LOCUS       V004334                 8960 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004334
VERSION     V004334
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   DasSarma S.
  TITLE     Natural plasmids and plasmid vectors of halophiles
  JOURNAL   (in) DasSarma,S., Fleischmann,E.M., Robb,F.T., Place,A.R.,
            Sowers,K.R. and Schreier,H.J. (Eds.); ARCHAEA, A LABORATORY MANUAL -
            HALOPHILES: 241-250; Cold Spring Harbor Laboratory Press, Cold
            Spring Harbor, NY (1995)
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   DasSarma S.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2003) Center of Marine Biotechnology, University
            of Maryland Biotechnology Institute, 701 E. Pratt Street, Suite 236,
            Baltimore, MD 21202
REFERENCE   3  (bases 1 to 8960)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8960)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in)
            DasSarma,S., Fleischmann,E.M., Robb,F.T., Place,A.R., Sowers,K.R.
            and Schreier,H.J. (Eds.)"; volume: " ARCHAEA, A LABORATORY MANUAL -
            HALOPHILES"; pages: " 241-250; Cold Spring Harbor Laboratory Press,
            Cold Spring Harbor, NY (1995"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2003) Center of Marine Biotechnology, University of Maryland
            Biotechnology Institute, 701 E. Pratt Street, Suite 236, Baltimore,
            MD 21202"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             849..3878
                     /codon_start=1
                     /gene="repH"
                     /product="RepH"
                     /label="repH"
                     /note="replication protein; necessary for replication in
                     haloarchaea"
                     /protein_id="AAP44985.1"
                     /translation="MHTPNQQQGIRKIVPGGTLSTAGITITEVTPRVTEWIPDLLEELL
                     PRSIQSVRKFIRQEDPEVLTHARYNTVYRRLQEETLRFDHQEWCSTTDIWSDAEAEAVE
                     YVESLVEFAVKYSDVDEDDLDELSEYHQQRCKSLKQTLTTISTGRGPLNAGLEALAKGP
                     VRLHDELDDAPQPITLVLDGELWSKLDDRGTGIRALAAIAVLGSTFDVRLVISPALDAA
                     IERRYPDWYDSHLRLTETRETSSVESAGGDGQPSAEQLEEAWEAIQNLPEESGRLRLLR
                     NLPIEGSRDYRDLKQDDEIDVQAGTVGRYILDLEELGLVDIDRRGQYNSASLTGLGQVA
                     VEQYVTTDYRVIHPTQSTLETHLTPTPQPQASTVYPARSDTREGDQPGTAEDWIAATGS
                     PSEGADYVQWLDGPSGVLDAWGMHQRYLAGRRDRGVTLVDDRIERFEDGRVSYLSCFDD
                     DLFVATQWGGPLPTLGRIAGALLSDKALSKILTPSRLGNQFEEINDAVVEQLDREAGEI
                     IRRGHQIGWFSEDEEDYDGWRERIGSVRSLCLQQVGELTNSDDVEARTELLRDLHGLVA
                     SATQLYYAAGVDVTINVRVPDTGMLISDERRLDDFLGFARYTIPKQSVYGIHSGYRMLL
                     EDRPEKLKRRLPYEVDDADSTMHLTASWVFSGSTMIDLHDDIEDAIEMETNEIREAIAN
                     GQESAPVMEIPVQIGNSYSAIRNHVEDYASAKNYQVAHQEDIHEGKQDLERLVRLFLRV
                     LGTEDRPHRACPHDVAEAMLHVAQSSRNYDFITVRDISYGLSNLPTKRLLPELPPTATK
                     LLKTLLDADDPMGRSEIIDTADISESSYDRYINELAAWDIIEPREIEGHRRWEAHLEPW
                     WTPQSDRDEPYADPDPDTGILYAEFPRDVASAVMCHLITHYDLPDLETAYLEGIQPGDD
                     IKALFDDHDRLRRWRPFLWGAFADSDKLERGPSGTAASDSTVVRLGQSPGPDTAQSSFQ
                     DVSETATQRDRLSQPSPGLD"
     gene            849..3878
                     /gene="repH"
                     /label="repH"
     protein_bind    4431..4452
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4467..4497
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4505..4521
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4529..4545
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4566..4584
