LOCUS       pNL4-3-Luc-R-E-P       17878 bp DNA     circular SYN 01-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 2, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1290..1547
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             1703..3205
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2998..6009
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5694..5811
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8672..8905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9090..9134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9428..9454
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             9718..11364
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     CDS             11377..11433
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11458..12120
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             12205..12258
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             12259..12858
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             13042..13675
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(15486..16074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(16245..17105)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(17106..17210)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagcctaaag gtaaaaaata aaataataaa aaataaataa aatctagctc actccttcac
       61 atcaaaatgg agatacagct gttagcatta aataccaaat aacccatctt gtcctcaata
      121 attttaagcg cctctctcca ccacatctaa ctcctgtcaa aggcatgtgc cccttccggg
      181 cgctctgctg tgctgccaac caactggcat gtggactctg cagggtccct aactgccaag
      241 ccccacagtg tgccctgagg ctgccccttc cttctagcgg ctgcccccac tcggctttgc
      301 tttccctagt ttcagttact tgcgttcagc caaggtctga aactaggtgc gcacagagcg
      361 gtaagactgc gagagaaaga gaccagcttt acagggggtt tatcacagtg caccctgaca
      421 gtcgtcagcc tcacaggggg tttatcacat tgcaccctga cagtcgtcag cctcacaggg
      481 ggtttatcac agtgcaccct tacaatcatt ccatttgatt cacaattttt ttagtctcta
      541 ctgtgcctaa cttgtaagtt aaatttgatc agaggtgtgt tcccagaggg gaaaacagta
      601 tatacagggt tcagtactat cgcatttcag gcctccacct gggtcttgga atgtgtcccc
      661 cgaggggtga tgactacctc agttggatct ccacaggtca cagtgacaca agataaccaa
      721 gacacctccc aaggctacca caatgggccg ccctccacgt gcacatggcc ggaggaactg
      781 ccatgtcgga ggtgcaagca cacctgcgca tcagagtcct tggtgtggag ggagggacca
      841 gcgcagcttc cagccatcca cctgatgaac agaacctagg gaaagcccca gttctactta
      901 caccaggaaa ggctggaagg gctaatttgg tcccaaaaaa gacaagagat ccttgatctg
      961 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     1021 atcagatatc cactgacctt tggatggtgc ttcaagttag taccagttga accagagcaa
     1081 gtagaagagg ccaatgaagg agagaacaac agcttgttac accctatgag ccagcatggg
     1141 atggaggacc cggagggaga agtattagtg tggaagtttg acagcctcct agcatttcgt
     1201 cacatggccc gagagctgca tccggagtac tacaaagact gctgacatcg agctttctac
     1261 aagggacttt ccgctgggga ctttccaggg aggtgtggcc tgggcgggac tggggagtgg
     1321 cgagccctca gatgctacat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     1381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1441 ataaagcttg ccttgagtgc tcaaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1561 agggacttga aagcgaaagt aaagccagag gagatctctc gacgcaggac tcggcttgct
     1621 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1681 agcggaggct agaaggagag agatgggtgc gagagcgtcg gtattaagcg ggggagaatt
     1741 agataaatgg gaaaaaattc ggttaaggcc agggggaaag aaacaatata aactaaaaca
     1801 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc ttttagagac
     1861 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1921 agaacttaga tcattatata atacaatagc agtcctctat tgtgtgcatc aaaggataga
     1981 tgtaaaagac accaaggaag ccttagataa gatagaggaa gagcaaaaca aaagtaagaa
     2041 aaaggcacag caagcagcag ctgacacagg aaacaacagc caggtcagcc aaaattaccc
     2101 tatagtgcag aacctccagg ggcaaatggt acatcaggcc atatcaccta gaactttaaa
     2161 tgcatgggta aaagtagtag aagagaaggc tttcagccca gaagtaatac ccatgttttc
     2221 agcattatca gaaggagcca ccccacaaga tttaaatacc atgctaaaca cagtgggggg
     2281 acatcaagca gccatgcaaa tgttaaaaga gaccatcaat gaggaagctg cagaatggga
     2341 tagattgcat ccagtgcatg cagggcctat tgcaccaggc cagatgagag aaccaagggg
     2401 aagtgacata gcaggaacta ctagtaccct tcaggaacaa ataggatgga tgacacataa
     2461 tccacctatc ccagtaggag aaatctataa aagatggata atcctgggat taaataaaat
     2521 agtaagaatg tatagcccta ccagcattct ggacataaga caaggaccaa aggaaccctt
     2581 tagagactat gtagaccgat tctataaaac tctaagagcc gagcaagctt cacaagaggt
