LOCUS       pNL3.1[Nluc_minP        3151 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using a NanoLuc(R) luciferase 
            assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.1[Nluc minP].
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..653
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(705..826)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1245..1833)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2036..2893)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    2998..3046
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3060..3151
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggtcttca cactcgaaga tttcgttggg gactggcgac
      181 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
      241 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
      301 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
      361 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
      421 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
      481 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
      541 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
      601 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaaggcc
      661 gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
      721 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
      781 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
      841 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
      901 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
      961 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     1021 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     1081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1981 tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt
     2041 gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct
     2101 gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag
     2161 caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag
     2221 cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga
     2281 gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg
     2341 ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg
     2401 gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct
     2461 ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa
     2521 tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg
     2581 gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc
     2641 cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg
     2701 ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga
     2761 tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg
     2821 ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat
     2881 gttggatgct catactcgtc ctttttcaat attattgaag catttatcag ggttactagt
     2941 acgtctctca aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat
     3001 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     3061 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     3121 agtgcaagtg caggtgccag aacatttctc t
//
