LOCUS       V004336                 8190 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004336
VERSION     V004336
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Pisciotta
            A, Alduina R, Manteca A.
  TITLE     New PhiBT1 site-specific integrative vectors with neutral phenotype
            in Streptomyces
  JOURNAL   Appl. Microbiol. Biotechnol. 100 (6), 2797-2808 (2016)
   PUBMED   26758297
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Gonzalez-Quinonez N, Lopez-Garcia MT, Yague P, Rioseras B, Manteca
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2015) Biologia Funcional, Universidad de Oviedo,
            c/Julian Claveria s/n Facultad de Medicina, Oviedo 33006, Spain
REFERENCE   3  (bases 1 to 8190)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8190)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2016"; volume: "100"; issue: "6";
            pages: "2797-2808"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-APR-2015) Biologia Funcional, Universidad de Oviedo, c/Julian
            Claveria s/n Facultad de Medicina, Oviedo 33006, Spain"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      275..863
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1151..1172
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1187..1217
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1225..1241
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1249..1265
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     oriT            3490..3599
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             3632..4000
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(4716..5711)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     CDS             complement(6396..7706)
                     /codon_start=1
                     /gene="SCO4849"
                     /product="SCO4849 protein"
                     /label="SCO4849"
                     /protein_id="AML61761.1"
                     /translation="MVVVFVLVALLMVGVLVTANWYVWRRLFRDTTRAPGPVRRIGAAV
                     IAGGWLLAVGALVAERAGAPFWLQRVLAWPGFLWLALSIYLLLAVLAGEVVRPLLRRFL
                     ERRAAARRTAGAEPSVTPAADTSRIPAGTAPPKTPEAPETPEAPGTEALAAQGSPLAVP
                     SRRLFVSRVVAGAAAAAAVGTVGYGTYGVLSGPKVKRVTVPLAKLPRAAHGFRIAVVSD
                     IHLGPVLGRGFAQQVVDTINSTQPDLIAVVGDLVDGSVKDLGPAAAPLARLTARHGAYF
                     VTGNHEYFSGAEQWVAEVRRLGLLPLENARTELPHFDLAGVNDVAGEDEGQGPDYDRAL
                     GDRDRSRACVLLAHQPVQIHDAVDHGVDLQLSGHTHGGQLWPGNLIAGAANPTLAGLER
                     YGDTQLYVSRGAGAWGPPTRVGAPSDITVIELASRQA"
     gene            complement(6396..7706)
                     /gene="SCO4849"
                     /label="SCO4849"
     regulatory      complement(7767..8189)
                     /regulatory_class="promoter"
ORIGIN
        1 agcttggatc ctaggttctc ggcttttcgc cattcgtatt gcacgacatt gcactccacc
       61 gctgatgaca tcagtcgatc atagcacgat caacggcact gttgcaaata gtcggtggtg
      121 ataaacttat catccccttt tgctgatgga gctgcacatg aaccaaaagg atctaggtga
      181 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
