LOCUS       V004331                 9225 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004331
VERSION     V004331
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Pisciotta
            A, Alduina R, Manteca A.
  TITLE     New PhiBT1 site-specific integrative vectors with neutral phenotype
            in Streptomyces
  JOURNAL   Appl. Microbiol. Biotechnol. 100 (6), 2797-2808 (2016)
   PUBMED   26758297
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Manteca
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2015) Biologia Funcional, Universidad de Oviedo,
            c/Julian Claveria s/n Facultad de Medicina, Oviedo 33006, Spain
REFERENCE   3  (bases 1 to 9225)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2016"; volume: "100"; issue: "6";
            pages: "2797-2808"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-APR-2015) Biologia Funcional, Universidad de Oviedo, c/Julian
            Claveria s/n Facultad de Medicina, Oviedo 33006, Spain"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      265..853
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1141..1162
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1177..1207
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1215..1231
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1239..1255
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     oriT            3480..3589
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             3622..3990
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(4706..5701)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     CDS             complement(6386..7696)
                     /codon_start=1
                     /gene="SCO4849"
                     /product="SCO4849 protein"
                     /label="SCO4849"
                     /protein_id="AML61765.1"
                     /translation="MVVVFVLVALLMVGVLVTANWYVWRRLFRDTTRAPGPVRRIGAAV
                     IAGGWLLAVGALVAERAGAPFWLQRVLAWPGFLWLALSIYLLLAVLAGEVVRPLLRRFL
                     ERRAAARRTAGAEPSVTPAADTSRIPAGTAPPKTPEAPETPEAPGTEALAAQGSPLAVP
                     SRRLFVSRVVAGAAAAAAVGTVGYGTYGVLSGPKVKRVTVPLAKLPRAAHGFRIAVVSD
                     IHLGPVLGRGFAQQVVDTINSTQPDLIAVVGDLVDGSVKDLGPAAAPLARLTARHGAYF
                     VTGNHEYFSGAEQWVAEVRRLGLLPLENARTELPHFDLAGVNDVAGEDEGQGPDYDRAL
                     GDRDRSRACVLLAHQPVQIHDAVDHGVDLQLSGHTHGGQLWPGNLIAGAANPTLAGLER
                     YGDTQLYVSRGAGAWGPPTRVGAPSDITVIELASRQA"
     gene            complement(6386..7696)
                     /gene="SCO4849"
                     /label="SCO4849"
     regulatory      complement(7757..8179)
                     /regulatory_class="promoter"
     CDS             complement(8300..9157)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9158..9221)
                     /label="AmpR promoter"
ORIGIN
        1 ctaggttctc ggcttttcgc cattcgtatt gcacgacatt gcactccacc gctgatgaca
       61 tcagtcgatc atagcacgat caacggcact gttgcaaata gtcggtggtg ataaacttat
      121 catccccttt tgctgatgga gctgcacatg aaccaaaagg atctaggtga agatcctttt
