LOCUS       V004330                 9791 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004330
VERSION     V004330
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Pisciotta
            A, Alduina R, Manteca A.
  TITLE     New PhiBT1 site-specific integrative vectors with neutral phenotype
            in Streptomyces
  JOURNAL   Appl. Microbiol. Biotechnol. 100 (6), 2797-2808 (2016)
   PUBMED   26758297
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Manteca
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2015) Biologia Funcional, Universidad de Oviedo,
            c/Julian Claveria s/n Facultad de Medicina, Oviedo 33006, Spain
REFERENCE   3  (bases 1 to 9791)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2016"; volume: "100"; issue: "6";
            pages: "2797-2808"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-APR-2015) Biologia Funcional, Universidad de Oviedo, c/Julian
            Claveria s/n Facultad de Medicina, Oviedo 33006, Spain"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(120..977)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(978..1041)
                     /label="AmpR promoter"
     rep_origin      1310..1898
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2186..2207
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2222..2252
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2260..2276
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2284..2300
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      2463..2511
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      complement(2822..2825)
                     /regulatory_class="ribosome_binding_site"
     oriT            5097..5206
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             5239..5607
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(6323..7318)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     CDS             complement(8003..9313)
                     /codon_start=1
                     /gene="SCO4849"
                     /product="SCO4849 protein"
                     /label="SCO4849"
                     /protein_id="AML61769.1"
                     /translation="MVVVFVLVALLMVGVLVTANWYVWRRLFRDTTRAPGPVRRIGAAV
                     IAGGWLLAVGALVAERAGAPFWLQRVLAWPGFLWLALSIYLLLAVLAGEVVRPLLRRFL
                     ERRAAARRTAGAEPSVTPAADTSRIPAGTAPPKTPEAPETPEAPGTEALAAQGSPLAVP
                     SRRLFVSRVVAGAAAAAAVGTVGYGTYGVLSGPKVKRVTVPLAKLPRAAHGFRIAVVSD
                     IHLGPVLGRGFAQQVVDTINSTQPDLIAVVGDLVDGSVKDLGPAAAPLARLTARHGAYF
                     VTGNHEYFSGAEQWVAEVRRLGLLPLENARTELPHFDLAGVNDVAGEDEGQGPDYDRAL
                     GDRDRSRACVLLAHQPVQIHDAVDHGVDLQLSGHTHGGQLWPGNLIAGAANPTLAGLER
                     YGDTQLYVSRGAGAWGPPTRVGAPSDITVIELASRQA"
     gene            complement(8003..9313)
                     /gene="SCO4849"
                     /label="SCO4849"
     regulatory      complement(9368..9790)
                     /regulatory_class="promoter"
ORIGIN
        1 agcttaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
       61 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      421 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      721 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
      781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1021 tgtctcatga gcggatacat atttcctagg ttctcggctt ttcgccattc gtattgcacg
     1081 acattgcact ccaccgctga tgacatcagt cgatcatagc acgatcaacg gcactgttgc
     1141 aaatagtcgg tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaacca
