LOCUS       40924_33192        6151 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pNIA-Ca, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Zaltsman A, Yi BY, Krichevsky A, Gafni Y, Citovsky V.
  TITLE     Yeast-plant coupled vector system for identification of nuclear 
            proteins
  JOURNAL   Plant Physiol. 145 (4), 1264-1271 (2007)
  PUBMED    17704231
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Zaltsman AA, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2007) Biochemistry and Cell Bio, State University 
            of New York, Stony Brook, NY 11777, USA
REFERENCE   3  (bases 1 to 6151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1264-1271"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-APR-2007) Biochemistry and Cell Bio, State University of New 
            York, Stony Brook, NY 11777, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..630
                     /codon_start=1
                     /label=LexA
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
                     /translation="MKALTARQQEVFDLIHDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKGLEKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             637..975
                     /codon_start=1
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
                     /translation="NFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSNV
                     HDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDDE
                     DTPPNPKKE"
     misc_feature    997..1062
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1186..1307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1384..2055)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(2056..2157)
                     /label=TRP1 promoter
     primer_bind     complement(2171..2187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2195..2211)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2219..2249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2264..2285)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2573..3161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3335..4192)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEAS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4193..4297)
                     /label=AmpR promoter
     rep_origin      4579..5743
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5753..6149
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 aagcttgaat taattccggg cggaatgaaa gcgttaacgg ccaggcaaca agaggtgttt
       61 gatctcatcc atgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
      121 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
      181 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga ttcgtctgtt gcaggaagag
      241 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct ggcgcaacag
      301 catattgaag gtcattatca ggtcgatcct tccttattca agccgaatgc tgatttcctg
      361 ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga cttgctggca
      421 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgag
      481 gttaccgtta agggcctgga aaaacagggc aataaagtcg aactgttgcc agaaaatagc
      541 gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg
      601 gttggggtta ttcgcaacgg cgactggctg gaattcaatt ttaatcaaag tgggaatatt
      661 gctgatagct cattgtcctt cactttcact aacagtagca acggtccgaa cctcataaca
      721 actcaaacaa attctcaagc gctttcacaa ccaattgcct cctctaacgt tcatgataac
      781 ttcatgaata atgaaatcac ggctagtaaa attgatgatg gtaataattc aaaaccactg
      841 tcacctggtt ggacggacca aactgcgtat aacgcgtttg gaatcactac agggatgttt
      901 aataccacta caatggatga tgtatataac tatctattcg atgatgaaga taccccacca
      961 aacccaaaaa aagagatcga atacccggtg tacaagtccg gactcagatc tcgagctcaa
     1021 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg
     1081 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1141 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
     1321 aagcggccgc cggcggcaag tgcacaaaca atacttaaat aaatactact cagtaataac
     1381 ctatttctta gcatttttga cgaaatttgc tattttgtta gagtctttta caccatttgt
     1441 ctccacacct ccgcttacat caacaccaat aacgccattt aatctaagcg catcaccaac
     1501 attttctggc gtcagtccac cagctaacat aaaatgtaag ctttcggggc tctcttgcct
     1561 tccaacccag tcagaaatcg agttccaatc caaaagttca cctgtcccac ctgcttctga
     1621 atcaaacaag ggaataaacg aatgaggttt ctgtgaagct gcactgagta gtatgttgca
     1681 gtcttttgga aatacgagtc ttttaataac tggcaaaccg aggaactctt ggtattcttg
     1741 ccacgactca tctccatgca gttggacgat atcaatgccg taatcattga ccagagccaa
     1801 aacatcctcc ttaggttgat tacgaaacac gccaaccaag tatttcggag tgcctgaact
     1861 atttttatat gcttttacaa gacttgaaat tttccttgca ataaccgggt caattgttct
     1921 ctttctattg ggcacacata taatacccag caagtcagca tcggaatcta gagcacattc
     1981 tgcggcctct gtgctctgca agccgcaaac tttcaccaat ggaccagaac tacctgtgaa
     2041 attaataaca gacatactcc aagctgcctt tgtgtgctta atcacgtata ctcacgtgct
     2101 caatagtcac caatgccctc cctcttggcc ctctcctttt cttttttcga ccgaattaat
     2161 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2221 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2281 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2341 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2401 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2461 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2521 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2581 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2641 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2701 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2761 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2821 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2881 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2941 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3001 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3061 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3121 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3181 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3241 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3301 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3361 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3421 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3481 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3541 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3601 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3661 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3721 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3781 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3841 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgaggcctca accaagtcat
     3901 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3961 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4321 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4381 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4441 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4501 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     4561 tactgagagt gcaccataac gcatttaagc ataaacacgc actatgccgt tcttctcatg
     4621 tatatatata tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg
     4681 cgcagctcgc gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt
     4741 ccgaagttcc tattctctag ctagaaagta taggaacttc agagcgcttt tgaaaaccaa
     4801 aagcgctctg aagacgcact ttcaaaaaac caaaaacgca ccggactgta acgagctact
     4861 aaaatattgc gaataccgct tccacaaaca ttgctcaaaa gtatctcttt gctatatatc
     4921 tctgtgctat atccctatat aacctaccca tccacctttc gctccttgaa cttgcatcta
     4981 aactcgacct ctacattttt tatgtttatc tctagtatta ctctttagac aaaaaaattg
     5041 tagtaagaac tattcataga gtgaatcgaa aacaatacga aaatgtaaac atttcctata
     5101 cgtagtatat agagacaaaa tagaagaaac cgttcataat tttctgacca atgaagaatc
     5161 atcaacgcta tcactttctg ttcacaaagt atgcgcaatc cacatcggta tagaatataa
     5221 tcggggatgc ctttatcttg aaaaaatgca cccgcagctt cgctagtaat cagtaaacgc
     5281 gggaagtgga gtcaggcttt ttttatggaa gagaaaatag acaccaaagt agccttcttc
     5341 taaccttaac ggacctacag tgcaaaaagt tatcaagaga ctgcattata gagcgcacaa
     5401 aggagaaaaa aagtaatcta agatgctttg ttagaaaaat agcgctctcg ggatgcattt
     5461 ttgtagaaca aaaaagaagt atagattctt tgttggtaaa atagcgctct cgcgttgcat
     5521 ttctgttctg taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg
     5581 catttttgtt ttacaaaaat gaagcacaga ttcttcgttg gtaaaatagc gctttcgcgt
     5641 tgcatttctg ttctgtaaaa atgcagctca gattctttgt ttgaaaaatt agcgctctcg
     5701 cgttgcattt ttgttctaca aaatgaagca cagatgcttc gttgcttgca tgcaacttct
     5761 tttctttttt tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa
     5821 aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg
     5881 ttgttccaga gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc
     5941 acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg
     6001 aaataaaaaa aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct
     6061 tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat
     6121 ttcaagctat accaagcata caatcaactc c
//