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(4597..4704)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(4713..4731)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4741..4757)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4899..5354
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5380..5484
                     /label="AmpR promoter"
     CDS             5485..6342
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6516..7104
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7428..8636)
                     /gene="hmgA"
                     /label="3-hydroxy-3-methylglutaryl-coenzyme A reductase"
                     /note="3-hydroxy-3-methylglutaryl-coenzyme A reductase from
                     Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879
                     / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2). Accession#:
                     Q59468"
ORIGIN
        1 gatccctaag tggacagaca tggctttgtc atcatttgga tgctcgtgaa ttgcattgag
       61 aatatgatag tgccgctttt tatgtagggg aactgcttgc cgtcgcatgg atttttgatt
      121 atagagtatt ttactgttga ctaactaaac tcttgggggt aagctataga gtaaagtaac
      181 cggcaatcag cggtaaaata gctatttttc aagattttaa gattgaatat tggattttac
      241 tctacactcg aaaccataga gttgagtaga atatttgacg aatgggaaac acatcatcga
      301 aatttggtgg ttctacactg tgcgggatcg tcagacaccg actgttacct ttaagttgca
      361 tccgtgaaaa caaggggcca aggcaggaca gagtaatcac tggtcggcgt tggaagcctg
      421 aagaaggccc cggacacgaa cctctcgtgt ccccacaggt tgctacctcg gccttcttcc
      481 gctgtgggct taagcgttac cccacgtagc cgctgcgctg ccaaatgaaa tgtcctcatc
      541 acgatgacag cgatgcaaac tacacagaca caccaagacc gaggtgaccg cgagtagctg
      601 acgctactcc agaccaatga ctcacaggta cagactcaaa cgacaatccg ggtccgatgc
      661 tgccaggctt cgccccggat gtcgttgtga agcatcgtcg cagcggacca gcagagccgg
      721 tcctcaacac ggatggagcg gtattatcag tacgctccta tctacaatgt ggacgcgagg
      781 ggtcaaattg gtttcgggac cctcgcattt gccggcttcc acgcagtccg cacttctcca
      841 cggactgcat gcacacgcct aatcaacaac aaggaatccg gaagatcgtt ccaggaggga
      901 ccctctctac tgctggaatt accattaccg aggtcacccc acgggtaaca gaatggatac
      961 ctgaccttct tgaggaacta ctgccacgga gtattcaatc ggttcgaaaa ttcatccggc
     1021 aagaagaccc agaagtcctc acgcacgcac gatacaacac cgtctatcgc cgtcttcaag
     1081 aggaaaccct gaggttcgac catcaagaat ggtgctccac aacggatatt tggagtgatg
     1141 cagaggctga agcggttgaa tacgtagaat cacttgtcga gttcgcagtc aaatattctg
     1201 acgttgacga agatgatctc gacgaacttt cggagtatca ccagcagcgg tgcaaatcgc
     1261 ttaaacagac tctcactacg atcagtaccg gacgtggccc actcaacgct ggccttgaag
     1321 ccctcgccaa gggacccgta cgactccacg atgagctcga tgacgcaccg caaccgatca
     1381 cgcttgtcct tgatggcgag ttgtggtcaa aactcgacga tagaggaaca ggtatccgag
     1441 cacttgcagc tatcgccgtg ctcggctcca ccttcgacgt ccgcctggtt atttcaccag
     1501 cgttagacgc cgcgattgag cgacggtatc cagactggta tgactcccat ctccgtctta
     1561 ctgaaacccg tgagacctcc tctgtagaat cagcgggtgg cgacggacaa ccatcggcgg
     1621 agcagctcga ggaagcatgg gaagcaatac agaacctccc agaggaatca gggagactcc
     1681 gtcttctccg gaacctccct atagagggct ctcgagacta tcgtgatctc aagcaggatg
     1741 atgagattga cgtgcaggca gggactgtcg gccgttacat cctcgatcta gaagaattag
     1801 ggcttgtgga tatcgaccgt cgtggacaat acaacagcgc atccctcacc ggcttaggac
     1861 aagtagcagt tgagcagtat gtcaccacgg actaccgggt gatccatccg acccaatcga
     1921 cgctggagac gcatcttacg ccgacccctc agccccaagc aagtacagtg tatcccgcgc
     1981 gatcggacac gagggagggg gatcagcctg ggacagcgga ggattggata gctgcgacag
     2041 gcagtcctag tgagggtgct gactacgttc aatggctcga tgggccgtct ggtgttctcg