     1201 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1261 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1321 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1381 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1441 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1501 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1561 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1621 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1681 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1741 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1801 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1861 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1921 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1981 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2041 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2101 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2161 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2221 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2281 acacaggaaa cagctatgac atgattacga attcgatatc tcccgcaaaa gcggcctttg
     2341 actccctgca agcctcagcg accgaatata tcggttatgc gtgggcgatg gttgttgtca
     2401 ttgtcggcgc aactatcggt atcaagctgt ttaagaaatt cacctcgaaa gcaagctgat
     2461 aaaccgatac aattaaaggc tccttttgga gccctttttt ttggagattt tcaacgtgaa
     2521 aaaattatta ttcgcaattc ctttagttgt tcctttctat tctcactccg ctgaaactgt
     2581 tgaaagttgt ttagcaaaac ctcatacaga aaattcattt actaacgtct ggaaagacga
     2641 caaaacttta gactatttta ttcaagtact gttaacttta aaggcgcgcc cttaagaacg
     2701 ttactagtct cgagttataa caattgacgc gtaatattca tatgggtcct cctgtggagt
     2761 ggttctgtgg atcctaccaa ccggcacgat tgtgcccaca acagcatcgc ggtgccacgt
     2821 gtggaccgcg tcggtcagat cctccccgca cctctcgcca gccgtcaaga tcgaccgcgt
     2881 gcacctgcga tcgccgatca accgcgacta gcatcgggcg caagccgcca ctcgaacgga
     2941 cactcgatag cctaactaac gatttcaagg ccaaaaaagg ggccgaaccg tccgccggct
     3001 cgacccctcc cgctgtgcgc tacagcgccg caagctcccg ctcagtggct tcgctcgctt
     3061 cgtcttcctc cttcagcagc tccgcccact tgagcgtcac acggtccttc agggggatgt
     3121 atttcttcgt ctcagggtgg cgcccccggt cgaccatgac ggagtcaagg aagaagctca
     3181 ggaactcccg acgctcgtac acgtcccagc ttgcccagat gcccccttcg gccgtcgggt
     3241 cttcgccgct gaaccactca gacggaacgc gggtgctgcc gttcatcttc tcgtcaagct
     3301 cagcgagtcg ggtcgtgcac tccgcttcgt aggaccggta ttgcagcacc gttgagcgcc
     3361 acgtttccag ctcttcacgc ccgacgtaca gaccggcctt acggtccgcc tgaaggtcct
     3421 tgatggagcg ccgcacgttg tctaggtgcg cctgttgttc gcgccgctca tcggccaccc
     3481 ccgctaggtc gtgctgaagg gcgaagcgct ccgcagcggc ggcaatccat gcctgatcgt
     3541 gttcatcttc catgtcggct gtgcggagcc gtgcccacac cttcgaagca acgaattcgt
     3601 ccagttcgct gcgcttgacc gacaagccgc cgtgcccctt cgggttggcg cacatgtaat
     3661 tgccttcggc aaggtcgccg ttgcgcttac ggccaccctg ggactgaccc attgagccgc
     3721 cgcagattcg gcaccccagg aaacgccacc cgctcagaag cgtcggttcg acttcggccc
     3781 caggcttccg gtcgctgaga ttcttcccgg aacgcttctc ttgaagctca agccacttcg
     3841 agccgctgag aatgcccgtg tggggcgtta gcggcttgcc gccggggtcg cgccgtatga
     3901 cgttgatgtg cgccttaccg tgcttcacac gctcgaatgc gaaaccgccg attgcgggat
     3961 ggttgagaat ccatcggacc gtttgagcgc gccacatgat cggcttttca gcgccgttca
     4021 ggcgacgtgc cttgatggac gcaagacgct tttccgtggc gcgtctctca gccattccgg
     4081 gcgacgggat cttttccttc tcgaaggtcg ttgcaatggc gttgtcggac acgccttcga
     4141 acgacatttt cgccatgcgc tcaactagct cgacgtgatc cgggttgtct tcgtccggct
     4201 caagaacgga gatcacgaga ttatcgacct tcttgcgcac ggcgcgcatt ccgaacgggg
