LOCUS       40924_33142        4172 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNG10A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, 
            Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
            Munoz-Dorado J.
  TITLE     Comprehensive Set of Integrative Plasmid Vectors for 
            Copper-Inducible Gene Expression in Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
  PUBMED    22287008
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Gomez-Santos N.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
            Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE   3  (bases 1 to 4172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "8"; pages:
            "2515-2521"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-OCT-2011) Microbiology, University of Granada (Spain), Avda. 
            Fuentenueva s/n, Granada, Granada 18071, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..973
                     /label=cuoA promoter
                     /note="cuoA promoter"
                     /regulatory_class="promoter"
     misc_feature    974..1008
                     /label=multicloning site
                     /note="multicloning site"
     primer_bind     complement(1021..1037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1045..1061)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1069..1099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1114..1135)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1423..2011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2139..2167
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2215..3402
                     /label=TcR
                     /note="tetracycline efflux protein"
     primer_bind     4144..4160
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtaccgctg cttcaacggc attcatgggg cttcctcctg cccgcgagct agcgggtcac
       61 cccactgacg cgcctccccc gaaaggctta cacctgcgaa gagggccagc cgcggtggcc
      121 agcgcctcgt gcgccacccg cagcgtgtcg gccatgaacg gcacttcacg gctccgtgga
      181 acgacgctgc tccacgttcc acctgtgacc gtgacggtta cagcgaaacc catccgggta
      241 ctccggacag gtgccgcgcc acggcaaccc cgcatgaaca ggctcgctca gccctcggca
      301 cgcgttgtgc tccagcggac cgacttcacg ggatgccgga cgtcctcgag agcatcctca
      361 ctctgggagc aaccaagcga tgaagaagag cctgatggga ctccttgctg cactccccct
      421 gattgccgcg gcccccgcgg tcgcgtgtga cgagcacgcc aagcacggcg agcacgccag
      481 ccacggcgag cacgccaagc acggcgatca ttccgcgctc ggaggcacgg ccaagaaggc
      541 cgctgcgccc gcacagaagc ccgcctcggc cccggccacc aggaatggcg tccagaccgt
      601 ggacctcacg gtgaccccca agggcttcga gccctcggac gtgaaggtga aggcggggca
      661 gcccgtccgc ctcgtggtca cgcgcaagac ggccaagacg tgcgccacgg aaatcgtcat
      721 ggccgacctg ggcatcaaca agccgctccc catggacacg ccggtgaccg tggagttcac
      781 ccccagcaag tcgggcacgc tgcgctacgc ctgcgcgatg gaccacatca gcgggctcgt
      841 caccatccag tagaccgccg ttccgggcag gctgcgcacc gaggtcacgc gcctcggggc
      901 cgggccacgc caccacggcg gcctggtccc ctcctcgccc gcagcttcca tccattcgtg
      961 aagaggcttc atggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg
     1021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2101 gatcttcacc tagatccttt ttcgaccaat tctcatgttt gacagcttat catcgataag
     2161 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
     2221 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
     2281 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
     2341 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
     2401 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
     2461 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct ctacgccgga
     2521 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     2581 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     2641 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt acatgcacca
     2701 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     2761 gagtcgcata agggagagcg ccgaccgatg cccttgagag ccttcaaccc agtcagctcc
     2821 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     2881 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     2941 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     3001 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     3061 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     3121 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     3181 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     3241 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
     3301 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     3361 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     3421 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     3481 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     3541 gcacgcggcg catctcgggc agcgttgggt ccctggggtc gaattattga agcatttatc
     3601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3721 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     3781 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3841 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3901 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     3961 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     4021 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     4081 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     4141 gttgtaaaac gacggccagt gaattcgagc tc
//
