LOCUS       V004332                 8756 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004332
VERSION     V004332
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Pisciotta
            A, Alduina R, Manteca A.
  TITLE     New PhiBT1 site-specific integrative vectors with neutral phenotype
            in Streptomyces
  JOURNAL   Appl. Microbiol. Biotechnol. 100 (6), 2797-2808 (2016)
   PUBMED   26758297
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Manteca
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2015) Biologia Funcional, Universidad de Oviedo,
            c/Julian Claveria s/n Facultad de Medicina, Oviedo 33006, Spain
REFERENCE   3  (bases 1 to 8756)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2016"; volume: "100"; issue: "6";
            pages: "2797-2808"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-APR-2015) Biologia Funcional, Universidad de Oviedo, c/Julian
            Claveria s/n Facultad de Medicina, Oviedo 33006, Spain"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            2150..2259
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             2292..2660
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(3376..4371)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     CDS             complement(5056..6366)
                     /codon_start=1
                     /gene="SCO4849"
                     /product="SCO4849 protein"
                     /label="SCO4849"
                     /protein_id="AML61763.1"
                     /translation="MVVVFVLVALLMVGVLVTANWYVWRRLFRDTTRAPGPVRRIGAAV
                     IAGGWLLAVGALVAERAGAPFWLQRVLAWPGFLWLALSIYLLLAVLAGEVVRPLLRRFL
                     ERRAAARRTAGAEPSVTPAADTSRIPAGTAPPKTPEAPETPEAPGTEALAAQGSPLAVP
                     SRRLFVSRVVAGAAAAAAVGTVGYGTYGVLSGPKVKRVTVPLAKLPRAAHGFRIAVVSD
                     IHLGPVLGRGFAQQVVDTINSTQPDLIAVVGDLVDGSVKDLGPAAAPLARLTARHGAYF
                     VTGNHEYFSGAEQWVAEVRRLGLLPLENARTELPHFDLAGVNDVAGEDEGQGPDYDRAL
                     GDRDRSRACVLLAHQPVQIHDAVDHGVDLQLSGHTHGGQLWPGNLIAGAANPTLAGLER
                     YGDTQLYVSRGAGAWGPPTRVGAPSDITVIELASRQA"
     gene            complement(5056..6366)
                     /gene="SCO4849"
                     /label="SCO4849"
     regulatory      complement(6421..6843)
                     /regulatory_class="promoter"
     rep_origin      7119..7707
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7995..8016
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8031..8061
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8069..8085
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8093..8109
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      8272..8320
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      complement(8631..8634)
                     /regulatory_class="ribosome_binding_site"
ORIGIN
        1 tagcctaact aacgatttca aggccaaaaa aggggccgaa ccgtccgccg gctcgacccc
       61 tcccgctgtg cgctacagcg ccgcaagctc ccgctcagtg gcttcgctcg cttcgtcttc
      121 ctccttcagc agctccgccc acttgagcgt cacacggtcc ttcaggggga tgtatttctt
      181 cgtctcaggg tggcgccccc ggtcgaccat gacggagtca aggaagaagc tcaggaactc
      241 ccgacgctcg tacacgtccc agcttgccca gatgccccct tcggccgtcg ggtcttcgcc
      301 gctgaaccac tcagacggaa cgcgggtgct gccgttcatc ttctcgtcaa gctcagcgag
      361 tcgggtcgtg cactccgctt cgtaggaccg gtattgcagc accgttgagc gccacgtttc