     4261 cggaagacga gtgaacgcca cccagcgcgg caatctcgtc tttcgcaccc ttcaggcgct
     4321 ccgccttcag gtcactgtcc tgtttcgcaa gggcagcgat cagcgcgaaa atggcgacgc
     4381 cgataggggt agacgtgtca aggaacggct caagaaccga catgaagcgc acgccgtgct
     4441 tcttcaattc gttgtcgatt tcgagcgcgt catgggcgcc cttgcgagtg agccgggaaa
     4501 gctcattcac aacgacaacg tcaccttcgc cggcgcggac ttcgcccatc atgcgctcga
     4561 agtcggcacg ggtcacgttc gggtcccagc cggagcgccc gacgtccacg aactcacctg
     4621 ccacgaccca gcgcgcaccc ccctgggcgt tgcgagacgc gaccaacgca cgaccggccg
     4681 ctagctgtgc ttcggtcgac acgtctgagc cgtcggaccg gcccttagac tgacgcgcgt
     4741 acaggaagac gcgaacagtg tctagaaggt gctcagggac gtcgggagcg atgaagggcg
     4801 acatgcctag atcttggctc atgggcgctg acctgcgctt tcttttggga acattggtgg
     4861 tgctgagtag tttcccatgg atcactgtcc agagacaaca acccagcacc cgaaacgtct
     4921 gaagccccgt ccggcgccag catgcctgca ggtcgacgga tcttttccgc tgcataaccc
     4981 tgcttcgggg tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca
     5041 ggattttgcc aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc
     5101 aggataggtg aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg
     5161 cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta
     5221 acagatgagg gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct
     5281 atcaaggtgt actgccttcc agacgaacga agagcgattg aggaaaaggc ggcggcggcc
     5341 ggcatgagcc tgtcggccta cctgctggcc gtcggccagg gctacaaaat cacgggcgtc
     5401 gtggactatg agcacgtccg cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc
     5461 ggcctgctga aactctggct caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg
     5521 atcctcgccc tgctggcgaa gatcgaagag aagcaggacg agcttggcaa ggtcatgatg
     5581 ggcgtggtcc gcccgagggc agagccatga cttttttagc cgctaaaacg gccggggggt
     5641 gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag aagagcgact tcgcggagct
     5701 ggtgaagtac atcaccgacg agcaaggcaa gaccgatccc cggggacctg caggtcgact
     5761 ctagctagct ggccagggcc cgatacgtcg cggtgagttc aggctttttc atatctcatt
     5821 gcccccggac gagcgtctgc tccgccattc gccgtccgcc gtgccaatcg gatcagccgt
     5881 ccaaatgcgg gattttcgtt agtcggaggc caaacggcat tgagcgtcag catatcatca
     5941 gcgagctgaa gaaagacaat ccccgatccg ctccacgtgt tgccccagca atcagcgcga
     6001 ccttgcccct ccaacgtcat ctcgttctcc gctcatgaga actgagaagc cccgtcaggc
     6061 accgcccgac ggggctcttc acccgggttg ccgagctggg atggaagctc ggccgaccac
     6121 cctggaggag atgatcgagg atgccagggc ctttcacgcc cgccgctgct gagcgtccgc
     6181 cgccgggccc gcaccgccgt cggccggccc gctccgggct cgcagcagcg ggcttcggcg
     6241 cgggcccggg gctcccgagc gcgggcgggg ctccgggcgg ccgccggggg ccgggggcgg
     6301 cgccgggcgg cccggggcgt caggcgccgg gggcggtgtc cggcggcccc cagaggaact
     6361 gcgccagttc ctccggatcg gtgaagccgg agagatccag cggggtctcc tcgaacacct
     6421 cgaagtcgtg caggaaggtg aaggcgagca gttcgcgggc gaagtcctcg gtccgcttcc
     6481 actgcgcccc gtcgagcagc gcggccagga tctcgcggtc gccccggaag gcgttgagat
     6541 gcagttgcac caggctgtag cgggagtctc ccgcatagac gtcggtgaag tcgacgatcc
     6601 cggtgacctc ggtcgcggcc aggtccacga agatgttggt cccgtgcagg tcgccgtgga
     6661 cgaaccgggg ttcgcggccg gccagcagcg tgtccacgtc cggcagccag tcctccaggc
     6721 ggtccagcag ccggggcgag aggtagcccc acccgcggtg gtcctcgacg gtcgccgcgc
     6781 ggcgttcccg cagcagttcc gggaagacct cggaatgggg ggtgagcacg gtgttcccgg
     6841 tcagcggcac cctgtgcagc cggccgagca cccggccgag ttcgcgggcc agggcgagca
     6901 gcgcgttccg gtcggtcgtg ccgtccatcg cggaccgcca ggtggtgccg gtcatccggc
     6961 tcatcaccag gtagggccac ggccaggctc cggtgccggg ccgcagctcg ccgcggccga
