LOCUS       pNL1.1[Nluc].        3110 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL1.1[Nluc].
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..612
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(664..785)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1204..1792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1995..2852)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    2957..3005
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3019..3110
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
      121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
      181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
      241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
      301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
      361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
      421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
      481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
      541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
      601 cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
      661 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      721 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      781 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      841 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
      901 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
      961 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     1021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
     1981 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
     2041 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
     2101 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
     2161 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
     2221 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
     2281 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
     2341 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
     2401 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
     2461 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
     2521 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
     2581 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
     2641 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
     2701 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
     2761 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
     2821 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
     2881 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
     2941 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     3001 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     3061 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