      241 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      301 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      361 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      421 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      481 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      541 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      601 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      661 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      721 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      781 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      841 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      901 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      961 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1021 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     1081 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     1141 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     1201 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     1261 atgacatgat tacgaattcg atatcgcgcg cggccgcgga tccgctccct gcccgctatg
     1321 gtgacgaagg aactactagt tagcctaact aacgatttca aggccaaaaa aggggccgaa
     1381 ccgtccgccg gctcgacccc tcccgctgtg cgctacagcg ccgcaagctc ccgctcagtg
     1441 gcttcgctcg cttcgtcttc ctccttcagc agctccgccc acttgagcgt cacacggtcc
     1501 ttcaggggga tgtatttctt cgtctcaggg tggcgccccc ggtcgaccat gacggagtca
     1561 aggaagaagc tcaggaactc ccgacgctcg tacacgtccc agcttgccca gatgccccct
     1621 tcggccgtcg ggtcttcgcc gctgaaccac tcagacggaa cgcgggtgct gccgttcatc
     1681 ttctcgtcaa gctcagcgag tcgggtcgtg cactccgctt cgtaggaccg gtattgcagc
     1741 accgttgagc gccacgtttc cagctcttca cgcccgacgt acagaccggc cttacggtcc
     1801 gcctgaaggt ccttgatgga gcgccgcacg ttgtctaggt gcgcctgttg ttcgcgccgc
     1861 tcatcggcca cccccgctag gtcgtgctga agggcgaagc gctccgcagc ggcggcaatc
     1921 catgcctgat cgtgttcatc ttccatgtcg gctgtgcgga gccgtgccca caccttcgaa
     1981 gcaacgaatt cgtccagttc gctgcgcttg accgacaagc cgccgtgccc cttcgggttg
     2041 gcgcacatgt aattgccttc ggcaaggtcg ccgttgcgct tacggccacc ctgggactga
     2101 cccattgagc cgccgcagat tcggcacccc aggaaacgcc acccgctcag aagcgtcggt
     2161 tcgacttcgg ccccaggctt ccggtcgctg agattcttcc cggaacgctt ctcttgaagc
     2221 tcaagccact tcgagccgct gagaatgccc gtgtggggcg ttagcggctt gccgccgggg
     2281 tcgcgccgta tgacgttgat gtgcgcctta ccgtgcttca cacgctcgaa tgcgaaaccg
     2341 ccgattgcgg gatggttgag aatccatcgg accgtttgag cgcgccacat gatcggcttt
     2401 tcagcgccgt tcaggcgacg tgccttgatg gacgcaagac gcttttccgt ggcgcgtctc
     2461 tcagccattc cgggcgacgg gatcttttcc ttctcgaagg tcgttgcaat ggcgttgtcg
     2521 gacacgcctt cgaacgacat tttcgccatg cgctcaacta gctcgacgtg atccgggttg
     2581 tcttcgtccg gctcaagaac ggagatcacg agattatcga ccttcttgcg cacggcgcgc
     2641 attccgaacg gggcggaaga cgagtgaacg ccacccagcg cggcaatctc gtctttcgca
     2701 cccttcaggc gctccgcctt caggtcactg tcctgtttcg caagggcagc gatcagcgcg
     2761 aaaatggcga cgccgatagg ggtagacgtg tcaaggaacg gctcaagaac cgacatgaag
     2821 cgcacgccgt gcttcttcaa ttcgttgtcg atttcgagcg cgtcatgggc gcccttgcga
     2881 gtgagccggg aaagctcatt cacaacgaca acgtcacctt cgccggcgcg gacttcgccc
     2941 atcatgcgct cgaagtcggc acgggtcacg ttcgggtccc agccggagcg cccgacgtcc
     3001 acgaactcac ctgccacgac ccagcgcgca cccccctggg cgttgcgaga cgcgaccaac