     7021 ggaggcgggg caccggcacc ggggcgtccg ccaggaccgc gtacgcctcc gactccgacg
     7081 cgaggctctc cggaccgcac cagtgctcgc cgaacagctt gatcaccggg tcgggctcgc
     7141 cgaccagtac ggggttggtg ctctcgccgg gcacccgcag caccggcggc accggcagcc
     7201 cgagctcctc cagggctcgg cgggccagcg gctcccagaa ttcctggtcg ttccgcaggc
     7261 tcgcgtagga atcatccgaa tcaatacggt cgagaagtaa cagggattct tgtgtcacag
     7321 cggacctcta ttcacagggt acgggccggc ttaattccgc acggccggtc gcgacacggc
     7381 ctgtccgcac cgcggatcag gcgttgacga tgacgggctg gtcggccacg tcggggacgt
     7441 tctcggtggt gctgcggtcg ggatcgccaa tctctacggg ccgaccgagg cgacggtgta
     7501 cgccaccgcc tggttctgcg acggcgaggc gccgtccagg ccccgcccga tccccgtccc
     7561 ccgcgtcgtc gagcgcggtg ccgacgacac cgccgcgtgg ctcgtcacgg aggccgtccc
     7621 cggcgtcgcg gcggccgagg agtggcccga gcaccagcgg ttcgccgtgg tcgaggcgat
     7681 ggcggagctg gcccgcgccc tccacgagct gcccgtggag gactgcccct tcgaccggcg
     7741 cctcgacgcg gcggtcgccg aggcccggcg gaacgtcgcc gagggcctgt ggacctcgac
     7801 gaccgatctg atgtgctcag tatcaccgcc agtggtattt atgtcaacac cgccagagat
     7861 aatttatcac cgcagatggt tacctcgcct ctgacccctg accccgtcag cctgaggttg
     7921 aaaaacgctc actggtacca tgcatgggga tgacgaaggt gatgccggac ccggcgaaga
     7981 aaccgacgcg ggatgccggt cctcaggcct gacgggacgc cagctcgatc accgtgatgt
     8041 ccgagggcgc ccccacgcgc gtgggcgggc cccaggcgcc cgcgccgcgg ctgacgtaga
     8101 gctgggtgtc gccgtagcgc tccaggcccg ccagcgtcgg attggcggcg cccgcgatga
     8161 ggttgccggg ccagagctgg ccgccgtggg tgtggccgga gagctggagg tcgactccgt
     8221 ggtccacggc gtcgtggatc tgcaccggct ggtgggcgag gagcacgcag gcgcgggatc
     8281 ggtcccggtc gccgagggcc ctgtcgtagt cggggccctg gccctcgtcc tcgccggcga
     8341 cgtcgttgac gccggccagg tcgaagtggg gcagctcggt gcgggcgttc tccagcggga
     8401 gcagcccgag ccgccgcacc tcggcgaccc actgctcggc gccggagaag tactcgtggt
     8461 tgccggtgac gaagtacgcg ccgtgccgtg ccgtcagccg ggccagcggg gcggcggccg
     8521 ggccgaggtc cttcacgctg ccgtccacca ggtcgccgac gacggcgatc aggtcgggct
     8581 gcgtggagtt gatcgtgtcg acgacctgct gcgcgaagcc ccggccgagc accgggccca
     8641 ggtggatgtc gctgacgacg gcgatgcgga agccgtgcgc cgcgcgcggg agcttggcca
     8701 gcggcaccgt gacccgcttc accttcgggc cgctcagcac gccgtacgtg ccgtagccga
     8761 ccgtgccgac ggccgccgcc gcggcggcac cggcgacgac gcgggagacg aagagacggc
     8821 gggagggtac ggcgaggggg ctgccctggg ctgccagggc ttcggtcccc ggtgcctcgg
     8881 gcgtctcagg tgcctcgggc gtcttcggcg gagccgtgcc ggccggtatg cgcgaggtgt
     8941 ccgcggccgg ggtgaccgag ggctccgccc ccgccgtccg ccgcgcggcc gccctgcgct
     9001 ccaggaaccg ccgcagcagc ggccggacga cctcgcccgc gagcaccgcg agcagcaggt
     9061 agatcgacag ggcgagccac aggaagcccg gccaggcgag cacccgctgg agccagaacg
     9121 gggcgcccgc gcgctcggcg accagcgccc cgaccgccag cagccagccc ccggcgatca
     9181 cggccgcgcc gattcggcgc acgggacccg gggcgcgggt ggtgtcgcgg aacagacgcc
     9241 gccacacgta ccagttggcc gtcaccagga cgcccaccat cagcaacgcg accagcacga
     9301 agacgaccac catgccgtcg tatcccctcg gttgggacgc tcagggcgtc cgtgacgtcc
     9361 tcgtcgcggt ttcagtatcg ccgcacgctg tccggatccg tgcgcggggt ggggaggggg
     9421 gtgggaccga cccgatcgcg gggggtacgt ttccgctcgt gcccgctccc aagaaggccc
     9481 tcaggcgatc cctgacggtc acctccgctg ccctgaccgc tctcgccctc ctctcccccg
     9541 ccgcgctcgc cgccccgagc ccgtcgacga gtccgtcggc cactccgccg gcgtcgatgt
     9601 cgggcgtggg cggtgcgcgg ctcgggaagc ccggaacgca ggtggacctg gcgggcgggg
     9661 cgcccgtgct gccgaaggac ctcacggccc ggtcgtggat cgtcgcggac gccgagtcgg
     9721 gcgacgtgct ggccgcgcac aacgcgcact gggagctggc cccggcgagc accctgaaga
     9781 tgctgttcgc a
//