     2101 acgcttgggg aatgcatcag cggtaccttg ctggccgtcg agatcgtggt gtcaccctag
     2161 tcgatgaccg tatcgagcgt ttcgaggacg gtcgcgtatc gtatctcagt tgcttcgacg
     2221 atgatctctt cgtagccact cagtggggcg gcccccttcc taccctggga cgtattgcgg
     2281 gtgccttact cagcgacaaa gcactgagta agatccttac cccttctcgt ctcggaaacc
     2341 agttcgaaga aatcaacgat gcggtcgttg aacaactcga tagagaagct ggggaaatca
     2401 tccgtagagg acaccaaatc ggctggttca gcgaggacga agaagactac gatggttggc
     2461 gtgagcgtat tggctccgtt cgcagtctgt gtttgcaaca agttggtgaa ctcacaaaca
     2521 gtgacgacgt cgaagcccgg actgagttac tacgtgacct gcacggcttg gttgcctcag
     2581 cgacacagct gtactacgca gccggtgtag acgtcacaat caacgttcga gtccctgaca
     2641 cgggaatgct catcagcgat gaacgccgtc tcgacgattt cctcggtttc gcccggtaca
     2701 ccattccgaa acagtccgtg tacgggattc attcggggta taggatgctc ctcgaggatc
     2761 gaccggagaa actcaaacga cgtcttccct acgaggtgga tgatgctgat tccacaatgc
     2821 atctgaccgc atcgtgggtt ttctccggat caacgatgat cgatcttcac gatgacatcg
     2881 aggacgccat tgaaatggag accaacgaaa ttcgtgaagc aattgctaat ggacaggagt
     2941 cagcaccggt aatggaaatc cccgtccaga ttgggaactc ctactcagca attcgtaatc
     3001 acgtcgaaga ctacgcttca gcgaagaact accaggtagc tcaccaggag gatattcacg
     3061 aaggaaaaca ggatctcgag cgactcgtcc gcctgttcct ccgtgtcctc gggacagagg
     3121 atcgaccaca tcgagcgtgt cctcacgacg ttgccgaggc aatgcttcat gttgctcaat
     3181 cctcccggaa ctacgatttc atcaccgtgc gagacatctc gtacggactg tcgaatctcc
     3241 ccacgaaacg actcttaccc gagctcccac caacagcaac gaagctcctc aaaaccctgc
     3301 ttgatgcgga tgacccgatg ggacggtctg aaatcatcga taccgctgac atttcggaaa
     3361 gtagctatga tcgctacatc aacgaactcg ccgcttggga tatcattgaa ccccgcgaaa
     3421 tcgagggaca ccgtcggtgg gaagctcact tagagccgtg gtggacaccg cagagtgatc
     3481 gagacgaacc ctatgccgac cctgaccccg acacgggaat actgtacgct gaatttcccc
     3541 gtgatgtcgc tagtgcggtg atgtgccacc tcatcaccca ctacgactta cccgaccttg
     3601 agacagcgta tcttgagggt atccaaccgg gggacgacat caaggccctc ttcgatgatc
     3661 acgaccgact cagacgatgg cggccattcc tctggggtgc attcgccgac tcggacaaac
     3721 ttgaaagagg cccatctggt acagcagctt cggactcgac cgtagttcgt ctcggtcaat
     3781 ctccaggacc cgacacagca caatcgagct tccaagacgt ctcagaaaca gcaacccaac
     3841 gagatcgtct cagtcaaccg tcgccagggc tggactaatg attgacggca atcgataact
     3901 acgcaatgaa tcctccacaa ccagcatctg aaccgtactc gaaaggtgat gcagtaaccg
     3961 tatacgtggg cgaagacgac cccgatgtcc ggtaccacgg cgttaaatgc gtcgtcactg
     4021 accgcctgca agatgatttg aacaccgaga caggccgtga ccgtgaccag tacctctacc
     4081 gcataaaaag acggtcaacg ggcaaggttc agtacctcac aaagcattct cggctagctg
     4141 tttctgaagc ctgagttcca cggcggagct gttgtgctgg tggtcttgac gagaagatcc
     4201 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4261 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4321 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4381 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     4441 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     4501 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     4561 cgcgcaatta accctcacta aagggaacaa aagctgggta ccgggccccc cctcgaggtc
     4621 gacggtatcg ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga
     4681 gcggccgcca ccgcggtgga gctccaattc gccctatagt gagtcgtatt acgcgcgctc