     3061 gcacgaccgg ccgctagctg tgcttcggtc gacacgtctg agccgtcgga ccggccctta
     3121 gactgacgcg cgtacaggaa gacgcgaaca gtgtctagaa ggtgctcagg gacgtcggga
     3181 gcgatgaagg gcgacatgcc tagatcttgg ctcatgggcg ctgacctgcg ctttcttttg
     3241 ggaacattgg tggtgctgag tagtttccca tggatcactg tccagagaca acaacccagc
     3301 acccgaaacg tctgaagccc cgtccggcgc cagcatgcct gcaggtcgac ggatcttttc
     3361 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
     3421 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
     3481 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
     3541 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc
     3601 taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa
     3661 gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa
     3721 ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa
     3781 aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct
     3841 gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt
     3901 cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg
     3961 caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa
     4021 acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg
     4081 acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgat ccccggggac
     4141 ctgcaggtcg actctagcta gctggccagg gcccgatacg tcgcggtgag ttcaggcttt
     4201 ttcatatctc attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa
     4261 tcggatcagc cgtccaaatg cgggattttc gttagtcgga ggccaaacgg cattgagcgt
     4321 cagcatatca tcagcgagct gaagaaagac aatccccgat ccgctccacg tgttgcccca
     4381 gcaatcagcg cgaccttgcc cctccaacgt catctcgttc tccgctcatg agaactgaga
     4441 agccccgtca ggcaccgccc gacggggctc ttcacccggg ttgccgagct gggatggaag
     4501 ctcggccgac caccctggag gagatgatcg aggatgccag ggcctttcac gcccgccgct
     4561 gctgagcgtc cgccgccggg cccgcaccgc cgtcggccgg cccgctccgg gctcgcagca
     4621 gcgggcttcg gcgcgggccc ggggctcccg agcgcgggcg gggctccggg cggccgccgg
     4681 gggccggggg cggcgccggg cggcccgggg cgtcaggcgc cgggggcggt gtccggcggc
     4741 ccccagagga actgcgccag ttcctccgga tcggtgaagc cggagagatc cagcggggtc
     4801 tcctcgaaca cctcgaagtc gtgcaggaag gtgaaggcga gcagttcgcg ggcgaagtcc
     4861 tcggtccgct tccactgcgc cccgtcgagc agcgcggcca ggatctcgcg gtcgccccgg
     4921 aaggcgttga gatgcagttg caccaggctg tagcgggagt ctcccgcata gacgtcggtg
     4981 aagtcgacga tcccggtgac ctcggtcgcg gccaggtcca cgaagatgtt ggtcccgtgc
     5041 aggtcgccgt ggacgaaccg gggttcgcgg ccggccagca gcgtgtccac gtccggcagc
     5101 cagtcctcca ggcggtccag cagccggggc gagaggtagc cccacccgcg gtggtcctcg
     5161 acggtcgccg cgcggcgttc ccgcagcagt tccgggaaga cctcggaatg gggggtgagc
     5221 acggtgttcc cggtcagcgg caccctgtgc agccggccga gcacccggcc gagttcgcgg
     5281 gccagggcga gcagcgcgtt ccggtcggtc gtgccgtcca tcgcggaccg ccaggtggtg
     5341 ccggtcatcc ggctcatcac caggtagggc cacggccagg ctccggtgcc gggccgcagc
     5401 tcgccgcggc cgaggaggcg gggcaccggc accggggcgt ccgccaggac cgcgtacgcc
     5461 tccgactccg acgcgaggct ctccggaccg caccagtgct cgccgaacag cttgatcacc
     5521 gggtcgggct cgccgaccag tacggggttg gtgctctcgc cgggcacccg cagcaccggc
     5581 ggcaccggca gcccgagctc ctccagggct cggcgggcca gcggctccca gaattcctgg
