LOCUS       pNL2.2[NlucP_Hyg        4832 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding destabilized NanoLuc(R) luciferase for 
            measuring the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pNL2.2[NlucP Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..612
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     CDS             616..735
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(787..908)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1102..1459
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1490..2524
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2551..2599
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2926..3514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3717..4574)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4679..4727
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4741..4832
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
      121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
      181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
      241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
      301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
      361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
      421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
      481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
      541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
      601 cgcattctgg cgaattctca cggctttccg cctgaggttg aagagcaagc cgccggtaca
      661 ttgcctatgt cctgcgcaca agaaagcggt atggaccggc acccagccgc ttgtgcttca
      721 gctcgcatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
      781 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      841 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      901 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      961 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
     1021 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
     1081 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     1141 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1201 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1261 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     1321 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1381 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     1441 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaagaagcc
     1501 cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag aagttcgaca gtgtgagcga
     1561 cctgatgcag ttgtcggagg gcgaagagag ccgagccttc agcttcgatg tcggcggacg
     1621 cggctatgta ctgcgggtga atagctgcgc tgatggcttc tacaaagacc gctacgtgta
     1681 ccgccacttc gccagcgctg cactacccat ccccgaagtg ttggacatcg gcgagttcag
     1741 cgagagcctg acatactgca tcagtagacg cgcccaaggc gttactctcc aagacctccc
     1801 cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa gctatggatg ctattgccgc
     1861 cgccgacctc agtcaaacca gcggcttcgg cccattcggg ccccaaggca tcggccagta
     1921 cacaacctgg cgggatttca tttgcgccat tgctgatccc catgtctacc actggcagac
     1981 cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc ctggacgaac tgatgctgtg
     2041 ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc gacttcggca gcaacaacgt
     2101 cctgaccgac aacggccgca tcaccgccgt aatcgactgg tccgaagcta tgttcgggga
     2161 cagtcagtac gaggtggcca acatcttctt ctggcggccc tggctggctt gcatggagca
     2221 gcagactcgc tacttcgagc gccggcatcc cgagctggcc ggcagccctc gtctgcgagc
     2281 ctacatgctg cgcatcggcc tggatcagct ctaccagagc ctcgtggacg gcaacttcga
     2341 cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc cgcagcgggg ccggcaccgt
     2401 cggtcgcaca caaatcgctc gccggagcgc agccgtatgg accgacggct gcgtcgaggt
     2461 gctggccgac agcggcaacc gccggcccag tacacgaccg cgcgctaagg aggtaggtcg
     2521 agtttaaact ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg
     2581 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc
     2641 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     2701 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct
     2761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3661 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag
     3721 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc
     3781 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca
     3841 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca
     3901 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg
     3961 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg
     4021 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct
     4081 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc
     4141 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta
     4201 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc
     4261 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc
     4321 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc
     4381 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg
     4441 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg
     4501 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa
     4561 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag
     4621 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa
     4681 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     4741 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     4801 cagtgcaagt gcaggtgcca gaacatttct ct
//