      181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      901 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      961 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1021 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1081 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1141 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1201 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     1261 tacgaattcg atatcgcgcg cggccgcgga tccgctccct gcccgctatg gtgacgaagg
     1321 aactactagt tagcctaact aacgatttca aggccaaaaa aggggccgaa ccgtccgccg
     1381 gctcgacccc tcccgctgtg cgctacagcg ccgcaagctc ccgctcagtg gcttcgctcg
     1441 cttcgtcttc ctccttcagc agctccgccc acttgagcgt cacacggtcc ttcaggggga
     1501 tgtatttctt cgtctcaggg tggcgccccc ggtcgaccat gacggagtca aggaagaagc
     1561 tcaggaactc ccgacgctcg tacacgtccc agcttgccca gatgccccct tcggccgtcg
     1621 ggtcttcgcc gctgaaccac tcagacggaa cgcgggtgct gccgttcatc ttctcgtcaa
     1681 gctcagcgag tcgggtcgtg cactccgctt cgtaggaccg gtattgcagc accgttgagc
     1741 gccacgtttc cagctcttca cgcccgacgt acagaccggc cttacggtcc gcctgaaggt
     1801 ccttgatgga gcgccgcacg ttgtctaggt gcgcctgttg ttcgcgccgc tcatcggcca
     1861 cccccgctag gtcgtgctga agggcgaagc gctccgcagc ggcggcaatc catgcctgat
     1921 cgtgttcatc ttccatgtcg gctgtgcgga gccgtgccca caccttcgaa gcaacgaatt
     1981 cgtccagttc gctgcgcttg accgacaagc cgccgtgccc cttcgggttg gcgcacatgt
     2041 aattgccttc ggcaaggtcg ccgttgcgct tacggccacc ctgggactga cccattgagc
     2101 cgccgcagat tcggcacccc aggaaacgcc acccgctcag aagcgtcggt tcgacttcgg
     2161 ccccaggctt ccggtcgctg agattcttcc cggaacgctt ctcttgaagc tcaagccact
     2221 tcgagccgct gagaatgccc gtgtggggcg ttagcggctt gccgccgggg tcgcgccgta
     2281 tgacgttgat gtgcgcctta ccgtgcttca cacgctcgaa tgcgaaaccg ccgattgcgg
     2341 gatggttgag aatccatcgg accgtttgag cgcgccacat gatcggcttt tcagcgccgt
     2401 tcaggcgacg tgccttgatg gacgcaagac gcttttccgt ggcgcgtctc tcagccattc
     2461 cgggcgacgg gatcttttcc ttctcgaagg tcgttgcaat ggcgttgtcg gacacgcctt
     2521 cgaacgacat tttcgccatg cgctcaacta gctcgacgtg atccgggttg tcttcgtccg
     2581 gctcaagaac ggagatcacg agattatcga ccttcttgcg cacggcgcgc attccgaacg
     2641 gggcggaaga cgagtgaacg ccacccagcg cggcaatctc gtctttcgca cccttcaggc
     2701 gctccgcctt caggtcactg tcctgtttcg caagggcagc gatcagcgcg aaaatggcga
     2761 cgccgatagg ggtagacgtg tcaaggaacg gctcaagaac cgacatgaag cgcacgccgt
     2821 gcttcttcaa ttcgttgtcg atttcgagcg cgtcatgggc gcccttgcga gtgagccggg
     2881 aaagctcatt cacaacgaca acgtcacctt cgccggcgcg gacttcgccc atcatgcgct
     2941 cgaagtcggc acgggtcacg ttcgggtccc agccggagcg cccgacgtcc acgaactcac
     3001 ctgccacgac ccagcgcgca cccccctggg cgttgcgaga cgcgaccaac gcacgaccgg
     3061 ccgctagctg tgcttcggtc gacacgtctg agccgtcgga ccggccctta gactgacgcg
     3121 cgtacaggaa gacgcgaaca gtgtctagaa ggtgctcagg gacgtcggga gcgatgaagg
     3181 gcgacatgcc tagatcttgg ctcatgggcg ctgacctgcg ctttcttttg ggaacattgg
     3241 tggtgctgag tagtttccca tggatcactg tccagagaca acaacccagc acccgaaacg
     3301 tctgaagccc cgtccggcgc cagcatgcct gcaggtcgac ggatcttttc cgctgcataa
     3361 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
     3421 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