     5641 tcgttccgca ggctcgcgta ggaatcatcc gaatcaatac ggtcgagaag taacagggat
     5701 tcttgtgtca cagcggacct ctattcacag ggtacgggcc ggcttaattc cgcacggccg
     5761 gtcgcgacac ggcctgtccg caccgcggat caggcgttga cgatgacggg ctggtcggcc
     5821 acgtcgggga cgttctcggt ggtgctgcgg tcgggatcgc caatctctac gggccgaccg
     5881 aggcgacggt gtacgccacc gcctggttct gcgacggcga ggcgccgtcc aggccccgcc
     5941 cgatccccgt cccccgcgtc gtcgagcgcg gtgccgacga caccgccgcg tggctcgtca
     6001 cggaggccgt ccccggcgtc gcggcggccg aggagtggcc cgagcaccag cggttcgccg
     6061 tggtcgaggc gatggcggag ctggcccgcg ccctccacga gctgcccgtg gaggactgcc
     6121 ccttcgaccg gcgcctcgac gcggcggtcg ccgaggcccg gcggaacgtc gccgagggcc
     6181 tgtggacctc gacgaccgat ctgatgtgct cagtatcacc gccagtggta tttatgtcaa
     6241 caccgccaga gataatttat caccgcagat ggttacctcg cctctgaccc ctgaccccgt
     6301 cagcctgagg ttgaaaaacg ctcactggta ccatgcatgg ggatgacgaa ggtgatgccg
     6361 gacccggcga agaaaccgac gcgggatgcc ggtcctcagg cctgacggga cgccagctcg
     6421 atcaccgtga tgtccgaggg cgcccccacg cgcgtgggcg ggccccaggc gcccgcgccg
     6481 cggctgacgt agagctgggt gtcgccgtag cgctccaggc ccgccagcgt cggattggcg
     6541 gcgcccgcga tgaggttgcc gggccagagc tggccgccgt gggtgtggcc ggagagctgg
     6601 aggtcgactc cgtggtccac ggcgtcgtgg atctgcaccg gctggtgggc gaggagcacg
     6661 caggcgcggg atcggtcccg gtcgccgagg gccctgtcgt agtcggggcc ctggccctcg
     6721 tcctcgccgg cgacgtcgtt gacgccggcc aggtcgaagt ggggcagctc ggtgcgggcg
     6781 ttctccagcg ggagcagccc gagccgccgc acctcggcga cccactgctc ggcgccggag
     6841 aagtactcgt ggttgccggt gacgaagtac gcgccgtgcc gtgccgtcag ccgggccagc
     6901 ggggcggcgg ccgggccgag gtccttcacg ctgccgtcca ccaggtcgcc gacgacggcg
     6961 atcaggtcgg gctgcgtgga gttgatcgtg tcgacgacct gctgcgcgaa gccccggccg
     7021 agcaccgggc ccaggtggat gtcgctgacg acggcgatgc ggaagccgtg cgccgcgcgc
     7081 gggagcttgg ccagcggcac cgtgacccgc ttcaccttcg ggccgctcag cacgccgtac
     7141 gtgccgtagc cgaccgtgcc gacggccgcc gccgcggcgg caccggcgac gacgcgggag
     7201 acgaagagac ggcgggaggg tacggcgagg gggctgccct gggctgccag ggcttcggtc
     7261 cccggtgcct cgggcgtctc aggtgcctcg ggcgtcttcg gcggagccgt gccggccggt
     7321 atgcgcgagg tgtccgcggc cggggtgacc gagggctccg cccccgccgt ccgccgcgcg
     7381 gccgccctgc gctccaggaa ccgccgcagc agcggccgga cgacctcgcc cgcgagcacc
     7441 gcgagcagca ggtagatcga cagggcgagc cacaggaagc ccggccaggc gagcacccgc
     7501 tggagccaga acggggcgcc cgcgcgctcg gcgaccagcg ccccgaccgc cagcagccag
     7561 cccccggcga tcacggccgc gccgattcgg cgcacgggac ccggggcgcg ggtggtgtcg
     7621 cggaacagac gccgccacac gtaccagttg gccgtcacca ggacgcccac catcagcaac
     7681 gcgaccagca cgaagacgac caccatgccg tcgtatcccc tcggttggga cgctcagggc
     7741 gtccgtgacg tcctcgtcgc catatgggtt tcagtatcgc cgcacgctgt ccggatccgt
     7801 gcgcggggtg gggagggggg tgggaccgac ccgatcgcgg ggggtacgtt tccgctcgtg
     7861 cccgctccca agaaggccct caggcgatcc ctgacggtca cctccgctgc cctgaccgct
     7921 ctcgccctcc tctcccccgc cgcgctcgcc gccccgagcc cgtcgacgag tccgtcggcc
     7981 actccgccgg cgtcgatgtc gggcgtgggc ggtgcgcggc tcgggaagcc cggaacgcag
     8041 gtggacctgg cgggcggggc gcccgtgctg ccgaaggacc tcacggcccg gtcgtggatc
     8101 gtcgcggacg ccgagtcggg cgacgtgctg gccgcgcaca acgcgcactg ggagctggcc
     8161 ccggcgagca ccctgaagat gctgttcgca
//