     2641 aaaaaattgg atgacagaaa ccttgttggt ccaaaatgcg aacccagatt gtaagactat
     2701 tttaaaagca ttgggaccag gagcgacact agaagaaatg atgacagcat gtcagggagt
     2761 ggggggaccc ggccataaag caagagtttt ggctgaagca atgagccaag taacaaatcc
     2821 agctaccata atgatacaga aaggcaattt taggaaccaa agaaagactg ttaagtgttt
     2881 caattgtggc aaagaagggc acatagccaa aaattgcagg gcccctagga aaaagggctg
     2941 ttggaaatgt ggaaaggaag gacaccaaat gaaagattgt actgagagac aggctaattt
     3001 tttagggaag atctggcctt cccacaaggg aaggccaggg aattttcttc agagcagacc
     3061 agagccaaca gccccaccag aagagagctt caggtttggg gaagagacaa caactccctc
     3121 tcagaagcag gagccgatag acaaggaact gtatccttta gcttccctca gatcactctt
     3181 tggcagcgac ccctcgtcac aataaagata ggggggcaat taaaggaagc tctattagat
     3241 acaggagcag atgatacagt attagaagaa atgaatttgc caggaagatg gaaaccaaaa
     3301 atgatagggg gaattggagg ttttatcaaa gtaagacagt atgatcagat actcatagaa
     3361 atctgcggac ataaagctat aggtacagta ttagtaggac ctacacctgt caacataatt
     3421 ggaagaaatc tgttgactca gattggctgc actttaaatt ttcccattag tcctattgag
     3481 actgtaccag taaaattaaa gccaggaatg gatggcccaa aagttaaaca atggccattg
     3541 acagaagaaa aaataaaagc attagtagaa atttgtacag aaatggaaaa ggaaggaaaa
     3601 atttcaaaaa ttgggcctga aaatccatac aatactccag tatttgccat aaagaaaaaa
     3661 gacagtacta aatggagaaa attagtagat ttcagagaac ttaataagag aactcaagat
     3721 ttctgggaag ttcaattagg aataccacat cctgcagggt taaaacagaa aaaatcagta
     3781 acagtactgg atgtgggcga tgcatatttt tcagttccct tagataaaga cttcaggaag
     3841 tatactgcat ttaccatacc tagtataaac aatgagacac cagggattag atatcagtac
     3901 aatgtgcttc cacagggatg gaaaggatca ccagcaatat tccagtgtag catgacaaaa
     3961 atcttagagc cttttagaaa acaaaatcca gacatagtca tctatcaata catggatgat
     4021 ttgtatgtag gatctgactt agaaataggg cagcatagaa caaaaataga ggaactgaga
     4081 caacatctgt tgaggtgggg atttaccaca ccagacaaaa aacatcagaa agaacctcca
     4141 ttcctttgga tgggttatga actccatcct gataaatgga cagtacagcc tatagtgctg
     4201 ccagaaaagg acagctggac tgtcaatgac atacagaaat tagtgggaaa attgaattgg
     4261 gcaagtcaga tttatgcagg gattaaagta aggcaattat gtaaacttct taggggaacc
     4321 aaagcactaa cagaagtagt accactaaca gaagaagcag agctagaact ggcagaaaac
     4381 agggagattc taaaagaacc ggtacatgga gtgtattatg acccatcaaa agacttaata
     4441 gcagaaatac agaagcaggg gcaaggccaa tggacatatc aaatttatca agagccattt
     4501 aaaaatctga aaacaggaaa gtatgcaaga atgaagggtg cccacactaa tgatgtgaaa
     4561 caattaacag aggcagtaca aaaaatagcc acagaaagca tagtaatatg gggaaagact
     4621 cctaaattta aattacccat acaaaaggaa acatgggaag catggtggac agagtattgg
     4681 caagccacct ggattcctga gtgggagttt gtcaataccc ctcccttagt gaagttatgg
     4741 taccagttag agaaagaacc cataatagga gcagaaactt tctatgtaga tggggcagcc
     4801 aatagggaaa ctaaattagg aaaagcagga tatgtaactg acagaggaag acaaaaagtt
     4861 gtccccctaa cggacacaac aaatcagaag actgagttac aagcaattca tctagctttg
     4921 caggattcgg gattagaagt aaacatagtg acagactcac aatatgcatt gggaatcatt
     4981 caagcacaac cagataagag tgaatcagag ttagtcagtc aaataataga gcagttaata
     5041 aaaaaggaaa aagtctacct ggcatgggta ccagcacaca aaggaattgg aggaaatgaa
     5101 caagtagata aattggtcag tgctggaatc aggaaagtac tatttttaga tggaatagat
     5161 aaggcccaag aagaacatga gaaatatcac agtaattgga gagcaatggc tagtgatttt
     5221 aacctaccac ctgtagtagc aaaagaaata gtagccagct gtgataaatg tcagctaaaa
     5281 ggggaagcca tgcatggaca agtagactgt agcccaggaa tatggcagct agattgtaca
     5341 catttagaag gaaaagttat cttggtagca gttcatgtag ccagtggata tatagaagca
     5401 gaagtaattc cagcagagac agggcaagaa acagcatact tcctcttaaa attagcagga
     5461 agatggccag taaaaacagt acatacagac aatggcagca atttcaccag tactacagtt
     5521 aaggccgcct gttggtgggc ggggatcaag caggaatttg gcattcccta caatccccaa
     5581 agtcaaggag taatagaatc tatgaataaa gaattaaaga aaattatagg acaggtaaga
     5641 gatcaggctg aacatcttaa gacagcagta caaatggcag tattcatcca caattttaaa
     5701 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     5761 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     5821 tacagggaca gcagagatcc agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa
     5881 ggggcagtag taatacaaga taatagtgac ataaaagtag tgccaagaag aaaagcaaag
     5941 atcatcaggg attatggaaa acagatggca ggtgatgatt gtgtggcaag tagacaggat
     6001 gaggattaac acatggaaaa gattagtaaa acaccatatg tatatttcaa ggaaagctaa
     6061 ggactggttt tatagacatc actatgaaag tactaatcca aaaataagtt cagaagtaca
     6121 catcccacta ggggatgcta aattagtaat aacaacatat tggggtctgc atacaggaga
     6181 aagagactgg catttgggtc agggagtctc catagaatgg aggaaaaaga gatatagcac
