaagtt agttacgcac
    12121 aataaaaaaa gacctaaaat atgtaagggg tgacgccaaa gtatacactt tgccctttac
    12181 acattttagg tcttgcctgc tttatcagta acaaacccgc gcgatttact tttcgacctc
    12241 attctattag actctcgtca tcaaattaag cagaaggcca tcctgacgga tggccttttt
    12301 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    12361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    12421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    12481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    12541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    12601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
    12661 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    12721 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    12781 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    12841 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    12901 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
    12961 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    13021 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    13081 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    13141 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    13201 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    13261 gcattggtaa ctgtcagacc aagtttactc at
//