     4741 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4801 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4861 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4921 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4981 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5041 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5101 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5161 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5221 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5281 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     5341 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5401 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5461 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5521 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5581 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5641 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5701 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5761 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5821 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5881 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5941 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6001 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6061 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6121 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6181 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6241 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6301 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6661 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7141 ttttgctggc cttttgctca catggatcga tcccccagct tcttctagag gtaccgcgtg
     7201 tgaagagtgg catgggaggg gatggggtgt ggacacgccc gcgggtgctc ccgacggacg
     7261 cattttcatc gtttccgcgg aaacggaata aacgcgaccc acaggtgcgg agtgaaagtg
     7321 aaagtgaaac gtgacgaggt gcgaagtcgg aggaacagac cggaacaacg gcgaagaaaa
     7381 ggcagtccag ccgtccctgc tgtcggtgaa aatgaacccg cgagttaccg accgagttcg
     7441 gcgtgggcgc tggagaggtg ccgcgaggcg agcgcggaga gaagggagag ttcgcccgcg
     7501 agggaaccga cggcgatgca ttcggcgagg gcgtcggcgt tggagccggc ggggtcgccg
     7561 ccgccgctga cgccgaggat atcgaggccc tcggactgcg tcgggagttt cgtgccgccg
     7621 ccgacggtgc cgacttcgag ggaggcgatg gagaccgaga cgtagaggtc gccgtcctgc
     7681 acctcggcgg tcgtgatggc gttcgcgccc tcgacgacct gcgcctcgtc ctgcccggtg
     7741 gcgaggaaca tcgcggcgac gacgttggcg acgtgggcgt tgaacccgag gctcgcggcc
     7801 ttcgcggagc cgaccaggtt cttgcgtgtg ttgagttcgc gaccgcgttc gggcgtggtg
     7861 tgcaggcgtt cttcgacgac ctcgcgcggg attcgaacgt cggcggtgac gctccggccg
     7921 cgcccctcga cggcgttgat ggcggcgggc ttcttgtcgg aacagaggtt gcccgagagg
     7981 gcgacgagcg aggcggccgt ctcggcttcg acgacgccgc agacggcctc ggtggcgatg
     8041 gtggccatgt tcatccccat cgcgtccttg gtgtcgtagc ggaatcgcag gtacaccgag
     8101 ttgccgacga cgtacggcgt cacgtcgagg agttcgccgt ggttcgtcgt ctcctccgcg
     8161 gcctccttca gcgccgcgaa gttgtcgcgg gtccacgaga cgagcgcctc ggcctcggca
     8221 acgtcggcga cgcggaacac cggcgcgcgg gtcatcccgg acttgaggac gcgggcggtc
     8281 gcgccgccgg cgctgttgat gaccgagcaa ccgcggttga ccgacgcgag gagcgcgccc
     8341 tcggtggtcg cgagggggag gtacttctcg ccggcgacgg agccgccgtc gacgctgacg
     8401 gggccggcga cgcccatcgg cacctggatc gagccgacca tgttctcgat ggcggactcg
     8461 gcggcctccg cggggaagcc gtagttcccg accgcgtcga gcgacgcgcc ggactgcgat
     8521 tcgacgagca gtcggcgcgc ctcggcggcg gtgtcggcgt cggcgtgcgc ttcgagttcg
     8581 tggaggcgga ggtcgccctc ccgaacgcgg tctgcgaggg acgcggcgtc tgtcatgctc
     8641 cgacgtttcc cgcgccggcc ctaaagcgtt ccggttcttc caacggccgc caccgacccg
     8701 gccggcgtca cccgcgacga ccgaccgccg ctcggccacc ccgccgcctc ccgaaccgtt
     8761 ttgccctccc gcgtcggggt cgaacgtatg accgcggcag cggacctcgt actgacgaac
     8821 gcggaggtac acaccctcgg gtcgcccgac gagacgcacg aagccgtccg tccgcgacgg
     8881 cgaggtcgtc cgcgtcggcc gcgcgttcga cgtgggactg ctcgccggcg tcgagacgcg
     8941 ggtcgtcgac tgcggcggcc
//