     6241 acaagtagac cctgacctag cagaccaact aattcatctg cactattttg attgtttttc
     6301 agaatctgct ataagaaata ccatattagg acgtatagtt agtcctaggt gtgaatatca
     6361 agcaggacat aacaaggtag gatctctaca gtacttggca ctagcagcat taataaaacc
     6421 aaaacagata aagccacctt tgcctagtgt taggaaactg acagaggaca gatggaacaa
     6481 gccccagaag accaagggcc acagagggag ccatacaatg aatggacact agagctttta
     6541 gaggaactta agagtgaagc tgttagacat tttcctagga tatggctcca taacttagga
     6601 caacatatct atgaaactta cggggatact tgggcaggag tggaagccat aataagaatt
     6661 ctgcaacaac tgctgtttat ccatttcaga attgggtgtc gacatagcag aataggcgtt
     6721 actcgacaga ggagagcaag aaatggagcc agtagatcct agactagagc cctggaagca
     6781 tccaggaagt cagcctaaaa ctgcttgtac caattgctat tgtaaaaagt gttgctttca
     6841 ttgccaagtt tgtttcatga caaaagcctt aggcatctcc tatggcagga agaagcggag
     6901 acagcgacga agagctcatc agaacagtca gactcatcaa gcttctctat caaagcagta
     6961 agtagtacat gtaatgcaac ctataatagt agcaatagta gcattagtag tagcaataat
     7021 aatagcaata gttgtgtggt ccatagtaat catagaatat aggaaaatat taagacaaag
     7081 aaaaatagac aggttaattg atagactaat agaaagagca gaagacagtg gcaatgagag
     7141 tgaaggagaa gtatcagcac ttgtggagat gggggtggaa atggggcacc atgctccttg
     7201 ggatattgat gatctgtagt gctacagaaa aattgtgggt cacagtctat tatggggtac
     7261 ctgtgtggaa ggaagcaacc accactctat tttgtgcatc agatgctaaa gcatatgata
     7321 cagaggtaca taatgtttgg gccacacatg cctgtgtacc cacagacccc aacccacaag
     7381 aagtagtatt ggtaaatgtg acagaaaatt ttaacatgtg gaaaaatgac atggtagaac
     7441 agatgcatga ggatataatc agtttatggg atcaaagcct aaagccatgt gtaaaattaa
     7501 ccccactctg tgttagttta aagtgcactg atttgaagaa tgatactaat accaatagta
     7561 gtagcgggag aatgataatg gagaaaggag agataaaaaa ctgctctttc aatatcagca
     7621 caagcataag agataaggtg cagaaagaat atgcattctt ttataaactt gatatagtac
     7681 caatagataa taccagctat aggttgataa gttgtaacac ctcagtcatt acacaggcct
     7741 gtccaaaggt atcctttgag ccaattccca tacattattg tgccccggct ggttttgcga
     7801 ttctaaaatg taataataag acgttcaatg gaacaggacc atgtacaaat gtcagcacag
     7861 tacaatgtac acatggaatc aggccagtag tatcaactca actgctgtta aatggcagtc
     7921 tagcagaaga agatgtagta attagatctg ccaatttcac agacaatgct aaaaccataa
     7981 tagtacagct gaacacatct gtagaaatta attgtacaag acccaacaac aatacaagaa
     8041 aaagtatccg tatccagagg ggaccaggga gagcatttgt tacaatagga aaaataggaa
     8101 atatgagaca agcacattgt aacattagta gagcaaaatg gaatgccact ttaaaacaga
     8161 tagctagcaa attaagagaa caatttggaa ataataaaac aataatcttt aagcaatcct
     8221 caggagggga cccagaaatt gtaacgcaca gttttaattg tggaggggaa tttttctact
     8281 gtaattcaac acaactgttt aatagtactt ggtttaatag tacttggagt actgaagggt
     8341 caaataacac tgaaggaagt gacacaatca cactcccatg cagaataaaa caatttataa
     8401 acatgtggca ggaagtagga aaagcaatgt atgcccctcc catcagtgga caaattagat
     8461 gttcatcaaa tattactggg ctgctattaa caagagatgg tggtaataac aacaatgggt
     8521 ccgagatctt cagacctgga ggaggcgata tgagggacaa ttggagaagt gaattatata
     8581 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     8641 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     8701 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     8761 tgtctgatat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     8821 tgttgcaact cacagtctgg ggcatcaaac agctccaggc aagaatcctg gctgtggaaa
     8881 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     8941 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaata
     9001 acatgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9061 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     9121 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     9181 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     9241 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaatcccga
     9301 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     9361 ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg agcctgtgcc
     9421 tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt gtggaacttc
     9481 tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa tattggagtc
     9541 aggagctaaa gaatagtgct gttagcttgc tcaatgccac agccatagca gtagctgagg
     9601 ggacagatag ggttatagaa gtagtacaag gagcttgtag agctattcgc cacataccta
     9661 gaagaataag acagggcttg gaaaggattt tgctataaga tgggtggcgc ggccgctgaa
     9721 gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct
     9781 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     9841 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
