LOCUS       40924_33202        6147 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pNIA-Cc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Zaltsman A, Yi BY, Krichevsky A, Gafni Y, Citovsky V.
  TITLE     Yeast-plant coupled vector system for identification of nuclear 
            proteins
  JOURNAL   Plant Physiol. 145 (4), 1264-1271 (2007)
  PUBMED    17704231
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Zaltsman AA, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2007) Biochemistry and Cell Bio, State University 
            of New York, Stony Brook, NY 11777, USA
REFERENCE   3  (bases 1 to 6147)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6147)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1264-1271"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-APR-2007) Biochemistry and Cell Bio, State University of New 
            York, Stony Brook, NY 11777, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..630
                     /codon_start=1
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
                     /translation="MKALTARQQEVFDLIHDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKGLEKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             637..975
                     /codon_start=1
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
                     /translation="NFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNV
                     HDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDE
                     DTPPNPKKE"
     misc_feature    996..1061
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1185..1306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1380..2051)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2052..2153)
                     /label=TRP1 promoter
     primer_bind     complement(2167..2183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2191..2207)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2215..2245)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2260..2281)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2569..3157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3331..4188)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEAS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4189..4293)
                     /label=AmpR promoter
     rep_origin      4575..5739
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5749..6145
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 aagcttgaat taattccggg cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt
       61 gatctcatcc atgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
      121 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
      181 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag
      241 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct ggcgcaacag
      301 catattgaag gtcattatca ggtcgatcct tccttattca agccgaatgc tgatttcctg
      361 ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga cttgctggca
      421 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgag
      481 gttaccgtta agggcctgga aaaacagggc aataaagtcg aactgttgcc agaaaatagc
      541 gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg
      601 gttggggtta ttcgcaacgg cgactggctg gaattcaatt ttaatcaaag tgggaatatt
      661 gctgatagct cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca
      721 actcaaacaa attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac
      781 ttcatgaata atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg
      841 tcacctggtt ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt
      901 aataccacta caatggatga tgtatataac tatctattcg atgatgaaga taccccacca
      961 aacccaaaaa aagagatcga atacccggtg tacagtccgg actcagatct cgagctcaag
     1021 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
     1081 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     1141 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     1201 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1261 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     1321 agcggccgcc ggcaagtgca caaacaatac ttaaataaat actactcagt aataacctat
     1381 ttcttagcat ttttgacgaa atttgctatt ttgttagagt cttttacacc atttgtctcc
     1441 acacctccgc ttacatcaac accaataacg ccatttaatc taagcgcatc accaacattt
     1501 tctggcgtca gtccaccagc taacataaaa tgtaagcttt cggggctctc ttgccttcca
     1561 acccagtcag aaatcgagtt ccaatccaaa agttcacctg tcccacctgc ttctgaatca
     1621 aacaagggaa taaacgaatg aggtttctgt gaagctgcac tgagtagtat gttgcagtct
     1681 tttggaaata cgagtctttt aataactggc aaaccgagga actcttggta ttcttgccac
     1741 gactcatctc catgcagttg gacgatatca atgccgtaat cattgaccag agccaaaaca
     1801 tcctccttag gttgattacg aaacacgcca accaagtatt tcggagtgcc tgaactattt
     1861 ttatatgctt ttacaagact tgaaattttc cttgcaataa ccgggtcaat tgttctcttt
     1921 ctattgggca cacatataat acccagcaag tcagcatcgg aatctagagc acattctgcg
     1981 gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc tgtgaaatta
     2041 ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca cgtgctcaat
     2101 agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga attaattcgt
     2161 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2221 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2341 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2401 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2461 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2521 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2581 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2641 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2701 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2761 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2821 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2881 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2941 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3001 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3061 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3121 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3181 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3241 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag gcctcaacca agtcattctg
     3901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     3961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4321 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4381 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4441 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4501 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     4561 gagagtgcac cataacgcat ttaagcataa acacgcacta tgccgttctt ctcatgtata
     4621 tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg tatgtgcgca
     4681 gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt cctattccga
     4741 agttcctatt ctctagctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc
     4801 gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa
     4861 tattgcgaat accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg
     4921 tgctatatcc ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact
     4981 cgacctctac attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt
     5041 aagaactatt catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgta
     5101 gtatatagag acaaaataga agaaaccgtt cataattttc tgaccaatga agaatcatca
     5161 acgctatcac tttctgttca caaagtatgc gcaatccaca tcggtataga atataatcgg
     5221 ggatgccttt atcttgaaaa aatgcacccg cagcttcgct agtaatcagt aaacgcggga
     5281 agtggagtca ggcttttttt atggaagaga aaatagacac caaagtagcc ttcttctaac
     5341 cttaacggac ctacagtgca aaaagttatc aagagactgc attatagagc gcacaaagga
     5401 gaaaaaaagt aatctaagat gctttgttag aaaaatagcg ctctcgggat gcatttttgt
     5461 agaacaaaaa agaagtatag attctttgtt ggtaaaatag cgctctcgcg ttgcatttct
     5521 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt
     5581 tttgttttac aaaaatgaag cacagattct tcgttggtaa aatagcgctt tcgcgttgca
     5641 tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt
     5701 gcatttttgt tctacaaaat gaagcacaga tgcttcgttg cttgcatgca acttcttttc
     5761 tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa
     5821 tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt
     5881 tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc
     5941 acactactct ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat
     6001 aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg
     6061 tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca
     6121 agctatacca agcatacaat caactcc
//