      421 cagctcttca cgcccgacgt acagaccggc cttacggtcc gcctgaaggt ccttgatgga
      481 gcgccgcacg ttgtctaggt gcgcctgttg ttcgcgccgc tcatcggcca cccccgctag
      541 gtcgtgctga agggcgaagc gctccgcagc ggcggcaatc catgcctgat cgtgttcatc
      601 ttccatgtcg gctgtgcgga gccgtgccca caccttcgaa gcaacgaatt cgtccagttc
      661 gctgcgcttg accgacaagc cgccgtgccc cttcgggttg gcgcacatgt aattgccttc
      721 ggcaaggtcg ccgttgcgct tacggccacc ctgggactga cccattgagc cgccgcagat
      781 tcggcacccc aggaaacgcc acccgctcag aagcgtcggt tcgacttcgg ccccaggctt
      841 ccggtcgctg agattcttcc cggaacgctt ctcttgaagc tcaagccact tcgagccgct
      901 gagaatgccc gtgtggggcg ttagcggctt gccgccgggg tcgcgccgta tgacgttgat
      961 gtgcgcctta ccgtgcttca cacgctcgaa tgcgaaaccg ccgattgcgg gatggttgag
     1021 aatccatcgg accgtttgag cgcgccacat gatcggcttt tcagcgccgt tcaggcgacg
     1081 tgccttgatg gacgcaagac gcttttccgt ggcgcgtctc tcagccattc cgggcgacgg
     1141 gatcttttcc ttctcgaagg tcgttgcaat ggcgttgtcg gacacgcctt cgaacgacat
     1201 tttcgccatg cgctcaacta gctcgacgtg atccgggttg tcttcgtccg gctcaagaac
     1261 ggagatcacg agattatcga ccttcttgcg cacggcgcgc attccgaacg gggcggaaga
     1321 cgagtgaacg ccacccagcg cggcaatctc gtctttcgca cccttcaggc gctccgcctt
     1381 caggtcactg tcctgtttcg caagggcagc gatcagcgcg aaaatggcga cgccgatagg
     1441 ggtagacgtg tcaaggaacg gctcaagaac cgacatgaag cgcacgccgt gcttcttcaa
     1501 ttcgttgtcg atttcgagcg cgtcatgggc gcccttgcga gtgagccggg aaagctcatt
     1561 cacaacgaca acgtcacctt cgccggcgcg gacttcgccc atcatgcgct cgaagtcggc
     1621 acgggtcacg ttcgggtccc agccggagcg cccgacgtcc acgaactcac ctgccacgac
     1681 ccagcgcgca cccccctggg cgttgcgaga cgcgaccaac gcacgaccgg ccgctagctg
     1741 tgcttcggtc gacacgtctg agccgtcgga ccggccctta gactgacgcg cgtacaggaa
     1801 gacgcgaaca gtgtctagaa ggtgctcagg gacgtcggga gcgatgaagg gcgacatgcc
     1861 tagatcttgg ctcatgggcg ctgacctgcg ctttcttttg ggaacattgg tggtgctgag
     1921 tagtttccca tggatcactg tccagagaca acaacccagc acccgaaacg tctgaagccc
     1981 cgtccggcgc cagcatgcct gcaggtcgac ggatcttttc cgctgcataa ccctgcttcg
     2041 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     2101 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     2161 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
     2221 cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc gtaacagatg
     2281 agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca cctatcaagg
     2341 tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa ggcggcggcg gccggcatga
     2401 gcctgtcggc ctacctgctg gccgtcggcc agggctacaa aatcacgggc gtcgtggact
     2461 atgagcacgt ccgcgagctg gcccgcatca atggcgacct gggccgcctg ggcggcctgc
     2521 tgaaactctg gctcaccgac gacccgcgca cggcgcggtt cggtgatgcc acgatcctcg
     2581 ccctgctggc gaagatcgaa gagaagcagg acgagcttgg caaggtcatg atgggcgtgg
     2641 tccgcccgag ggcagagcca tgactttttt agccgctaaa acggccgggg ggtgcgcgtg
     2701 attgccaagc acgtccccat gcgctccatc aagaagagcg acttcgcgga gctggtgaag
     2761 tacatcaccg acgagcaagg caagaccgat ccccggggac ctgcaggtcg actctagcta
     2821 gctggccagg gcccgatacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccg
     2881 gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa tcggatcagc cgtccaaatg
     2941 cgggattttc gttagtcgga ggccaaacgg cattgagcgt cagcatatca tcagcgagct
     3001 gaagaaagac aatccccgat ccgctccacg tgttgcccca gcaatcagcg cgaccttgcc
     3061 cctccaacgt catctcgttc tccgctcatg agaactgaga agccccgtca ggcaccgccc