     9901 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     9961 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
    10021 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
    10081 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
    10141 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
    10201 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
    10261 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact
    10321 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
    10381 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
    10441 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
    10501 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
    10561 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
    10621 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
    10681 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
    10741 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
    10801 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
    10861 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
    10921 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
    10981 atagcttact gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
    11041 aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc
    11101 aacatcttcg acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc
    11161 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
    11221 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
    11281 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
    11341 aagaagggcg gaaagtccaa attgctcgag agcggagcta ctaacttcag cctgctgaag
    11401 caggctggag acgtggagga gaaccctgga cctatggaga gcgacgagag cggcctgccc
    11461 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg
    11521 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa
    11581 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac
    11641 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc
    11701 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc
    11761 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc
    11821 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg
    11881 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac
    11941 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc
    12001 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc
    12061 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat tgccttcgcc
    12121 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc
    12181 ggatctcgcg tcgacggcag tggagagggc agaggaagtc tgctaacatg cggtgacgtc
    12241 gaggagaatc ctggcccaat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
    12301 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
    12361 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
    12421 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
    12481 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
    12541 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
    12601 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
    12661 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    12721 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
    12781 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    12841 cgcaagcccg gtgcctgaac ctagaaaaac atggagcaat cacaagtagc aatacagcag
    12901 ctaacaatgc tgcttgtgcc tggctagaag cacaagagga ggaagaggtg ggttttccag
    12961 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
    13021 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaaagaaga caagatatcc
    13081 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
    13141 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    13201 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    13261 tgcatggaat ggatgaccct gagagagaag tgttagagtg gaggtttgac agccgcctag
    13321 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgacatcgag
    13381 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
    13441 gggagtggcg agccctcaga tgctgcatat aagcagctgc tttttgcctg tactgggtct
    13501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    13561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    13621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcacccag
    13681 gaggtagagg ttgcagtgag ccaagatcgc gccactgcat tccagcctgg gcaagaaaac
    13741 aagactgtct aaaataataa taataagtta agggtattaa atatatttat acatggaggt
    13801 cataaaaata tatatatttg ggctgggcgc agtggctcac acctgcgccc ggccctttgg
    13861 gaggccgagg caggtggatc acctgagttt gggagttcca gaccagcctg accaacatgg