     3481 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
     3541 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
     3601 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
     3661 cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg
     3721 gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc
     3781 gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg
     3841 ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc
     3901 acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg
     3961 atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg
     4021 ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga
     4081 gctggtgaag tacatcaccg acgagcaagg caagaccgat ccccggggac ctgcaggtcg
     4141 actctagcta gctggccagg gcccgatacg tcgcggtgag ttcaggcttt ttcatatctc
     4201 attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa tcggatcagc
     4261 cgtccaaatg cgggattttc gttagtcgga ggccaaacgg cattgagcgt cagcatatca
     4321 tcagcgagct gaagaaagac aatccccgat ccgctccacg tgttgcccca gcaatcagcg
     4381 cgaccttgcc cctccaacgt catctcgttc tccgctcatg agaactgaga agccccgtca
     4441 ggcaccgccc gacggggctc ttcacccggg ttgccgagct gggatggaag ctcggccgac
     4501 caccctggag gagatgatcg aggatgccag ggcctttcac gcccgccgct gctgagcgtc
     4561 cgccgccggg cccgcaccgc cgtcggccgg cccgctccgg gctcgcagca gcgggcttcg
     4621 gcgcgggccc ggggctcccg agcgcgggcg gggctccggg cggccgccgg gggccggggg
     4681 cggcgccggg cggcccgggg cgtcaggcgc cgggggcggt gtccggcggc ccccagagga
     4741 actgcgccag ttcctccgga tcggtgaagc cggagagatc cagcggggtc tcctcgaaca
     4801 cctcgaagtc gtgcaggaag gtgaaggcga gcagttcgcg ggcgaagtcc tcggtccgct
     4861 tccactgcgc cccgtcgagc agcgcggcca ggatctcgcg gtcgccccgg aaggcgttga
     4921 gatgcagttg caccaggctg tagcgggagt ctcccgcata gacgtcggtg aagtcgacga
     4981 tcccggtgac ctcggtcgcg gccaggtcca cgaagatgtt ggtcccgtgc aggtcgccgt
     5041 ggacgaaccg gggttcgcgg ccggccagca gcgtgtccac gtccggcagc cagtcctcca
     5101 ggcggtccag cagccggggc gagaggtagc cccacccgcg gtggtcctcg acggtcgccg
     5161 cgcggcgttc ccgcagcagt tccgggaaga cctcggaatg gggggtgagc acggtgttcc
     5221 cggtcagcgg caccctgtgc agccggccga gcacccggcc gagttcgcgg gccagggcga
     5281 gcagcgcgtt ccggtcggtc gtgccgtcca tcgcggaccg ccaggtggtg ccggtcatcc
     5341 ggctcatcac caggtagggc cacggccagg ctccggtgcc gggccgcagc tcgccgcggc
     5401 cgaggaggcg gggcaccggc accggggcgt ccgccaggac cgcgtacgcc tccgactccg
     5461 acgcgaggct ctccggaccg caccagtgct cgccgaacag cttgatcacc gggtcgggct
     5521 cgccgaccag tacggggttg gtgctctcgc cgggcacccg cagcaccggc ggcaccggca
     5581 gcccgagctc ctccagggct cggcgggcca gcggctccca gaattcctgg tcgttccgca
     5641 ggctcgcgta ggaatcatcc gaatcaatac ggtcgagaag taacagggat tcttgtgtca
     5701 cagcggacct ctattcacag ggtacgggcc ggcttaattc cgcacggccg gtcgcgacac
     5761 ggcctgtccg caccgcggat caggcgttga cgatgacggg ctggtcggcc acgtcgggga
     5821 cgttctcggt ggtgctgcgg tcgggatcgc caatctctac gggccgaccg aggcgacggt
     5881 gtacgccacc gcctggttct gcgacggcga ggcgccgtcc aggccccgcc cgatccccgt
     5941 cccccgcgtc gtcgagcgcg gtgccgacga caccgccgcg tggctcgtca cggaggccgt
