LOCUS       40924_33197        6149 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pNIA-Cb, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Zaltsman A, Yi BY, Krichevsky A, Gafni Y, Citovsky V.
  TITLE     Yeast-plant coupled vector system for identification of nuclear 
            proteins
  JOURNAL   Plant Physiol. 145 (4), 1264-1271 (2007)
  PUBMED    17704231
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Zaltsman AA, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2007) Biochemistry and Cell Bio, State University 
            of New York, Stony Brook, NY 11777, USA
REFERENCE   3  (bases 1 to 6149)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6149)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1264-1271"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-APR-2007) Biochemistry and Cell Bio, State University of New 
            York, Stony Brook, NY 11777, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..630
                     /codon_start=1
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
                     /translation="MKALTARQQEVFDLIHDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKGLEKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             637..975
                     /codon_start=1
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
                     /translation="NFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNV
                     HDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDE
                     DTPPNPKKE"
     misc_feature    998..1063
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1187..1308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1382..2053)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2054..2155)
                     /label=TRP1 promoter
     primer_bind     complement(2169..2185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2193..2209)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2217..2247)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2262..2283)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2571..3159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3333..4190)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEAS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4191..4295)
                     /label=AmpR promoter
     rep_origin      4577..5741
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5751..6147
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 aagcttgaat taattccggg cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt
       61 gatctcatcc atgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
      121 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
      181 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag
      241 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct ggcgcaacag
      301 catattgaag gtcattatca ggtcgatcct tccttattca agccgaatgc tgatttcctg
      361 ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga cttgctggca
      421 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgag
      481 gttaccgtta agggcctgga aaaacagggc aataaagtcg aactgttgcc agaaaatagc
      541 gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg
      601 gttggggtta ttcgcaacgg cgactggctg gaattcaatt ttaatcaaag tgggaatatt
      661 gctgatagct cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca
      721 actcaaacaa attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac
      781 ttcatgaata atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg
      841 tcacctggtt ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt
      901 aataccacta caatggatga tgtatataac tatctattcg atgatgaaga taccccacca
      961 aacccaaaaa aagagatcga atacccggtg tacaaagtcc ggactcagat ctcgagctca
     1021 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
     1081 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1141 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     1321 taagcggccg ccggcaagtg cacaaacaat acttaaataa atactactca gtaataacct
     1381 atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct
     1441 ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat
     1501 tttctggcgt cagtccacca gctaacataa aatgtaagct ttcggggctc tcttgccttc
     1561 caacccagtc agaaatcgag ttccaatcca aaagttcacc tgtcccacct gcttctgaat
     1621 caaacaaggg aataaacgaa tgaggtttct gtgaagctgc actgagtagt atgttgcagt
     1681 cttttggaaa tacgagtctt ttaataactg gcaaaccgag gaactcttgg tattcttgcc
     1741 acgactcatc tccatgcagt tggacgatat caatgccgta atcattgacc agagccaaaa
     1801 catcctcctt aggttgatta cgaaacacgc caaccaagta tttcggagtg cctgaactat
     1861 ttttatatgc ttttacaaga cttgaaattt tccttgcaat aaccgggtca attgttctct
     1921 ttctattggg cacacatata atacccagca agtcagcatc ggaatctaga gcacattctg
     1981 cggcctctgt gctctgcaag ccgcaaactt tcaccaatgg accagaacta cctgtgaaat
     2041 taataacaga catactccaa gctgcctttg tgtgcttaat cacgtatact cacgtgctca
     2101 atagtcacca atgccctccc tcttggccct ctccttttct tttttcgacc gaattaattc
     2161 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2221 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2281 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2341 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2401 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3301 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3841 actgtcatgc catccgtaag atgcttttct gtgactggtg aggcctcaac caagtcattc
     3901 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3961 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4021 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4081 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4141 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4261 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4321 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4381 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     4441 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     4501 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     4561 ctgagagtgc accataacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta
     4621 tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg
     4681 cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc
     4741 gaagttccta ttctctagct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa
     4801 gcgctctgaa gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa
     4861 aatattgcga ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc
     4921 tgtgctatat ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa
     4981 ctcgacctct acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta
     5041 gtaagaacta ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg
     5101 tagtatatag agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat
     5161 caacgctatc actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc
     5221 ggggatgcct ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg
     5281 gaagtggagt caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta
     5341 accttaacgg acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag
     5401 gagaaaaaaa gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt
     5461 gtagaacaaa aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt
     5521 ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca
     5581 tttttgtttt acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg
     5641 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg
     5701 ttgcattttt gttctacaaa atgaagcaca gatgcttcgt tgcttgcatg caacttcttt
     5761 tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca atgacaaaaa
     5821 aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt
     5881 gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc cttcattcac
     5941 gcacactact ctctaatgag caacggtata cggccttcct tccagttact tgaatttgaa
     6001 ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt attaatcttt
     6061 tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg cacaatattt
     6121 caagctatac caagcataca atcaactcc
//