    13921 agaaacccct tctctgtgta tttttagtag attttatttt atgtgtattt tattcacagg
    13981 tatttctgga aaactgaaac tgtttttcct ctactctgat accacaagaa tcatcagcac
    14041 agaggaagac ttctgtgatc aaatgtggtg ggagagggag gttttcacca gcacatgagc
    14101 agtcagttct gccgcagact cggcgggtgt ccttcggttc agttccaaca ccgcctgcct
    14161 ggagagaggt cagaccacag ggtgagggct cagtccccaa gacataaaca cccaagacat
    14221 aaacacccaa caggtccacc ccgcctgctg cccaggcaga gccgattcac caagacggga
    14281 attaggatag agaaagagta agtcacacag agccggctgt gcgggagaac ggagttctat
    14341 tatgactcaa atcagtctcc ccaagcattc ggggatcaga gtttttaagg ataacttagt
    14401 gtgtaggggg ccagtgagtt ggagatgaaa gcgtagggag tcgaaggtgt ccttttgcgc
    14461 cgagtcagtt cctgggtggg ggccacaaga tcggatgagc cagtttatca atccgggggt
    14521 gccagctgat ccatggagtg cagggtctgc aaaatatctc aagcactgat tgatcttagg
    14581 ttttacaata gtgatgttac cccaggaaca atttggggaa ggtcagaatc ttgtagcctg
    14641 tagctgcatg actcctaaac cataatttct tttttgtttt ttttttttta tttttgagac
    14701 agggtctcac tctgtcacct aggctggagt gcagtggtgc aatcacagct cactgcagcc
    14761 tcaacgtcgt aagctcaagc gatcctccca cctcagcctg cctggtagct gagactacaa
    14821 gcgacgcccc agttaatttt tgtatttttg gtagaggcag cgttttgccg tgtggccctg
    14881 gctggtctcg aactcctggg ctcaagtgat ccagcctcag cctcccaaag tgctgggaca
    14941 accggggcca gtcactgcac ctggccctaa accataattt ctaatctttt ggctaatttg
    15001 ttagtcctac aaaggcagtc tagtccccag gcaaaaaggg ggtttgtttc gggaaagggc
    15061 tgttactgtc tttgtttcaa actataaact aagttcctcc taaacttagt tcggcctaca
    15121 cccaggaatg aacaaggaga gcttggaggt tagaagcacg atggaattgg ttaggtcaga
    15181 tctctttcac tgtctgagtt ataattttgc aatggtggtt caaagactgc ccgcttctga
    15241 caccagtcgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    15301 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    15361 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    15421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    15481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    15541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    15601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    15661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    15721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    15781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    15841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    15901 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    15961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    16021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    16081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    16141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    16201 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    16261 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    16321 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    16381 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    16441 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    16501 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    16561 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    16621 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    16681 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    16741 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    16801 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    16861 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    16921 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    16981 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    17041 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    17101 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    17161 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    17221 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    17281 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    17341 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    17401 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    17461 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    17521 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    17581 cgatcggtgc gggcctcttc gctattacgc caggggaggc agagattgca gtaagctgag
    17641 atcgcagcac tgcactccag cctgggcgac agagtaagac tctgtctcaa aaataaaata
    17701 aataaatcaa tcagatattc caatcttttc ctttatttat ttatttattt tctattttgg
    17761 aaacacagtc cttccttatt ccagaattac acatatattc tatttttctt tatatgctcc
    17821 agtttttttt agaccttcac ctgaaatgtg tgtatacaaa atctaggcca gtccagca
//