     6001 ccccggcgtc gcggcggccg aggagtggcc cgagcaccag cggttcgccg tggtcgaggc
     6061 gatggcggag ctggcccgcg ccctccacga gctgcccgtg gaggactgcc ccttcgaccg
     6121 gcgcctcgac gcggcggtcg ccgaggcccg gcggaacgtc gccgagggcc tgtggacctc
     6181 gacgaccgat ctgatgtgct cagtatcacc gccagtggta tttatgtcaa caccgccaga
     6241 gataatttat caccgcagat ggttacctcg cctctgaccc ctgaccccgt cagcctgagg
     6301 ttgaaaaacg ctcactggta ccatgcatgg ggatgacgaa ggtgatgccg gacccggcga
     6361 agaaaccgac gcgggatgcc ggtcctcagg cctgacggga cgccagctcg atcaccgtga
     6421 tgtccgaggg cgcccccacg cgcgtgggcg ggccccaggc gcccgcgccg cggctgacgt
     6481 agagctgggt gtcgccgtag cgctccaggc ccgccagcgt cggattggcg gcgcccgcga
     6541 tgaggttgcc gggccagagc tggccgccgt gggtgtggcc ggagagctgg aggtcgactc
     6601 cgtggtccac ggcgtcgtgg atctgcaccg gctggtgggc gaggagcacg caggcgcggg
     6661 atcggtcccg gtcgccgagg gccctgtcgt agtcggggcc ctggccctcg tcctcgccgg
     6721 cgacgtcgtt gacgccggcc aggtcgaagt ggggcagctc ggtgcgggcg ttctccagcg
     6781 ggagcagccc gagccgccgc acctcggcga cccactgctc ggcgccggag aagtactcgt
     6841 ggttgccggt gacgaagtac gcgccgtgcc gtgccgtcag ccgggccagc ggggcggcgg
     6901 ccgggccgag gtccttcacg ctgccgtcca ccaggtcgcc gacgacggcg atcaggtcgg
     6961 gctgcgtgga gttgatcgtg tcgacgacct gctgcgcgaa gccccggccg agcaccgggc
     7021 ccaggtggat gtcgctgacg acggcgatgc ggaagccgtg cgccgcgcgc gggagcttgg
     7081 ccagcggcac cgtgacccgc ttcaccttcg ggccgctcag cacgccgtac gtgccgtagc
     7141 cgaccgtgcc gacggccgcc gccgcggcgg caccggcgac gacgcgggag acgaagagac
     7201 ggcgggaggg tacggcgagg gggctgccct gggctgccag ggcttcggtc cccggtgcct
     7261 cgggcgtctc aggtgcctcg ggcgtcttcg gcggagccgt gccggccggt atgcgcgagg
     7321 tgtccgcggc cggggtgacc gagggctccg cccccgccgt ccgccgcgcg gccgccctgc
     7381 gctccaggaa ccgccgcagc agcggccgga cgacctcgcc cgcgagcacc gcgagcagca
     7441 ggtagatcga cagggcgagc cacaggaagc ccggccaggc gagcacccgc tggagccaga
     7501 acggggcgcc cgcgcgctcg gcgaccagcg ccccgaccgc cagcagccag cccccggcga
     7561 tcacggccgc gccgattcgg cgcacgggac ccggggcgcg ggtggtgtcg cggaacagac
     7621 gccgccacac gtaccagttg gccgtcacca ggacgcccac catcagcaac gcgaccagca
     7681 cgaagacgac caccatgccg tcgtatcccc tcggttggga cgctcagggc gtccgtgacg
     7741 tcctcgtcgc catatgggtt tcagtatcgc cgcacgctgt ccggatccgt gcgcggggtg
     7801 gggagggggg tgggaccgac ccgatcgcgg ggggtacgtt tccgctcgtg cccgctccca
     7861 agaaggccct caggcgatcc ctgacggtca cctccgctgc cctgaccgct ctcgccctcc
     7921 tctcccccgc cgcgctcgcc gccccgagcc cgtcgacgag tccgtcggcc actccgccgg
     7981 cgtcgatgtc gggcgtgggc ggtgcgcggc tcgggaagcc cggaacgcag gtggacctgg
     8041 cgggcggggc gcccgtgctg ccgaaggacc tcacggcccg gtcgtggatc gtcgcggacg
     8101 ccgagtcggg cgacgtgctg gccgcgcaca acgcgcactg ggagctggcc ccggcgagca
     8161 ccctgaagat gctgttcgca agcttaggga ttttggtcat gagattatca aaaaggatct
     8221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8581 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     8641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8881 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     8941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9181 gaagcattta tcagggttat tgtctcatga gcggatacat atttc
//
