LOCUS       pNL1.3[secNluc].        3194 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a secreted NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL1.3[secNluc]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..186
                     /codon_start=1
                     /product="secretion signal from interleukin 6"
                     /label=secretion signal from interleukin 6
                     /note="IL-6 signal sequence"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
     CDS             187..696
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(748..869)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1288..1876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2079..2936)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3041..3089
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3103..3194
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tgaactcctt ctccacaagc
      121 gccttcggtc cagttgcctt ctccctgggc ctgctcctgg tgttgcctgc tgccttccct
      181 gccccagtct tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac
      241 ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc
      301 gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat
      361 gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt
      421 aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg
      481 gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc
      541 gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt
      601 atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga
      661 gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag gccgcgactc tagagtcggg
      721 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
      781 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      841 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      901 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      961 aaatcgataa ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     1021 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     1081 ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1981 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2041 tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat cagtgaggca
     2101 ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc cgtcgtgtag
     2161 atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat gccgcgagag
     2221 ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag ggccgagcga
     2281 agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg tcgtgatgct
     2341 agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc tactggcatc
     2401 gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca gcggtcaagc
     2461 cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg gcctccgatc
     2521 gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc actacacaat
     2581 tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag
     2641 tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc tatacgggac
     2701 aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg
     2761 cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc cactcttgca
     2821 cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc aaaaacaggc
     2881 aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat gctcatactc
     2941 gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc tcaaggataa
     3001 gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat ctttattttc
     3061 attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca
     3121 aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc
     3181 cagaacattt ctct
//
