LOCUS       pNL1.3.CMV[secNl        3945 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Co-transfection vector for expressing secreted NanoLuc(R) luciferase
            under control of the strong CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pNL1.3.CMV[secNluc CMV].
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             859..945
                     /codon_start=1
                     /product="secretion signal from interleukin 6"
                     /label=secretion signal from interleukin 6
                     /note="IL-6 signal sequence"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
     CDS             946..1455
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(1499..1620)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2039..2627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2830..3687)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3792..3840
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3854..3945
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
       61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
      781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
      841 actgttggta aagccaccat gaactccttc tccacaagcg ccttcggtcc agttgccttc
      901 tccctgggcc tgctcctggt gttgcctgct gccttccctg ccccagtctt cacactcgaa
      961 gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag
     1021 ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt
     1081 gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt
     1141 ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat
     1201 gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg
     1261 aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag
     1321 atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac
     1381 cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc
     1441 gaacgcattc tggcgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     1501 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1561 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     1621 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     1681 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     1741 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     1801 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     1861 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1921 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1981 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2041 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2101 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2161 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2221 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2281 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2341 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2401 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2461 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2521 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2581 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2641 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2701 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2761 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     2821 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     2881 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     2941 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     3001 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     3061 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     3121 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     3181 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     3241 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     3301 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     3361 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     3421 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     3481 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     3541 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     3601 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     3661 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     3721 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     3781 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     3841 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     3901 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