     3121 gacggggctc ttcacccggg ttgccgagct gggatggaag ctcggccgac caccctggag
     3181 gagatgatcg aggatgccag ggcctttcac gcccgccgct gctgagcgtc cgccgccggg
     3241 cccgcaccgc cgtcggccgg cccgctccgg gctcgcagca gcgggcttcg gcgcgggccc
     3301 ggggctcccg agcgcgggcg gggctccggg cggccgccgg gggccggggg cggcgccggg
     3361 cggcccgggg cgtcaggcgc cgggggcggt gtccggcggc ccccagagga actgcgccag
     3421 ttcctccgga tcggtgaagc cggagagatc cagcggggtc tcctcgaaca cctcgaagtc
     3481 gtgcaggaag gtgaaggcga gcagttcgcg ggcgaagtcc tcggtccgct tccactgcgc
     3541 cccgtcgagc agcgcggcca ggatctcgcg gtcgccccgg aaggcgttga gatgcagttg
     3601 caccaggctg tagcgggagt ctcccgcata gacgtcggtg aagtcgacga tcccggtgac
     3661 ctcggtcgcg gccaggtcca cgaagatgtt ggtcccgtgc aggtcgccgt ggacgaaccg
     3721 gggttcgcgg ccggccagca gcgtgtccac gtccggcagc cagtcctcca ggcggtccag
     3781 cagccggggc gagaggtagc cccacccgcg gtggtcctcg acggtcgccg cgcggcgttc
     3841 ccgcagcagt tccgggaaga cctcggaatg gggggtgagc acggtgttcc cggtcagcgg
     3901 caccctgtgc agccggccga gcacccggcc gagttcgcgg gccagggcga gcagcgcgtt
     3961 ccggtcggtc gtgccgtcca tcgcggaccg ccaggtggtg ccggtcatcc ggctcatcac
     4021 caggtagggc cacggccagg ctccggtgcc gggccgcagc tcgccgcggc cgaggaggcg
     4081 gggcaccggc accggggcgt ccgccaggac cgcgtacgcc tccgactccg acgcgaggct
     4141 ctccggaccg caccagtgct cgccgaacag cttgatcacc gggtcgggct cgccgaccag
     4201 tacggggttg gtgctctcgc cgggcacccg cagcaccggc ggcaccggca gcccgagctc
     4261 ctccagggct cggcgggcca gcggctccca gaattcctgg tcgttccgca ggctcgcgta
     4321 ggaatcatcc gaatcaatac ggtcgagaag taacagggat tcttgtgtca cagcggacct
     4381 ctattcacag ggtacgggcc ggcttaattc cgcacggccg gtcgcgacac ggcctgtccg
     4441 caccgcggat caggcgttga cgatgacggg ctggtcggcc acgtcgggga cgttctcggt
     4501 ggtgctgcgg tcgggatcgc caatctctac gggccgaccg aggcgacggt gtacgccacc
     4561 gcctggttct gcgacggcga ggcgccgtcc aggccccgcc cgatccccgt cccccgcgtc
     4621 gtcgagcgcg gtgccgacga caccgccgcg tggctcgtca cggaggccgt ccccggcgtc
     4681 gcggcggccg aggagtggcc cgagcaccag cggttcgccg tggtcgaggc gatggcggag
     4741 ctggcccgcg ccctccacga gctgcccgtg gaggactgcc ccttcgaccg gcgcctcgac
     4801 gcggcggtcg ccgaggcccg gcggaacgtc gccgagggcc tgtggacctc gacgaccgat
     4861 ctgatgtgct cagtatcacc gccagtggta tttatgtcaa caccgccaga gataatttat
     4921 caccgcagat ggttacctcg cctctgaccc ctgaccccgt cagcctgagg ttgaaaaacg
     4981 ctcactggta ccatgcatgg ggatgacgaa ggtgatgccg gacccggcga agaaaccgac
     5041 gcgggatgcc ggtcctcagg cctgacggga cgccagctcg atcaccgtga tgtccgaggg
     5101 cgcccccacg cgcgtgggcg ggccccaggc gcccgcgccg cggctgacgt agagctgggt
     5161 gtcgccgtag cgctccaggc ccgccagcgt cggattggcg gcgcccgcga tgaggttgcc
     5221 gggccagagc tggccgccgt gggtgtggcc ggagagctgg aggtcgactc cgtggtccac
     5281 ggcgtcgtgg atctgcaccg gctggtgggc gaggagcacg caggcgcggg atcggtcccg
     5341 gtcgccgagg gccctgtcgt agtcggggcc ctggccctcg tcctcgccgg cgacgtcgtt
     5401 gacgccggcc aggtcgaagt ggggcagctc ggtgcgggcg ttctccagcg ggagcagccc
     5461 gagccgccgc acctcggcga cccactgctc ggcgccggag aagtactcgt ggttgccggt
     5521 gacgaagtac gcgccgtgcc gtgccgtcag ccgggccagc ggggcggcgg ccgggccgag
     5581 gtccttcacg ctgccgtcca ccaggtcgcc gacgacggcg atcaggtcgg gctgcgtgga
     5641 gttgatcgtg tcgacgacct gctgcgcgaa gccccggccg agcaccgggc ccaggtggat
     5701 gtcgctgacg acggcgatgc ggaagccgtg cgccgcgcgc gggagcttgg ccagcggcac
     5761 cgtgacccgc ttcaccttcg ggccgctcag cacgccgtac gtgccgtagc cgaccgtgcc
     5821 gacggccgcc gccgcggcgg caccggcgac gacgcgggag acgaagagac ggcgggaggg
     5881 tacggcgagg gggctgccct gggctgccag ggcttcggtc cccggtgcct cgggcgtctc
     5941 aggtgcctcg ggcgtcttcg gcggagccgt gccggccggt atgcgcgagg tgtccgcggc
     6001 cggggtgacc gagggctccg cccccgccgt ccgccgcgcg gccgccctgc gctccaggaa
     6061 ccgccgcagc agcggccgga cgacctcgcc cgcgagcacc gcgagcagca ggtagatcga
     6121 cagggcgagc cacaggaagc ccggccaggc gagcacccgc tggagccaga acggggcgcc
     6181 cgcgcgctcg gcgaccagcg ccccgaccgc cagcagccag cccccggcga tcacggccgc
     6241 gccgattcgg cgcacgggac ccggggcgcg ggtggtgtcg cggaacagac gccgccacac
     6301 gtaccagttg gccgtcacca ggacgcccac catcagcaac gcgaccagca cgaagacgac
     6361 caccatgccg tcgtatcccc tcggttggga cgctcagggc gtccgtgacg tcctcgtcgc
     6421 ggtttcagta tcgccgcacg ctgtccggat ccgtgcgcgg ggtggggagg ggggtgggac
     6481 cgacccgatc gcggggggta cgtttccgct cgtgcccgct cccaagaagg ccctcaggcg
     6541 atccctgacg gtcacctccg ctgccctgac cgctctcgcc ctcctctccc ccgccgcgct
     6601 cgccgccccg agcccgtcga cgagtccgtc ggccactccg ccggcgtcga tgtcgggcgt
     6661 gggcggtgcg cggctcggga agcccggaac gcaggtggac ctggcgggcg gggcgcccgt
     6721 gctgccgaag gacctcacgg cccggtcgtg gatcgtcgcg gacgccgagt cgggcgacgt
     6781 gctggccgcg cacaacgcgc actgggagct ggccccggcg agcaccctga agatgctgtt
     6841 cgcaagcttg gatcctaggt tctcggcttt tcgccattcg tattgcacga cattgcactc
     6901 caccgctgat gacatcagtc gatcatagca cgatcaacgg cactgttgca aatagtcggt
     6961 ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccaa aaggatctag
     7021 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7081 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7141 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7201 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7261 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7321 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7381 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7441 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7501 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7561 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7621 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7681 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7741 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7801 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7861 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7921 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7981 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8041 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8101 agctatgaca tgattacgaa ttcgatatct cccgcaaaag cggcctttga ctccctgcaa
     8161 gcctcagcga ccgaatatat cggttatgcg tgggcgatgg ttgttgtcat tgtcggcgca
     8221 actatcggta tcaagctgtt taagaaattc acctcgaaag caagctgata aaccgataca
     8281 attaaaggct ccttttggag cccttttttt tggagatttt caacgtgaaa aaattattat
     8341 tcgcaattcc tttagttgtt cctttctatt ctcactccgc tgaaactgtt gaaagttgtt
     8401 tagcaaaacc tcatacagaa aattcattta ctaacgtctg gaaagacgac aaaactttag
     8461 actattttat tcaagtactg ttaactttaa aggcgcgccc ttaagaacgt tactagtctc
     8521 gagttataac aattgacgcg taatattcat atgggtcctc ctgtggagtg gttctgtgga
     8581 tcctaccaac cggcacgatt gtgcccacaa cagcatcgcg gtgccacgtg tggaccgcgt
     8641 cggtcagatc ctccccgcac ctctcgccag ccgtcaagat cgaccgcgtg cacctgcgat
     8701 cgccgatcaa ccgcgactag catcgggcgc aagccgccac tcgaacggac actcga
//
